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This section includes 87 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.
1. |
If complex III were incorporated into an artificial lipid vesicle in order to demonstrate it's proton translocating ability in isolation, which of the following would be an appropriate electron donor? |
A. | Cytochrome c red |
B. | Cytochrome c peroxidase |
C. | Ubiquinol (UQH2) |
D. | Cytochrome c oxidase |
Answer» D. Cytochrome c oxidase | |
2. |
Which of the following experimental results does not support Mitchell's chemiosmosis theory? |
A. | Electron transport in isolated mitochondria was shown to result in acidification of the medium |
B. | Addition of dinitrophenol to isolated mitochondria was shown to inhibit electron transport, but had no effect on |
C. | synthesis |
D. | Addition of dinitrophenol to isolated mitochondria during electron transport was shown to inhibit acidification of the medium |
E. | An artificial proton gradient across the inner mitochondrial membrane drives ATP synthesis in the absence of electron transport |
Answer» C. synthesis | |
3. |
"The universe tends towards maximum disorder" is stated by |
A. | first law of thermodynamics |
B. | second law of thermodynamics |
C. | third law of thermodynamics |
D. | none of the above |
Answer» C. third law of thermodynamics | |
4. |
If the ester bonds of a triacylglycerol molecule's are broken then the kind of molecule, which would be soluble in water, is |
A. | fatty acid |
B. | steroid |
C. | glycogen |
D. | glycerol |
Answer» E. | |
5. |
Agrobacterium based gene transfer is efficient |
A. | only with dicots |
B. | only with monocots |
C. | with both monocots and dicots |
D. | with majority monocots and few dicots |
Answer» B. only with monocots | |
6. |
Which method of plant propagation involves the use of girdling? |
A. | Grafting |
B. | Cuttings |
C. | Layering |
D. | Micropropagation |
Answer» D. Micropropagation | |
7. |
Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation? |
A. | Multiplication of sexually derived sterile hybrids |
B. | Less multiplication of disease free plants |
C. | Storage and transportation of propogates |
D. | Both (b) and (c) |
Answer» D. Both (b) and (c) | |
8. |
Which of the following is not true about nurse or conditioned medium? |
A. | It is liquid removed from the suspension of fast growing cells |
B. | It contains uncharacterized growth factor released by growing cells |
C. | It is used in the culture of regenerating protoplast |
D. | it is removed aseptically from the culture and is autoclaved before use |
Answer» E. | |
9. |
When plated only in nutrient medium, how much time is required for the protoplast to synthesize new cell wall? |
A. | 2-5 days |
B. | 5-10 days |
C. | 10-15 days |
D. | 15-17 days |
Answer» C. 10-15 days | |
10. |
The ability of the component cells of callus to form a whole plant is known as |
A. | redifferentiation |
B. | dedifferentiation |
C. | either (a) or (b) |
D. | none of these |
Answer» B. dedifferentiation | |
11. |
Which of the following is not a cytokinin? |
A. | 2,4-dichlorophenoxyacetic acid |
B. | 6 benzylaminopurine |
C. | Zeatin |
D. | Kinetin |
Answer» B. 6 benzylaminopurine | |
12. |
Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media |
A. | utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise |
B. | utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise |
C. | utilization of both ammonium and nitrate result in rise in pH |
D. | utilization of both ammonium and nitrate result in drop in pH |
Answer» B. utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise | |
13. |
Which breeding method uses a chemical to strip the cell wall of plant cells of two sexually incompatible species? |
A. | Mass selection |
B. | Protoplast fusion |
C. | Transformation |
D. | Transpiration |
Answer» C. Transformation | |
14. |
In a callus culture |
A. | increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation |
B. | increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation |
C. | auxins and cytokinins are not required |
D. | only auxin is required for root and shoot formation |
Answer» B. increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation | |
15. |
When guard cells swell |
A. | stomata close |
B. | stomata open |
C. | photosynthesis stops |
D. | evaporation stops |
Answer» C. photosynthesis stops | |
16. |
The evaporation of water from leaves is called |
A. | transpiration |
B. | cohesion |
C. | tension |
D. | pressure flow |
Answer» B. cohesion | |
17. |
Which of the following statements about the outer mitochondrial membrane is not true? |
A. | The outer membrane is impermeable to hydrogen ions |
B. | The outer membrane is highly permeable to substances of small molecular weight |
C. | The outer membrane is about 50% lipid and 50% protein by weight |
D. | The outer membrane contains porins |
Answer» B. The outer membrane is highly permeable to substances of small molecular weight | |
18. |
The cell membrane is made up of |
A. | lipoproteins |
B. | cellulose |
C. | phospholipids |
D. | phosphoproteins |
Answer» B. cellulose | |
19. |
Cellular totipotency is the property of |
A. | plants |
B. | animals |
C. | bacteria |
D. | all of these |
Answer» B. animals | |
20. |
Which is/are the naturally occurring plant auxins? |
A. | Indole acetic acid (IAA) |
B. | Naphthalenacetic acid (NAA) |
C. | 2,4-dichlorophenoxyacetic acid |
D. | All of the above |
Answer» B. Naphthalenacetic acid (NAA) | |
21. |
Which of the following growth regulator promote cell division? |
A. | Auxins |
B. | Cytokinins |
C. | Gibberellins |
D. | Brassinosteroids |
Answer» C. Gibberellins | |
22. |
If complex III were incorporated into an artificial lipid vesicle in order to demonstrate it's proton translocating ability in isolation, which of the following would be an appropriate electron donor? |
A. | Cytochrome c red |
B. | Cytochrome c peroxidase |
C. | Ubiquinol (UQH2) |
D. | Cytochrome c oxidase |
Answer» D. Cytochrome c oxidase | |
23. |
A(n) __________ is an excised piece of leaf or stem tissue used in micropropagation. |
A. | microshoot |
B. | medium |
C. | explant |
D. | scion |
Answer» D. scion | |
24. |
What is/are the benefit(s) of micropropagation or clonal propagation? |
A. | Rapid multiplication of superior clones |
B. | Multiplication of disease free plants |
C. | Multiplication of sexually derived sterile hybrids |
D. | All of the above |
Answer» E. | |
25. |
If the ester bonds of a triacylglycerol molecule's are broken then the kind of molecule, which would be soluble in water, is |
A. | fatty acid |
B. | steroid |
C. | glycogen |
D. | glycerol |
Answer» E. | |
26. |
Cellular organelles are embedded in the |
A. | endoplasmic reticulum |
B. | cristae |
C. | cytosol |
D. | microtubules |
Answer» D. microtubules | |
27. |
The cellular organelle associated with photosynthesis is the |
A. | golgi complex |
B. | mitochondrion |
C. | lysosome |
D. | chloroplast |
Answer» E. | |
28. |
A cell without cell wall is called |
A. | apoplast |
B. | protoplast |
C. | symplast |
D. | none of these |
Answer» C. symplast | |
29. |
Which cell type is likely to have the most mitochondria? |
A. | Fast-twitch muscle |
B. | Slow-twitch muscle |
C. | Liver cells |
D. | Adipose cells |
Answer» C. Liver cells | |
30. |
Tonoplast is a membrane around |
A. | cytoplasm |
B. | vacuole |
C. | nucleus |
D. | mitochondria |
Answer» C. nucleus | |
31. |
Which part of a plant contains the male sex cell? |
A. | Ova |
B. | Style |
C. | Stamen |
D. | Pollen |
Answer» E. | |
32. |
Chemical energy is supplied to eukaryotic cells by |
A. | chloroplasts and golgi complexes |
B. | mitochondria and ribosomes |
C. | mitochondria only |
D. | mitochondria and chloroplasts |
Answer» E. | |
33. |
The movement of most minerals, including potassium into root cells is by |
