Which one of the following is not done if amplification of the non flanking region is carried out?

Question

TuteeHUB · Accepted Answer

NA

TuteeHUB · Answer

Firstly, restriction enzyme digestion is done of those sites whose sequence is not known

TuteeHUB · Answer

Then the self-ligation of molecules is allowed

TuteeHUB · Answer

Now the molecules are cleaved where the known sequence is

TuteeHUB · Answer

Again, the molecules are linearized and the known sequence is in the middle

1.	Which one of the following is not done if amplification of the non flanking region is carried out?
A.	Firstly, restriction enzyme digestion is done of those sites whose sequence is not known
B.	Then the self-ligation of molecules is allowed
C.	Now the molecules are cleaved where the known sequence is
D.	Again, the molecules are linearized and the known sequence is in the middle
Answer» E.

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