Topoisomerse I is also used for cloning of PCR product at times. Which of the statement holds true for such type of cloning?

• A. The restriction site is induced into the vector and the topisomerase enzyme is induced into the PCR primers
• B. The topoismerase I is used for cutting both the strands
• C. The induction of topoisomerase enzyme is done into the vector in the case it is very small in size
• D. The restriction site is induced into the PCR primers and the topoisomerase enzyme is induced into the vector