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This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Vector Biology knowledge and support exam preparation. Choose a topic below to get started.
1. |
WHICH_OF_THE_FOLLOWING_IS_NOT_A_SOURCE_OF_ALKALINE_PHOSPHATASE_ENZYME??$ |
A. | E.coli |
B. | Calf intestinal tissue |
C. | Arctic shrimp |
D. | Calf thymus tissue |
Answer» E. | |
2. |
Host controlled restriction is a phenomenon related to ________$ |
A. | Bacteria |
B. | Virus |
C. | Plasmid |
D. | Gene of interest |
Answer» B. Virus | |
3. |
The_DNA_to_be_cloned_must_be_cleaved_along_with_the_vector_and_with_the_same_restriction_enzymes.$ |
A. | True |
B. | False |
Answer» B. False | |
4. |
The restriction endonuclease AluI is isolated from which microbe? |
A. | Proteus Vulgaris |
B. | Staphylococcus Aureus |
C. | Arthrobacter Luteus |
D. | Haemophilus Influenzae |
Answer» D. Haemophilus Influenzae | |
5. |
The restriction endonuclease PvuI (isolated from Proteus Vulgaris) cuts DNA at which position? |
A. | Hexanucleotide CGATCG |
B. | Random position |
C. | Towards the end |
D. | Hexanucleotide CAGCTG |
Answer» B. Random position | |
6. |
Which type of restriction endonucleases is used most in genetic engineering? |
A. | Type I |
B. | Type II |
C. | Type III |
D. | Type IV |
Answer» C. Type III | |
7. |
Why_does_the_restriction_phenomenon_in_bacteria_naturally_occur? |
A. | For efficient cloning |
B. | Bacteria produce an enzyme |
C. | Destruction of bacterium’s own DNA |
D. | For survival |
Answer» C. Destruction of bacterium‚Äö√Ñ√∂‚àö√ë‚àö¬•s own DNA | |
8. |
Which of the following statement is not true in case of DNA Polymerase- Reverse transcriptase? |
A. | Involved in the replication of bacteriophage |
B. | Uses RNA as a template |
C. | Used in complementary DNA cloning |
D. | Synthesizes DNA from RNA |
Answer» B. Uses RNA as a template | |
9. |
The Taq DNA polymerase is DNA polymerase _________ enzyme from the bacterium Thermus aquaticus. |
A. | I |
B. | II |
C. | III |
D. | Klenow fragment |
Answer» B. II | |
10. |
Klenow fragment is the modified enzyme of which of the parent DNA polymerase? |
A. | DNA polymerase I |
B. | DNA polymerase II |
C. | DNA polymerase III |
D. | DNA polymerase IV |
Answer» B. DNA polymerase II | |
11. |
Which endonuclease cleaves both single and double stranded DNA molecules, in a non-specific manner? |
A. | S1 |
B. | Bal31 |
C. | DNase I |
D. | BamHI |
Answer» D. BamHI | |
12. |
The enzyme Bal31 purified from the bacterium Alteromonas Espejiana is an example of which enzyme? |
A. | Exonuclease |
B. | Endonuclease |
C. | Ligase |
D. | Phosphatase |
Answer» B. Endonuclease | |
13. |
Enzymes that remove nucleotides one at a time from the end of a DNA molecule are called ____________ |
A. | Ligases |
B. | Exonucleases |
C. | Endonucleases |
D. | Modifying enzymes |
Answer» C. Endonucleases | |
14. |
What type of DNA enzymes is made use of in most of the DNA manipulative techniques? |
A. | Partially degraded |
B. | Purified |
C. | Degraded or denatured |
D. | Enclosed in a parent cell |
Answer» C. Degraded or denatured | |
15. |
Cutting and joining of the DNA are which techniques? |
A. | DNA degradation |
B. | DNA replication |
C. | DNA manipulation |
D. | DNA synthesis |
Answer» D. DNA synthesis | |