MCQOPTIONS
Saved Bookmarks
MCQOPTIONS
This section includes 13 Mcqs, each offering curated multiple-choice questions to sharpen your Vector Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which of the following selection step does not occur in lac selection system? |
| A. | Ampicillin resistance |
| B. | X-gal plating |
| C. | Agar plating |
| D. | Replica plating |
| Answer» E. | |
| 2. |
What is isopropylthiogalactoside? |
| A. | Plasmid of pUC series |
| B. | E.coli strain |
| C. | Antibiotic |
| D. | Inducer |
| Answer» E. | |
| 3. |
lacZ’ gene present in a Puc series plasmid codes for which of the enzyme? |
| A. | Beta-galactosidase |
| B. | Lactase |
| C. | Amylase |
| D. | Nuclease |
| Answer» B. Lactase | |
| 4. |
WHAT_IS_ISOPROPYLTHIOGALACTOSIDE??$ |
| A. | Plasmid of pUC series |
| B. | E.coli strain |
| C. | Antibiotic |
| D. | Inducer |
| Answer» E. | |
| 5. |
Which_of_the_following_selection_step_does_not_occur_in_lac_selection_system?$ |
| A. | Ampicillin resistance |
| B. | X-gal plating |
| C. | Agar plating |
| D. | Replica plating |
| Answer» E. | |
| 6. |
What is the compound X-gal? |
| A. | Plasmid |
| B. | Lactose analog |
| C. | Antibiotic |
| D. | Inducer |
| Answer» C. Antibiotic | |
| 7. |
In screening for Puc8 recombinants, which color colonies will be the desired recombinants? |
| A. | Blue |
| B. | Colorless |
| C. | White |
| D. | Yellowish |
| Answer» D. Yellowish | |
| 8. |
Beta-galactosidase is one of a series of enzymes involved in the breakdown of lactose to glucose plus galactose. It is normally coded by the gene lacZ, which resides on the E.Coli chromosome. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 9. |
lacZ’ gene present in a Puc series plasmid codes for which of the enzyme?$ |
| A. | Beta-galactosidase |
| B. | Lactase |
| C. | Amylase |
| D. | Nuclease |
| Answer» B. Lactase | |
| 10. |
Which of the following techniques is used in recombinant identification? |
| A. | Ligation |
| B. | Isolation |
| C. | Replica plating |
| D. | Restriction digestion |
| Answer» D. Restriction digestion | |
| 11. |
On a medium containing ampicillin, when pBR322 recombinants were plated. Which of the following will not be able to grow? |
| A. | pBR322 containing gene of interest |
| B. | self-ligated Pbr22 |
| C. | non-transformants |
| D. | transformed cells |
| Answer» D. transformed cells | |
| 12. |
BamHI cuts the Pbr322 at just one position, within the cluster of genes that code for resistance to ___________ |
| A. | Tetracycline |
| B. | Ampicillin |
| C. | Kanamycin |
| D. | Gentamycin |
| Answer» B. Ampicillin | |
| 13. |
Which of the following refers to the inactivation of Insertional inactivation? |
| A. | Host |
| B. | Gene of Interest |
| C. | Gene of the vector |
| D. | Gene of the host |
| Answer» D. Gene of the host | |