MCQOPTIONS
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MCQOPTIONS
This section includes 11 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The main advantage of phagemid is that it can be used for the generation of single or double stranded products without recloning. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 2. |
M13-plasmid vectors are often used. Which of the following is not a feature for this? |
| A. | They are known as phagemid, plage or phasmid |
| B. | Replication takes place through the origin of replication of M13 derivatives only |
| C. | Replication from M13 requires proteins to be provided by helper phage |
| D. | Some of the examples of these types of the combination are pUC 118, 119 and 120 |
| Answer» C. Replication from M13 requires proteins to be provided by helper phage | |
| 3. |
Choose the correct statement for the synthesis of RNA probe? |
| A. | Double stranded DNA is only used for probe synthesis |
| B. | Single stranded DNA can be used for probe synthesis |
| C. | Probes are prepared for RNA transcripts which are specific for only particular strand |
| D. | Probes are prepared for RNA transcripts which are specific for both the strands |
| Answer» C. Probes are prepared for RNA transcripts which are specific for only particular strand | |
| 4. |
Which of the statement doesn t holds for phage display systems? |
| A. | The filamentous phage is often used as phage display system |
| B. | Coding sequences are inserted in one of the protein coating genes |
| C. | The most common protein coding genes are gene II or gene VIII |
| D. | The inserted is expressed along with other protein coat genes |
| Answer» D. The inserted is expressed along with other protein coat genes | |
| 5. |
Choose the correct statement for Sanger s method of sequencing. |
| A. | They are also called as chain termination methods |
| B. | Use of M13 vector is made for getting double stranded DNA |
| C. | Use of dideoxynucleotide phosphate (ddNTP) is made |
| D. | The use of M13 vectors is more preferred these days |
| Answer» B. Use of M13 vector is made for getting double stranded DNA | |
| 6. |
Choose the incorrect statement with respect to the collection of single stranded molecules. |
| A. | An infected culture is set up and after a while the cells are removed by centrifugation |
| B. | The phage is in the supernatant and is precipitated by addition of glycol and sodium chloride |
| C. | The phage pellet is resuspended and phenol is used for removal of protein |
| D. | The single stranded molecules sediment at the bottom |
| Answer» E. | |
| 7. |
M13 vector is tightly packed. Where is the available site to insert DNA? |
| A. | In the gene II |
| B. | Between gene II and IV |
| C. | Between gene III and V |
| D. | Between gene V and VIII |
| Answer» C. Between gene III and V | |
| 8. |
The plaques formed by M13 infection are called as __________ |
| A. | retarded plaques |
| B. | true plaques |
| C. | pseudoplaques |
| D. | lysogenized plaques |
| Answer» D. lysogenized plaques | |
| 9. |
M13 doesn t actually lyse the cells. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 10. |
The uniform layer of cells is known as lawn and the holes peppered from infected cells are termed as __________ |
| A. | plaques |
| B. | holes |
| C. | colony |
| D. | pothole |
| Answer» B. holes | |
| 11. |
What is the size of the DNA molecule which can be packed by the viral protein coat? |
| A. | 100kbp |
| B. | 200kbp |
| C. | 500kbp |
| D. | No size restriction |
| Answer» E. | |