Two considerations arise in trying to tune the PSSM so that it adequately represents the training sequences. Which of the following is not their description?

• A. If a given column in 20 sequences has only isoleucine, it is not very likely that a different amino acid will be found in other sequences with that motif because the residue is probably important for function
• B. If a given column in 20 sequences has only isoleucine, it is very likely that a different amino acid will be found in other sequences with that motif because the residue is probably important for function
• C. If the number of sequences with the found motif is large and reasonably diverse, the sequences represent a good statistical sampling of all sequences that are ever likely to be found with that same motif
• D. Another column in the motif from the 20 sequences may have several amino acids, and some amino acids may not be represented at all