 
			 
			MCQOPTIONS
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				This section includes 38 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.
| 1. | The nucleous of mature unfertilized ovum may be removed by | 
| A. | irradiation | 
| B. | surgery | 
| C. | both (a) and (b) | 
| D. | neutralization and homogenization | 
| Answer» D. neutralization and homogenization | |
| 2. | Karyoplast is | 
| A. | cells devoid of cell wall | 
| B. | nuclei | 
| C. | nuclei with only some residual plasma membrane | 
| D. | cell with nucleus | 
| Answer» D. cell with nucleus | |
| 3. | The success of embryo transplantation depends upon the ability to obtain an increased number of embryos from animals of superior genetic merit, which can be achieved by the use of | 
| A. | exogenous gonadatrophic hormones | 
| B. | endogenous gonadatrophic hormones | 
| C. | both (a) and (b) | 
| D. | FSH (Follicle Stimulating Hormone) | 
| Answer» B. endogenous gonadatrophic hormones | |
| 4. | Multiple ovulation and embryo transfer (MOET) | 
| A. | can increase the rate of progress in dairy cattle | 
| B. | allows progeny testing of males | 
| C. | allows progeny testing of females | 
| D. | all of the above | 
| Answer» E. | |
| 5. | The technique, mainly used for the diagnosing birth defects in the fetus by means of needle, is called | 
| A. | amniocentesis | 
| B. | ectogensis | 
| C. | transplantation | 
| D. | all of the above | 
| Answer» B. ectogensis | |
| 6. | DNA is microinfected into the fertilized egg | 
| A. | after the fusion of male and female nuclei | 
| B. | before the fusion of male and female nuclei | 
| C. | at the time of fusion of male and female nuclei | 
| D. | any time, it can be infected | 
| Answer» C. at the time of fusion of male and female nuclei | |
| 7. | In which year, Dolly the first mammalian clone was born? | 
| A. | 1995 | 
| B. | 1997 | 
| C. | 1996 | 
| D. | 1998 | 
| Answer» D. 1998 | |
| 8. | Embryo transfer (ET) refers to the technique by which fertilized ova are collected from the reproductive tract of a genetically | 
| A. | superior female and transferred to the inferior | 
| B. | inferior female and transferred to the superior | 
| C. | balanced female | 
| D. | none of the above | 
| Answer» B. inferior female and transferred to the superior | |
| 9. | The number of follicles at any particular stage is governed by | 
| A. | the rate of entry of dormant follicles | 
| B. | the rate of growth of follicles | 
| C. | the rate of loss of follicles | 
| D. | all of the above | 
| Answer» E. | |
| 10. | Production of transgenic animals require transfections of | 
| A. | eggs or embryos | 
| B. | stem cells | 
| C. | red blood cells | 
| D. | all of these | 
| Answer» B. stem cells | |
| 11. | Which of the following gene have been introduced into the transgenic fish? | 
| A. | Human or rat gene for growth hormone | 
| B. | Chicken gene for delta crystalline protein | 
| C. | <i>E. coli</i> | 
| D. | gene for -galactosidase | 
| E. | All of the above | 
| Answer» E. All of the above | |
| 12. | Enucleation of the cells can be achieved by treating the cells with | 
| A. | polyethylene glycol | 
| B. | cytochalasin B | 
| C. | both (a) and (b) | 
| D. | alcohol | 
| Answer» C. both (a) and (b) | |
| 13. | When the cells are enucleated and centrifuged, nuclei | 
| A. | detach from cell and settle at the bottom | 
| B. | detach from cell and remain in the supernatant | 
| C. | nuclei remain attached to the cell | 
| D. | none of the above | 
| Answer» B. detach from cell and remain in the supernatant | |
| 14. | Which of the following statements best describes a clone? | 
| A. | An artificial life form | 
| B. | An offspring where all of the genetic material in every cell is identical to that of both parents | 
| C. | An offspring where all of the genetic material in every cell is identical to that of one of its parents | 
| D. | A type of sheep | 
| Answer» D. A type of sheep | |
| 15. | Which of these carries the thousands of genes needed to allow animal and plant cells to pass on inherited characteristics? | 
| A. | Chromosomes | 
| B. | Chromatogram | 
| C. | Chromatography | 
| D. | Chromium | 
| Answer» B. Chromatogram | |
| 16. | In transgenic fish, the genes are introduced by | 
| A. | microinjection in fish | 
| B. | viruses | 
| C. | transfer of whole nuclei | 
| D. | all of these | 
| Answer» B. viruses | |
| 17. | Animal pharming can be defined as | 
| A. | growing animals for farming | 
| B. | programming animals to produce novel products | 
| C. | generating transgenic animals for farming | 
| D. | none of the above | 
| Answer» C. generating transgenic animals for farming | |
| 18. | When a fertilized egg cell develops into an embryo, the entropy of the living system | 
| A. | decreases | 
| B. | increases | 
| C. | remains constant | 
| D. | none of these | 
| Answer» B. increases | |
