Which of the following is incorrect about Yeast-two-hybrid screens? – McqOptions

MCQOPTIONS

1.	Which of the following is incorrect about Yeast-two-hybrid screens?
A.	The yeast-two-hybrid system uses the transcription of a reporter gene driven by the Gal4 transcription factor to monitor whether or not two proteins are interacting
B.	The DNA-binding domain chimeric protein will not bind upstream of the reporter gene
C.	If the activation domain chimeric protein interacts with the DNA-binding domain chimeric protein, the reporter gene will be transcribed
D.	Disadvantages of the method are that only pairwise interactions are tested, and not interactions that can only take place when multiple proteins come together, as well as a high false positive rate
Answer» C. If the activation domain chimeric protein interacts with the DNA-binding domain chimeric protein, the reporter gene will be transcribed

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