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This section includes 14 Mcqs, each offering curated multiple-choice questions to sharpen your Vector Biology knowledge and support exam preparation. Choose a topic below to get started.
1. |
In which region in the YAC vector is the new DNA inserted in a cloning experiment? |
A. | TEL |
B. | CEN4 |
C. | ORIGIN |
D. | SUP4 |
Answer» E. | |
2. |
What do the sequences TEL signify in a YAC vector? |
A. | Telomeres |
B. | Origins |
C. | Gene center |
D. | Centromere |
Answer» B. Origins | |
3. |
What is the CEN4 region in the YAC vector? |
A. | DNA from centromere |
B. | DNA from telomere |
C. | DNA from origin |
D. | Bacterial DNA |
Answer» B. DNA from telomere | |
4. |
Which of the following is not present in a YAC3 vector? |
A. | TRP1 |
B. | URA3 |
C. | CEN4 |
D. | Amp |
Answer» E. | |
5. |
What is pYAC3? |
A. | Yeast artificial chromosome |
B. | Plasmid vector |
C. | Hybrid of phage and plasmid |
D. | Yeast chromosome |
Answer» B. Plasmid vector | |
6. |
What is an artificial chromosome? |
A. | Isolated from in vivo |
B. | Created in the test tube |
C. | Chromosome of a yeast |
D. | A bacterial chromosome |
Answer» C. Chromosome of a yeast | |
7. |
What are the origins of replication? |
A. | Gene component |
B. | Initiation sites |
C. | Initiation codons |
D. | Stop codons |
Answer» C. Initiation codons | |
8. |
What are telomeres? |
A. | Origin |
B. | Ends |
C. | Chromosomal ends |
D. | Gene component |
Answer» D. Gene component | |
9. |
What are YAC vectors? |
A. | Yeast artificial vectors |
B. | Yeast aggregative vectors |
C. | Yeast artificial chromosomes |
D. | Yeast aggregative chromosomes |
Answer» D. Yeast aggregative chromosomes | |
10. |
YRP recombinants are extremely unstable. |
A. | True |
B. | False |
Answer» B. False | |
11. |
Despite their low copy number, why are YIPs still used? |
A. | Stable recombinants |
B. | Easy availability |
C. | Star activity |
D. | Wider host range |
Answer» B. Easy availability | |
12. |
Why are copy numbers important in a cloning experiment? |
A. | Maximum expression |
B. | Ease of manipulation |
C. | Cost efficiency |
D. | Availability of stock |
Answer» B. Ease of manipulation | |
13. |
Which of the following has the lowest copy number per cell? |
A. | YEPs |
B. | YIPs |
C. | YRPs |
D. | YACs |
Answer» C. YRPs | |
14. |
What is the approximate copy number of Yeast Episomal plasmids? |
A. | 20-50 |
B. | 2-5 |
C. | 100-200 |
D. | 500 |
Answer» B. 2-5 | |