Explore topic-wise MCQs in Biotechnology.

This section includes 51 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.

1.

Two genes from a bacterium () and a cotton gene produce

A. a plastic
B. a vaccine against the bacterium
C. a pharmaceutical product
D. herbicide resistance
Answer» B. a vaccine against the bacterium
Discussion
2.

Plants derived sexually from the same plant are____ while those derived from somatic tissue from the same plant are____.
A. identical, different
B. different, also different
C. different, identical
D. plants cannot be derived from somatic tissue
Answer» D. plants cannot be derived from somatic tissue
Discussion
3.

Transgenic plants with increased tolerance to aluminum have been produced by making plants that
A. secrete phytosiderophores into the soil
B. make more metal-binding peptides like phytochelatins
C. bind aluminum to the cell wall
D. secrete citrate into the soil
Answer» E.
Discussion
4.

Plants containing genes encoding cytokines and blood clotting factors are used in
A. nutrition improvement
B. pharmaceutical production
C. vaccine production
D. textile production
Answer» C. vaccine production
Discussion
5.

The first transgenic plants expressing engineered foreign genes were tobacco plants produced by the use of
A. <i>Agrobacterium tumefaciens</i>
B. <i>Bacillus thuringiensis</i>
C. <i>Arabidopsis thaliana</i>
D. <i>Streptomyces hygroscopicus</i>
Answer» B. <i>Bacillus thuringiensis</i>
Discussion
6.

Which of the agricultural challenges below cannot be solved with transgenic techniques?
A. Crops are damaged by frost
B. Crops are killed by a virus
C. Public concern about safety of synthetic pesticides
D. Public preference for organic vegetables
Answer» E.
Discussion
7.

Transplastomics
A. targets genes in the chloroplast
B. provides exceptionally low yields of protein products
C. produces genes that are released in pollen
D. offers little opportunity for practical use
Answer» B. provides exceptionally low yields of protein products
Discussion
8.

Which of the following statements is true regarding genomics?
A. Plant genomics lags behind similar efforts in animals and microorganisms
B. Researchers can make use of genomic information even if the entire genome of an organism is not known
C. Researchers are busy in trying to determine the genome of the potato plant
D. All of the above
Answer» E.
Discussion
9.

Which of the following gene is responsible for resistance against chilling?
A. Glycerol 1 phosphate acyl transferase
B. Polygalactouranase
C. ACC deaminase
D. Sucrose phosphate synthase gene
Answer» B. Polygalactouranase
Discussion
10.

Which of the following gene detoxifies herbicide phosphinothricin?
A. Nitrilase
B. Glutathione S-transferase (GST)
C. Phosphinothricin acetyl transferase
D. All of these
Answer» D. All of these
Discussion
11.

The controversy regarding the use of Bt corn is that it
A. is potentially harmful to monarch butterflies
B. is a potential allergen to humans
C. both (a) and (b)
D. can contaminate groundwater
Answer» D. can contaminate groundwater
Discussion
12.

Starch content of potatoes can be increased by using a bacterial gene, known as
A. sucrose phosphate synthase gene
B. ADP glucose pyrophosphorylase gene
C. polygalactouranase gene
D. none of the above
Answer» C. polygalactouranase gene
Discussion
13.

In a __________ protocol, bacteria with engineered abilities to detoxify pollutants are intentionally released in an area.
A. microcosm establishment
B. mibridization
C. bioremediation
D. rhizosecretion
Answer» D. rhizosecretion
Discussion
14.

Resistance to sulphonyl urea compounds in transgenic tobacco plant has been developed by the transfer of
A. gene for EPSPS (5-enol-pyruvyl shikimat 3 phosphate synthase)
B. gene for ALS (acetolactate synthase)
C. gene for GS (glutamine synthase)
D. any of the above
Answer» C. gene for GS (glutamine synthase)
Discussion
15.

Resistance to glyphosphate in transgenic petunia plants has been developed by the transfer of
A. gene for EPSPS(5-enol-pyruvyl shikimat 3 phosphate synthase)
B. gene for ALS (acetolactate synthase)
C. gene for GS (glutamine synthase)
D. any of the above
Answer» B. gene for ALS (acetolactate synthase)
Discussion
16.

Which of the following compounds has been produced in transgenic plants to improve tolerance to salt stress and water deficit?
A. Sucrose
B. Mannitol
C. Nicotine
D. Octopine
Answer» C. Nicotine
Discussion
17.