A. | osmosis |
B. | diffusion |
C. | bulk flow |
D. | active transport |
Answer» E. | |
34. |
What are the macronutrients used in plant cell culture medium? |
A. | N, P, K, S, Na |
B. | N, P, K, Ca, Cl |
C. | N, P, K, S, Ca |
D. | N, P, Ca, Na, Cl |
Answer» D. N, P, Ca, Na, Cl | |
35. |
Which of the following growth regulator is added for short initiation during plant regeneration from callus? |
A. | Auxins |
B. | Cytokinins |
C. | Gibberellins |
D. | Brassinosteroids |
Answer» C. Gibberellins | |
36. |
The cohesion-tension theory of water movement helps to explain how water moves from soil into root? |
A. | Through the phloem |
B. | From leaf to root |
C. | Through the xylem |
D. | Into the guard cells |
Answer» D. Into the guard cells | |
37. |
Which of the following growth regulator cause plant cells to grow? |
A. | Auxins |
B. | Cytokinins |
C. | Gibberellins |
D. | Brassinosteroids |
Answer» B. Cytokinins | |
38. |
Which of the following observations is not used as evidence to support the concept of endosymbiotic origin of chloroplasts and mitochondria? |
A. | Both have their own DNA |
B. | Both have their own ribosomes |
C. | Both strongly resemble prokaryotes, especially in size |
D. | Both can live and function independently of the cell |
Answer» E. | |
39. |
Mitochondria can be expected most numerous in |
A. | green cells |
B. | red blood cells |
C. | muscle cells |
D. | none of these |
Answer» D. none of these | |
40. |
The element neon (Ne) has eight electrons in its outermost electron shell. How many covalent bonds will Ne readily form? |
A. | None |
B. | One |
C. | Two |
D. | Four |
Answer» B. One | |
41. |
The cellular organelle associated with secretion is the |
A. | golgi complex |
B. | mitochondrion |
C. | ribosome |
D. | lysosome |
Answer» B. mitochondrion | |
42. |
A widely accepted explanation for transport of sugar in phloem is called |
A. | root pressure theory |
B. | transpiration |
C. | pressure flow hypothesis |
D. | cytoplasmic streaming |
Answer» D. cytoplasmic streaming | |
43. |
The fluid matrix of a cell is called the |
A. | nucleus |
B. | cytosol |
C. | cytoskeleton |
D. | endoplasmic reticulum |
Answer» C. cytoskeleton | |
44. |
Specialized type of peroxisome and the site of glyoxylate pathway in certain plants is known as |
A. | gly-peroxisome |
B. | lysosome |
C. | glyoxysome |
D. | none of these |
Answer» D. none of these | |
45. |
Which is the most common carbon source used in the plant cell culture media? |
A. | Sucrose |
B. | Glucose |
C. | Fructose |
D. | Maltose |
Answer» B. Glucose | |
46. |
Any system that is not at equilibrium |
A. | is thermodynamically unstable, although it may be kinetically stable |
B. | is kinetically unstable, although it may be thermodynamically stable |
C. | is rushing toward equilibrium at a very rapid rate |
D. | requires an enzyme to reach equilibrium |
Answer» B. is kinetically unstable, although it may be thermodynamically stable | |
47. |
Neutralized activated charcoal is occasionally added to young regenerating cultures to |
A. | remove toxic phenolics produced by the stressed plant cell |
B. | help to remove plants growth regulators introduced at an earlier stage |
C. | both (a) and (b) |
D. | maintain the pH of the medium |
Answer» D. maintain the pH of the medium | |
48. |
Which molecule serves as a carrier of fatty acids in mitochondria? |
A. | Acetyl-CoA |
B. | Carnitine |
C. | Dolichol |
D. | Malonyl-CoA |
Answer» C. Dolichol | |
49. |
Phloem is composed of |
A. | vessel elements and companion cells |
B. | tracheids and vessel elements |
C. | sieve-tube elements and companion cells |
D. | tracheids and sieve-tube elements |
Answer» D. tracheids and sieve-tube elements | |
50. |
Water moves into roots of plants by |
A. | osmosis |
B. | active transport |
C. | diffusion |
D. | pressure flow mechanism |
Answer» B. active transport | |