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1. |
Hot-start PCR is a modification of PCR. Which of the following is not corresponding to it? |
A. | The basis is that extension is not started until the first cycle reaches its maximum temperature |
B. | The polymerase is added after the first cycle has reached its maximum temperature or melting temperature |
C. | It is satisfactory for small number of samples |
D. | It leads to generation of non specific products |
Answer» E. | |