Expression of antisense RNA in transgenic plants is a general method used to
A. activate the expression of all genes in a biochemical pathway
B. eliminate the expression of all genes in a biochemical pathway
C. block the expression of virus coat protein genes
D. reduce or eliminate the expression of individual genes
Answer» E.
Discussion
18.

Resistance to L-phosphinothrium in tobacco plants is developed by the transfer of
A. gene for EPSPS (5-enol-pyruvyl shikimat 3 phosphate synthase)
B. gene for ALS (acetolactate synthase)
C. gene for GS (glutamine synthase)
D. any of the above
Answer» D. any of the above
Discussion
19.

A __________ is a small laboratory set up to simulate conditions of a particular environment.
A. microcosm
B. mibridization
C. bioremediation
D. rhizosecretion
Answer» B. mibridization
Discussion
20.

The roots of a plant are converted into drug-producing structures in a process called
A. microcosm establishment
B. mibridization
C. bioremediation
D. rhizosecretion
Answer» E.
Discussion
21.

Which cell-based technology endows a cell with increased ability to harness energy?
A. Chlybridization
B. Cybridization
C. Mibridization
D. Protoplast fusion
Answer» B. Cybridization
Discussion
22.

The delayed ripening tomato was created by a biotechnologist who __________ a gene.
A. altered
B. silenced
C. replaced
D. relocated
Answer» C. replaced
Discussion
23.

Antisense technology
A. selectively blocks expression of a gene
B. combines genetic material from different species
C. combines organelles and cells
D. alters or transfers cells
Answer» B. combines genetic material from different species
Discussion
24.

Antisense transgenic plants produced fruit that softened
A. more slowly than the normal fruit
B. more rapidly than the normal fruit
C. as the normal fruits
D. none of these
Answer» B. more rapidly than the normal fruit
Discussion
25.

ACC demainase gene is responsible for
A. synthesis for ethylene
B. degradation of ACC an immediate precursor to ethylene
C. both (a) and (b)
D. synthesis of polygalactouranase
Answer» C. both (a) and (b)
Discussion
26.

Which of the following genes can be used for making resistances against viral infection?
A. Genes for capsid protein
B. Gene for nucleocapsid protein
C. Satellite RNA
D. All of these
Answer» E.
Discussion
27.

Cross protection against viruses in transgenic plants can be obtained by
A. inoculating the susceptible strain of a crop with a mild strain of a virus that helps in developing resistance against more virulent strain
B. inoculating the susceptible strain with the coat proteins of virulent strain
C. inoculating the susceptible strain with genes of nucleocapsid
D. any of the above
Answer» B. inoculating the susceptible strain with the coat proteins of virulent strain
Discussion
28.

The first field tests were conducted with which of the following genetically altered organism?
A. bt Corn
B. Vaccinia virus containing a gene from the rabies virus
C. The flavrsavr tomato
D. Strawberry seedlings sprayed with ice-minus bacteria
Answer» E.
Discussion
29.

Which of the following self-pollinating plant/(s) tend to be homozygous?
A. Peas
B. Tomato
C. Peach
D. All of these
Answer» E.
Discussion
30.

Which cell-based plant technology involves the combining of two cells without cell walls from different species?
A. Clonal propagation
B. Cybridization
C. Protoplast fusion
D. Mutant selection
Answer» D. Mutant selection
Discussion
31.

A naturally occurring variant, possessing characteristics of interest, is identified. This plant is selectively bred. This is an example of
A. traditional plant breeding
B. transgenic technology
C. mutant selection
D. none of these
Answer» B. transgenic technology
Discussion
32.

Transgenic plants
A. contain foreign genes in their cells
B. are used to produce human antibodies
C. both (a) and (b)
D. are plants that differ in geographical locations
Answer» D. are plants that differ in geographical locations
Discussion
33.

Low temperatures induce the expression of many cold-induced genes. Transgenic plants with improved cold tolerance have been produced by
A. expressing the protein that activates expression of these genes all the time in plants
B. cooling plants using the Peltier effect
C. expressing a gene for production of antifreeze (ethylene glycol) in plants
D. increasing evaporative cooling from leaf surfaces
Answer» B. cooling plants using the Peltier effect
Discussion
34.

Given the DNA sequence ATTAAGGC, which sequence would be used by researchers as an antisense molecule?
A. AUUAAGGC
B. UAAUUCCG
C. CGGAATTA
D. TAATTCCG
Answer» B. UAAUUCCG
Discussion
35.

