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This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Vector Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
WHICH_OF_THE_FOLLOWING_IS_NOT_A_SOURCE_OF_ALKALINE_PHOSPHATASE_ENZYME??$ |
| A. | E.coli |
| B. | Calf intestinal tissue |
| C. | Arctic shrimp |
| D. | Calf thymus tissue |
| Answer» E. | |
| 2. |
Host controlled restriction is a phenomenon related to ________$ |
| A. | Bacteria |
| B. | Virus |
| C. | Plasmid |
| D. | Gene of interest |
| Answer» B. Virus | |
| 3. |
The_DNA_to_be_cloned_must_be_cleaved_along_with_the_vector_and_with_the_same_restriction_enzymes.$ |
| A. | True |
| B. | False |
| Answer» B. False | |
| 4. |
The restriction endonuclease AluI is isolated from which microbe? |
| A. | Proteus Vulgaris |
| B. | Staphylococcus Aureus |
| C. | Arthrobacter Luteus |
| D. | Haemophilus Influenzae |
| Answer» D. Haemophilus Influenzae | |
| 5. |
The restriction endonuclease PvuI (isolated from Proteus Vulgaris) cuts DNA at which position? |
| A. | Hexanucleotide CGATCG |
| B. | Random position |
| C. | Towards the end |
| D. | Hexanucleotide CAGCTG |
| Answer» B. Random position | |
| 6. |
Which type of restriction endonucleases is used most in genetic engineering? |
| A. | Type I |
| B. | Type II |
| C. | Type III |
| D. | Type IV |
| Answer» C. Type III | |
| 7. |
Why_does_the_restriction_phenomenon_in_bacteria_naturally_occur? |
| A. | For efficient cloning |
| B. | Bacteria produce an enzyme |
| C. | Destruction of bacterium’s own DNA |
| D. | For survival |
| Answer» C. Destruction of bacterium‚Äö√Ñ√∂‚àö√ë‚àö¬•s own DNA | |
| 8. |
Which of the following statement is not true in case of DNA Polymerase- Reverse transcriptase? |
| A. | Involved in the replication of bacteriophage |
| B. | Uses RNA as a template |
| C. | Used in complementary DNA cloning |
| D. | Synthesizes DNA from RNA |
| Answer» B. Uses RNA as a template | |
| 9. |
The Taq DNA polymerase is DNA polymerase _________ enzyme from the bacterium Thermus aquaticus. |
| A. | I |
| B. | II |
| C. | III |
| D. | Klenow fragment |
| Answer» B. II | |
| 10. |
Klenow fragment is the modified enzyme of which of the parent DNA polymerase? |
| A. | DNA polymerase I |
| B. | DNA polymerase II |
| C. | DNA polymerase III |
| D. | DNA polymerase IV |
| Answer» B. DNA polymerase II | |
| 11. |
Which endonuclease cleaves both single and double stranded DNA molecules, in a non-specific manner? |
| A. | S1 |
| B. | Bal31 |
| C. | DNase I |
| D. | BamHI |
| Answer» D. BamHI | |
| 12. |
The enzyme Bal31 purified from the bacterium Alteromonas Espejiana is an example of which enzyme? |
| A. | Exonuclease |
| B. | Endonuclease |
| C. | Ligase |
| D. | Phosphatase |
| Answer» B. Endonuclease | |
| 13. |
Enzymes that remove nucleotides one at a time from the end of a DNA molecule are called ____________ |
| A. | Ligases |
| B. | Exonucleases |
| C. | Endonucleases |
| D. | Modifying enzymes |
| Answer» C. Endonucleases | |
| 14. |
What type of DNA enzymes is made use of in most of the DNA manipulative techniques? |
| A. | Partially degraded |
| B. | Purified |
| C. | Degraded or denatured |
| D. | Enclosed in a parent cell |
| Answer» C. Degraded or denatured | |
| 15. |
Cutting and joining of the DNA are which techniques? |
| A. | DNA degradation |
| B. | DNA replication |
| C. | DNA manipulation |
| D. | DNA synthesis |
| Answer» D. DNA synthesis | |