Explore topic-wise MCQs in Biotechnology.

This section includes 87 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.

1.

If complex III were incorporated into an artificial lipid vesicle in order to demonstrate it's proton translocating ability in isolation, which of the following would be an appropriate electron donor?

A. Cytochrome c red
B. Cytochrome c peroxidase
C. Ubiquinol (UQH2)
D. Cytochrome c oxidase
Answer» D. Cytochrome c oxidase
2.

Which of the following experimental results does not support Mitchell's chemiosmosis theory?

A. Electron transport in isolated mitochondria was shown to result in acidification of the medium
B. Addition of dinitrophenol to isolated mitochondria was shown to inhibit electron transport, but had no effect on
C. synthesis
D. Addition of dinitrophenol to isolated mitochondria during electron transport was shown to inhibit acidification of the medium
E. An artificial proton gradient across the inner mitochondrial membrane drives ATP synthesis in the absence of electron transport
Answer» C. synthesis
3.

"The universe tends towards maximum disorder" is stated by

A. first law of thermodynamics
B. second law of thermodynamics
C. third law of thermodynamics
D. none of the above
Answer» C. third law of thermodynamics
4.

If the ester bonds of a triacylglycerol molecule's are broken then the kind of molecule, which would be soluble in water, is

A. fatty acid
B. steroid
C. glycogen
D. glycerol
Answer» E.
5.

Agrobacterium based gene transfer is efficient

A. only with dicots
B. only with monocots
C. with both monocots and dicots
D. with majority monocots and few dicots
Answer» B. only with monocots
6.

Which method of plant propagation involves the use of girdling?

A. Grafting
B. Cuttings
C. Layering
D. Micropropagation
Answer» D. Micropropagation
7.

Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation?

A. Multiplication of sexually derived sterile hybrids
B. Less multiplication of disease free plants
C. Storage and transportation of propogates
D. Both (b) and (c)
Answer» D. Both (b) and (c)
8.

Which of the following is not true about nurse or conditioned medium?

A. It is liquid removed from the suspension of fast growing cells
B. It contains uncharacterized growth factor released by growing cells
C. It is used in the culture of regenerating protoplast
D. it is removed aseptically from the culture and is autoclaved before use
Answer» E.
9.

When plated only in nutrient medium, how much time is required for the protoplast to synthesize new cell wall?

A. 2-5 days
B. 5-10 days
C. 10-15 days
D. 15-17 days
Answer» C. 10-15 days
10.

The ability of the component cells of callus to form a whole plant is known as

A. redifferentiation
B. dedifferentiation
C. either (a) or (b)
D. none of these
Answer» B. dedifferentiation
11.

Which of the following is not a cytokinin?

A. 2,4-dichlorophenoxyacetic acid
B. 6 benzylaminopurine
C. Zeatin
D. Kinetin
Answer» B. 6 benzylaminopurine
12.

Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media

A. utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise
B. utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise
C. utilization of both ammonium and nitrate result in rise in pH
D. utilization of both ammonium and nitrate result in drop in pH
Answer» B. utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise
13.

Which breeding method uses a chemical to strip the cell wall of plant cells of two sexually incompatible species?

A. Mass selection
B. Protoplast fusion
C. Transformation
D. Transpiration
Answer» C. Transformation
14.

In a callus culture

A. increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
B. increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation
C. auxins and cytokinins are not required
D. only auxin is required for root and shoot formation
Answer» B. increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation
15.

When guard cells swell

A. stomata close
B. stomata open
C. photosynthesis stops
D. evaporation stops
Answer» C. photosynthesis stops
16.

The evaporation of water from leaves is called

A. transpiration
B. cohesion
C. tension
D. pressure flow
Answer» B. cohesion
17.

Which of the following statements about the outer mitochondrial membrane is not true?

A. The outer membrane is impermeable to hydrogen ions
B. The outer membrane is highly permeable to substances of small molecular weight
C. The outer membrane is about 50% lipid and 50% protein by weight
D. The outer membrane contains porins
Answer» B. The outer membrane is highly permeable to substances of small molecular weight
18.

The cell membrane is made up of

A. lipoproteins
B. cellulose
C. phospholipids
D. phosphoproteins
Answer» B. cellulose
19.

Cellular totipotency is the property of

A. plants
B. animals
C. bacteria
D. all of these
Answer» B. animals
20.

Which is/are the naturally occurring plant auxins?

A. Indole acetic acid (IAA)
B. Naphthalenacetic acid (NAA)
C. 2,4-dichlorophenoxyacetic acid
D. All of the above
Answer» B. Naphthalenacetic acid (NAA)
21.

Which of the following growth regulator promote cell division?

A. Auxins
B. Cytokinins
C. Gibberellins
D. Brassinosteroids
Answer» C. Gibberellins
22.

If complex III were incorporated into an artificial lipid vesicle in order to demonstrate it's proton translocating ability in isolation, which of the following would be an appropriate electron donor?

A. Cytochrome c red
B. Cytochrome c peroxidase
C. Ubiquinol (UQH2)
D. Cytochrome c oxidase
Answer» D. Cytochrome c oxidase
23.

A(n) __________ is an excised piece of leaf or stem tissue used in micropropagation.