| 19. | For the transfer of whole individual chromosomes, they are isolated from the cells at | 
| A. | prophase | 
| B. | metaphase | 
| C. | telophase | 
| D. | anaphase | 
| Answer» C. telophase | |
| 20. | Fusion of karyoplast with the enucleated cell is achieved in presence of | 
| A. | cytochalasin B | 
| B. | polyethylene glycol | 
| C. | both (a) and (b) | 
| D. | alcohol | 
| Answer» C. both (a) and (b) | |
| 21. | Transfection refers to which of the following? | 
| A. | Synthesis of mRNA from DNA template | 
| B. | Synthesis of protein based on mRNA sequence | 
| C. | Introduction of foreign gene in to a cell | 
| D. | The process by which a cell become malignant | 
| Answer» D. The process by which a cell become malignant | |
| 22. | Chromosomes may be isolated from metaphase cells by | 
| A. | hypertonic lysis | 
| B. | hypotonic lysis | 
| C. | either (a) or (b) | 
| D. | isotonic lysis | 
| Answer» C. either (a) or (b) | |
| 23. | Superovulation is primarily the result of | 
| A. | decreased atresia in medium and large follicles (>1.7 mm) | 
| B. | increased atresia in medium and large follicles (>1.7 mm) | 
| C. | increased atresia in the large follicles (<1.7 mm) | 
| D. | decreased atresia in small follicles (<1.7 mm) | 
| Answer» B. increased atresia in medium and large follicles (>1.7 mm) | |
| 24. | Genetic change can occur only in | 
| A. | one way | 
| B. | two ways | 
| C. | three ways | 
| D. | four ways | 
| Answer» E. | |
| 25. | Superovulation is an | 
| A. | increased ovulatory response by external hormonal therapy | 
| B. | decreased ovulatory response by external hormonal therapy | 
| C. | decreased ovulatory response by internal hormonal therapy | 
| D. | increased ovulatory response by internal hormonal therapy | 
| Answer» B. decreased ovulatory response by external hormonal therapy | |
| 26. | Which of the following best describes artificial insemination? | 
| A. | Transplanting an embryo into the uterus | 
| B. | Fertilization of an egg in a test tube | 
| C. | Selectively breeding healthy animals | 
| D. | Taking the sperm and placing it directly | 
| Answer» E. | |
| 27. | Which part of a cell carries the information that is passed from one generation to the next? | 
| A. | Cell | 
| B. | Nucleus | 
| C. | Cytoplasm | 
| D. | Chloroplast | 
| Answer» C. Cytoplasm | |
| 28. | Transgenic goats produce a variant of human tissue type plasminogen activator protein in | 
| A. | blood | 
| B. | urine | 
| C. | milk | 
| D. | muscles | 
| Answer» D. muscles | |
| 29. | For the rapid genetic progress, embryo transfer (ET) | 
| A. | will be as potent a tool as AI (artificial insemination) | 
| B. | will not be as potent a tool as AI (artificial insemination) | 
| C. | ET can't be used at all | 
| D. | will be a superior tool than AI | 
| Answer» C. ET can't be used at all | |
| 30. | Cloning is a method by which numbers of genetically identical organisms are derived from a single organism by | 
| A. | vegetative propagation | 
| B. | vegetative initiation | 
| C. | vegetative termination | 
| D. | none of these | 
| Answer» B. vegetative initiation | |
| 31. | Which of the following is/are the method of transfection for making transgenic animals? | 
| A. | Transfer of whole nuclei | 
| B. | Transfer of whole individual chromosomes or fragment | 
| C. | Transfer of DNA | 
| D. | All of the above | 
| Answer» E. | |
| 32. | DNA microinjection into the egg has been used to produce which of the following transgenic animals? | 
| A. | Mice | 
| B. | Chicken | 
| C. | Pigs | 
| D. | All of these | 
| Answer» E. | |
| 33. | Which of these established cell lines originate from a mouse embryo? | 
| A. | 3T3 | 
| B. | BHK | 
| C. | HeLa | 
| D. | BTK | 
| Answer» B. BHK | |
| 34. | Some types of variation are due to changes in the genetic material. What is this type of change called? | 
| A. | Fertilisation | 
| B. | Mutation | 
| C. | Radiation | 
| D. | Sterilisation | 
| Answer» C. Radiation | |
| 35. | Which of the following is the process of choosing parent organisms for the characteristics that is wanted in their offspring? | 
| A. | Active selection | 
| B. | Reproductive selection | 
| C. | Selective breeding | 
| D. | Breeding selection | 
| Answer» D. Breeding selection | |
| 36. | Which protein has been produced generating a transgenic sheep that is used for replacement therapy for individuals at risk from emphysema? | 
| A. | Plasminogen activator (tPA) | 
| B. | -anti trypsin (AAT) | 
| C. | Casein | 
| D. | Amyloid precursor proteins | 
| Answer» C. Casein | |
| 37. | Which of the following is the word used to describe what happens when the nucleus of a sperm joins with the nucleus of an egg cell? | 
| A. | Sex | 
| B. | Fertilisation | 
| C. | Intercourse | 
| D. | Reproduction | 
| Answer» C. Intercourse | |
| 38. | DNA into fish is injected into | 
| A. | pronuclei | 
| B. | cytoplasm | 
| C. | both (a) and (b) | 
| D. | none of these | 
| Answer» C. both (a) and (b) | |