If the goal were to create a plant resistant to an insecticide, which cell-based plant technology would be most effective?
A. Clonal propagation
B. Cybridization
C. Protoplast fusion
D. Mutant selection
Answer» E.
Discussion
36.

What are the various disadvantages of cross protection?
A. Possibility of mutations in inducing mild virus strain
B. Possibility of synergism between inducing virus and other unrelated virus
C. Possibility of unnecessary spread of mild virus
D. All of the above
Answer» E.
Discussion
37.

In transgenic plants that have been manipulated to express patho-genesis-related (PR) proteins all the time it has been shown that
A. these provide no protection at all against pathogens
B. expression of one PR protein provides high levels of protection against many pathogens
C. expression of two PR proteins provides some protection against a few pathogens
D. these proteins trigger the production of other antimicrobial defenses
Answer» E.
Discussion
38.

Which of the following gene detoxify herbicide bronoxynil?
A. Nitrilase
B. Glutathione S-transferase (GST)
C. Phosphinothricin acetyl transferase
D. All of these
Answer» B. Glutathione S-transferase (GST)
Discussion
39.

For developing herbicide resistance in transgenic plants which of the following approach/(es) is/are used?
A. Target molecule is made insensitive to herbicide
B. Target protein is overproduced
C. A pathway should be introduced that detoxify the herbicide
D. All of the above
Answer» E.
Discussion
40.

Which of the following is not a characteristic of a transgenic crop?
A. herbicide resistance
B. Bt insect resistance toxin
C. increased methionine content
D. none of these
Answer» E.
Discussion
41.

Reducing the expression of pectin methylesterase (PME) has resulted in
A. plants with resistance to bacteria and fungi
B. tomato juice that has higher viscosity
C. tomato fruit that last longer before they become rotten
D. tomato fruit that will not ripen
Answer» C. tomato fruit that last longer before they become rotten
Discussion
42.

Which of the following gene is transferred to plants that detoxify the herbicide atrazine?
A. Nitrilase
B. Glutathione S-transferase (GST)
C. Phosphinothrium acetyl transferase
D. All of these
Answer» C. Phosphinothrium acetyl transferase
Discussion
43.

Two genes from a bacterium (Alcaligenes eutrophus) and a cotton gene produce
A. a plastic
B. a vaccine against the bacterium
C. a pharmaceutical product
D. herbicide resistance
Answer» B. a vaccine against the bacterium
Discussion
44.

Bruise resistant tomatoes have been developed by the expression of antisense RNA against
A. glycerol 1 phosphate acyl transferase
B. polygalactouranase
C. ACC deaminase
D. Sucrose phosphate synthase gene
Answer» C. ACC deaminase
Discussion
45.

Which of the following has been widely used to provide resistance against plant viruses?
A. Virus resistance genes from bacteria
B. Expression of virus coat protein genes in transgenic plants
C. Expression of anti-virus genes in vectors that transmit viruses
D. Expression of ribonuclease (RNase) genes in host plants
Answer» C. Expression of anti-virus genes in vectors that transmit viruses
Discussion
46.

Insect resistance in the transgenic plant has been achieved by
A. transferring genes for Bt toxins
B. transferring genes for protease inhibitors
C. transferring genes for other insecticidal secondary metabolities
D. all of the above
Answer» E.
Discussion
47.

Metabolic interference is a term used to describe a method to metabolize a compound and prevent the synthesis of something that is normally produced. What compound(s) have been targeted for metabolic interference in tomato?
A. ACC (1-aminocyclopropane-1-carboxylic acid)
B. SAM (S-adenosylmethionine)
C. Both (a) and (b)
D. AOA (aminooxyacetic acid)
Answer» D. AOA (aminooxyacetic acid)
Discussion
48.

Which of the following metabolites are implicated in stress tolerance?
A. Proline
B. Betaines
C. Both (a) and (b)
D. Citrate
Answer» D. Citrate
Discussion
49.

Which of the following dies from Ti plasmid infection?
A. Rice
B. Corn
C. Sorghum
D. All of these
Answer» E.
Discussion
50.

Tomatoes exhibiting delayed ripening express antisense RNA against
A. glycerol 1 phosphate acyl transferase
B. polygalactouranase
C. ACC deaminase
D. sucrose phosphate synthase gene
Answer» D. sucrose phosphate synthase gene
Discussion