MCQOPTIONS
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MCQOPTIONS
| 1. |
Mechanism of ligation for both T4 DNA ligase and E. coli DNA ligase makes use of Adenosine Monophosphate (AMP). Which of the steps is involved in the ligation mechanism? |
| A. | AMP is added to the 5’ phosphate of one of the DNA molecule |
| B. | It leads to liberation of pyrophosphate from NAD and nicotinamide mononucleotide from ATP |
| C. | The AMP is further displaced by an electrophilic attack |
| D. | The AMP is further displaced by nucleophilic attack by 3’ hydroxyl of the same DNA molecule |
| Answer» B. It leads to liberation of pyrophosphate from NAD and nicotinamide mononucleotide from ATP | |