A. microshoot
B. medium
C. explant
D. scion
Answer» D. scion
24.

What is/are the benefit(s) of micropropagation or clonal propagation?

A. Rapid multiplication of superior clones
B. Multiplication of disease free plants
C. Multiplication of sexually derived sterile hybrids
D. All of the above
Answer» E.
25.

If the ester bonds of a triacylglycerol molecule's are broken then the kind of molecule, which would be soluble in water, is

A. fatty acid
B. steroid
C. glycogen
D. glycerol
Answer» E.
26.

Cellular organelles are embedded in the

A. endoplasmic reticulum
B. cristae
C. cytosol
D. microtubules
Answer» D. microtubules
27.

The cellular organelle associated with photosynthesis is the

A. golgi complex
B. mitochondrion
C. lysosome
D. chloroplast
Answer» E.
28.

A cell without cell wall is called

A. apoplast
B. protoplast
C. symplast
D. none of these
Answer» C. symplast
29.

Which cell type is likely to have the most mitochondria?

A. Fast-twitch muscle
B. Slow-twitch muscle
C. Liver cells
D. Adipose cells
Answer» C. Liver cells
30.

Tonoplast is a membrane around

A. cytoplasm
B. vacuole
C. nucleus
D. mitochondria
Answer» C. nucleus
31.

Which part of a plant contains the male sex cell?

A. Ova
B. Style
C. Stamen
D. Pollen
Answer» E.
32.

Chemical energy is supplied to eukaryotic cells by

A. chloroplasts and golgi complexes
B. mitochondria and ribosomes
C. mitochondria only
D. mitochondria and chloroplasts
Answer» E.
33.

The movement of most minerals, including potassium into root cells is by

A. osmosis
B. diffusion
C. bulk flow
D. active transport
Answer» E.
34.

What are the macronutrients used in plant cell culture medium?

A. N, P, K, S, Na
B. N, P, K, Ca, Cl
C. N, P, K, S, Ca
D. N, P, Ca, Na, Cl
Answer» D. N, P, Ca, Na, Cl
35.

Which of the following growth regulator is added for short initiation during plant regeneration from callus?

A. Auxins
B. Cytokinins
C. Gibberellins
D. Brassinosteroids
Answer» C. Gibberellins
36.

The cohesion-tension theory of water movement helps to explain how water moves from soil into root?

A. Through the phloem
B. From leaf to root
C. Through the xylem
D. Into the guard cells
Answer» D. Into the guard cells
37.

Which of the following growth regulator cause plant cells to grow?

A. Auxins
B. Cytokinins
C. Gibberellins
D. Brassinosteroids
Answer» B. Cytokinins
38.

Which of the following observations is not used as evidence to support the concept of endosymbiotic origin of chloroplasts and mitochondria?

A. Both have their own DNA
B. Both have their own ribosomes
C. Both strongly resemble prokaryotes, especially in size
D. Both can live and function independently of the cell
Answer» E.
39.

Mitochondria can be expected most numerous in

A. green cells
B. red blood cells
C. muscle cells
D. none of these
Answer» D. none of these
40.

The element neon (Ne) has eight electrons in its outermost electron shell. How many covalent bonds will Ne readily form?

A. None
B. One
C. Two
D. Four
Answer» B. One
41.

The cellular organelle associated with secretion is the

A. golgi complex
B. mitochondrion
C. ribosome
D. lysosome
Answer» B. mitochondrion
42.

A widely accepted explanation for transport of sugar in phloem is called

A. root pressure theory
B. transpiration
C. pressure flow hypothesis
D. cytoplasmic streaming
Answer» D. cytoplasmic streaming
43.

The fluid matrix of a cell is called the

A. nucleus
B. cytosol
C. cytoskeleton
D. endoplasmic reticulum
Answer» C. cytoskeleton
44.

Specialized type of peroxisome and the site of glyoxylate pathway in certain plants is known as

A. gly-peroxisome
B. lysosome
C. glyoxysome
D. none of these
Answer» D. none of these
45.

Which is the most common carbon source used in the plant cell culture media?

A. Sucrose
B. Glucose
C. Fructose
D. Maltose
Answer» B. Glucose
46.

Any system that is not at equilibrium

A. is thermodynamically unstable, although it may be kinetically stable
B. is kinetically unstable, although it may be thermodynamically stable
C. is rushing toward equilibrium at a very rapid rate
D. requires an enzyme to reach equilibrium
Answer» B. is kinetically unstable, although it may be thermodynamically stable
47.

Neutralized activated charcoal is occasionally added to young regenerating cultures to

A. remove toxic phenolics produced by the stressed plant cell
B. help to remove plants growth regulators introduced at an earlier stage
C. both (a) and (b)
D. maintain the pH of the medium
Answer» D. maintain the pH of the medium
48.

Which molecule serves as a carrier of fatty acids in mitochondria?

A. Acetyl-CoA
B. Carnitine
C. Dolichol
D. Malonyl-CoA
Answer» C. Dolichol
49.

Phloem is composed of

A. vessel elements and companion cells
B. tracheids and vessel elements
C. sieve-tube elements and companion cells
D. tracheids and sieve-tube elements
Answer» D. tracheids and sieve-tube elements
50.

Water moves into roots of plants by

A. osmosis
B. active transport
C. diffusion
D. pressure flow mechanism
Answer» B. active transport