During amplification, there are chances of having a product of a mixture of different sequences. There are various ways to detect it. Which of the statement is true in regard to it?

• A. Direct sequencing can’t be used in the case if the template DNA is heterozygous at the locus
• B. Direct sequencing can be used if the template DNA is heterozygous at the locus
• C. If cloning is done before sequencing, then it is detected via using only a single clone for sequencing
• D. In the case several recombinants are used, it can’t go undetected