MCQOPTIONS
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MCQOPTIONS
This section includes 14 Mcqs, each offering curated multiple-choice questions to sharpen your Vector Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The TGTG motif is located at ______ region. |
| A. | t-10 |
| B. | t-16 |
| C. | t-20 |
| D. | t-25 |
| Answer» C. t-20 | |
| 2. |
In B. subtilis many promoters contain essential ________ motif. |
| A. | tAGAG |
| B. | tTGTG |
| C. | tGGGG |
| D. | tGCGC |
| Answer» C. tGGGG | |
| 3. |
Compared with E.coli, B. subtilis has additional requirements, it can _________ the expression of genes from Gram-negative organisms in ones that are Gram-positive. |
| A. | tInduce |
| B. | tIncrease |
| C. | tPrevent |
| D. | tDecrease |
| Answer» D. tDecrease | |
| 4. |
Poyart and Trie-Cuot constructed a shuttle vector based on PAMB1 for construction of ____________ |
| A. | tTranscriptional fusions |
| B. | tTranslational fusions |
| C. | tBiotherapeutics |
| D. | tFusions |
| Answer» B. tTranslational fusions | |
| 5. |
The original low copy number state of the PAMB1-derived vectors can be restored by _________ of the linker. |
| A. | tInduction |
| B. | tDeletion |
| C. | tDeactivation |
| D. | tRemoval |
| Answer» E. | |
| 6. |
Deactivation of copF gene leads to _________ |
| A. | tIncrease in cell size |
| B. | tIncrease in plasmid copy number |
| C. | tDecrease in cell size |
| D. | tDecrease in plasmid copy number |
| Answer» C. tDecrease in cell size | |
| 7. |
CopF of PAMBI-derived vectors can be deactivated by inserting linker into ____________ |
| A. | tPromoter |
| B. | tInducer |
| C. | tKpnI site |
| D. | tTol site |
| Answer» D. tTol site | |
| 8. |
All the series of cloning vectors developed from PAMB1 carry repE and copF which is a __________ |
| A. | tPromoter |
| B. | tRegulator |
| C. | tGene |
| D. | tProtein |
| Answer» C. tGene | |
| 9. |
Classical E.coli vectors replicate via theta-like structures. |
| A. | tTrue |
| B. | tFalse |
| Answer» B. tFalse | |
| 10. |
Replication of Pamb1 and Ptb19 does not lead to accumulation of _______________ |
| A. | tSingle-stranded DNA |
| B. | tToxins |
| C. | tGenes |
| D. | tNucleases |
| Answer» B. tToxins | |
| 11. |
Plasmid Pamb1 is derived from _____________ |
| A. | tB. subtilis |
| B. | tStreptococcus |
| C. | tYeast |
| D. | tBacillus |
| Answer» C. tYeast | |
| 12. |
Vectors replicating by ____________ mechanism are more stable than those replicating by rolling circle mechanism. |
| A. | tAlpha |
| B. | tTheta |
| C. | tBeta |
| D. | tGamma |
| Answer» C. tBeta | |
| 13. |
____________ DNA is generated during rolling circle mechanism. |
| A. | tDouble stranded |
| B. | tMutated |
| C. | tSingle stranded |
| D. | tWild type |
| Answer» D. tWild type | |
| 14. |
All B. subtilis vectors replicate by _______________ mechanism. |
| A. | tTranslation |
| B. | tMeiosis |
| C. | tMitosis |
| D. | tRolling circle |
| Answer» E. | |