Which of the following does not describe local alignment?

• A. A local alignment aligns a substring of the query sequence to a substring of the target sequence
• B. A local alignment is defined by maximizing the alignment score, so that deleting a column from either end would reduce the score, and adding further columns at either end would also reduce the score
• C. Local alignments have terminal gaps
• D. The substrings to be examined may be all of one or both sequences; if all of both are included then the local alignment is also global