MCQOPTIONS
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MCQOPTIONS
This section includes 10 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Gene V blocks the production of which gene? |
| A. | Gene III |
| B. | Gene II |
| C. | Gene VIII |
| D. | Gene IV |
| Answer» C. Gene VIII | |
| 2. |
Replication can also be carried out by rolling circle replication. Choose the correct statement for this type of replication? |
| A. | It can be used for the formation of RF |
| B. | The product of gene III binds on a specific site of double stranded genome |
| C. | It creates a nick in the – strand generating a free 3’ hydroxyl |
| D. | This strand is extended by the polymerase and displaces the original – strand |
| Answer» B. The product of gene III binds on a specific site of double stranded genome | |
| 3. |
Once the bacteriophage enters the bacterial cells, some changes are carried out in it. Which of the following is not included? |
| A. | The single stranded molecule entering the cell is single stranded and is called as a + strand |
| B. | The single stranded molecule is converted into double stranded molecule which is called as replicative form (RF) |
| C. | It is carried out in a process similar to normal replication process of E. coli |
| D. | The positive strand is used for the transcription of viral proteins |
| Answer» E. | |
| 4. |
Choose the correct statement for the infection process of M13. |
| A. | The infectious particle is double stranded |
| B. | It is contained in a protein coat which is made up of products of gene III or gene VIII |
| C. | The phage infects only female cells |
| D. | The phage enters the bacterial cells by the filament which is meant for movement |
| Answer» C. The phage infects only female cells | |
| 5. |
The bacteriophage M13 vectors belong to a group of phages called as __________ |
| A. | skinny or filamentous phage |
| B. | M phage group |
| C. | single stranded phage |
| D. | double stranded phage |
| Answer» B. M phage group | |
| 6. |
Choose the correct statement for BAC vector system. |
| A. | BAC vector system stands for bacteria and chromosome |
| B. | It usually accepts insert of size approximately 1000kbp |
| C. | The repE and oriS sequences are required for controlling the copy number and par A-C sequences are required for replication |
| D. | A selectable marker is there for chloramphenicol resistance |
| Answer» E. | |
| 7. |
The low copy number of F plasmid is essential for the stability of these plasmids. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 8. |
Excision of the F factor can also be done from the host chromosome. Choose the incorrect statement in respect to it. |
| A. | This excision is possible in vivo |
| B. | Along with the F factor, host chromosome’s DNA sequences are also excited |
| C. | The sequences of host chromosome which can be accommodated in the F plasmid are small in size |
| D. | These plasmids are termed as F’ plasmids |
| Answer» D. These plasmids are termed as F’ plasmids | |
| 9. |
F factor integration into the host chromosome is called as __________ |
| A. | F’ plasmid |
| B. | F factor and host chromosome recombination |
| C. | Hfr (High frequency of recombination) |
| D. | Recombinant host chromosome |
| Answer» D. Recombinant host chromosome | |
| 10. |
F (fertility) factor is used in many bacterial systems for different purposes. Choose the incorrect statement with respect to the F factor. |
| A. | It was identified in E. coli for catalysing genetic exchange between cells |
| B. | Cells having F factor are called as female |
| C. | The F factor is transferred from one cell to another via factors present outside |
| D. | They are not related to BAC systems |
| Answer» B. Cells having F factor are called as female | |