In an experiment, you wanted to see which mutation will have worse effect on transcription. In the 1st set you deleted 94 amino acids from the C-terminus and in the 2nd set you replace the 265th C-terminal arginine to cystine. Which in your opinion will have the worst effect?

No visible effect on rate of transcription

Both will have similar negative effect

No visible effect on rate of transcription

In an experiment you add increasing amount of sigma factor to a mixture of DNA fragment and core polymerase in vitro. You also add [14C]ATP and [γ-32P]ATP in the mixture and check the rate of incorporation. Results show that the incorporation of both of this labels increase on adding more sigma factor. What will you conclude from your observation?

Sigma increases initiation of transcription rate

Sigma confers specificity and increases transcription rate

Sigma increases initiation and elongation rate

Sigma increases initiation of transcription rate

In an experiment you mutate the C terminal domain of alpha subunit of the RNA polymerase. What will you expect to see?

Transcription is at random sites

Which of these is the 1st event to take place during transcription initiation?

Formation of absorptive transcript

Formation of a closed initiation complex

Formation of open initiation complex

Formation of absorptive transcript

WHICH_OF_THESE_IS_THE_1ST_EVENT_TO_TAKE_PLACE_DURING_TRANSCRIPTION_INITIATION??$

Formation of absorptive transcript

Formation of a closed initiation complex

Formation of open initiation complex

Formation of absorptive transcript

In an experiment you mutate the C terminal domain of alpha subunit of the RNA polymerase. What will you expect to see?$

Transcription is at random sites

The_catalytic_unit_of_RNA_polymerases_when_placed_properly_during_initiation_is_just_over__________$

‚Äö√Ñ√∂‚àö√ë‚àö¬® 10 site

In an experiment, you wanted to see which mutation will have worse effect on transcription. In the 1st set you deleted 94 amino acids from the C-terminus and in the 2nd set you replace the 265th C-terminal arginine to cystine. Which in your opinion will have the worst effect?

No visible effect on rate of transcription

Both will have similar negative effect

No visible effect on rate of transcription

In an experiment you add increasing amount of sigma factor to a mixture of DNA fragment and core polymerase in vitro. You also add [14C]ATP and [‚âà√≠‚Äö√¢‚Ä¢-32P]ATP in the mixture and check the rate of incorporation. Results show that the incorporation of both of this labels increase on adding more sigma factor. What will you conclude from your observation?$

Sigma increases initiation of transcription rate

Sigma confers specificity and increases transcription rate

Sigma increases initiation and elongation rate

Sigma increases initiation of transcription rate

You want to find out which of the consequent gene X or Y is being transcribed. For this you do a hybridization competition analysis and plot the radioactivity curve with increasing concentration of the competitor unlabelled mRNA. If the transcribed gene was X, which observation fits your result?

The radioactivity of x and y decreases

The radioactivity of y decreases while that of x remains the same

The radioactivity of x decreases while that of y remains the same

The radioactivity of x and y decreases

The radioactivity of y decreases while x increases

You wanted to isolate active RNA polymerase (with a part of the transcript associated with it ) from cellular mix. For this, you perform an immunoprecipitation with anti sigma antibody. Then you check the precipitate. What will you observe?

RNA polymerase without transcript

DNA with associated RNA polymerase and a transcript

In an experiment you mutate the consensus sequence at the -10 box. You observe the rate of transcription reduces. Now you make complementary mutation to the sigma factor. What will you observe?

Increase in rate of random transcription

Further decrease in transcription rate

Increase in rate of random transcription

What is the direction in which the transcript produced by RNA polymerase grows?

3‚Äö√Ñ√∂‚àö√ë‚àö¬•->5‚Äö√Ñ√∂‚àö√ë‚àö¬• direction on 3‚Äö√Ñ√∂‚àö√ë‚àö¬•->5‚Äö√Ñ√∂‚àö√ë‚àö¬• strand

5‚Äö√Ñ√∂‚àö√ë‚àö¬•->3‚Äö√Ñ√∂‚àö√ë‚àö¬• direction on 5‚Äö√Ñ√∂‚àö√ë‚àö¬•->3‚Äö√Ñ√∂‚àö√ë‚àö¬• strand

3‚Äö√Ñ√∂‚àö√ë‚àö¬•->5‚Äö√Ñ√∂‚àö√ë‚àö¬• direction on 5‚Äö√Ñ√∂‚àö√ë‚àö¬•->3‚Äö√Ñ√∂‚àö√ë‚àö¬• strand

5‚Äö√Ñ√∂‚àö√ë‚àö¬•->3‚Äö√Ñ√∂‚àö√ë‚àö¬• direction on 3‚Äö√Ñ√∂‚àö√ë‚àö¬•->5‚Äö√Ñ√∂‚àö√ë‚àö¬• strand

The catalytic activity resides in which subunit of RNA polymerase?

‚âà√≠‚Äö√¢¬ß (151 kDa)

‚âà√≠‚Äö√¢¬ß‚Äö√Ñ√∂‚àö√ë‚Äö√¢¬ß (156 kDa)

‚âà√≠‚Äö√¢¬ß (151 kDa)

‚âà√¨‚àö√¢70 (70 kDa)

22 + Mcqs in Transcription Prokaryotes Initiation in Cytogenetics Page 1 McqOptions

1.	In an experiment, you wanted to see which mutation will have worse effect on transcription. In the 1st set you deleted 94 amino acids from the C-terminus and in the 2nd set you replace the 265th C-terminal arginine to cystine. Which in your opinion will have the worst effect?
A.	94 amino acid mutant
B.	265th amino acid mutant
C.	Both will have similar negative effect
D.	No visible effect on rate of transcription
Answer» D. No visible effect on rate of transcription

3.	Mutations as far as 100 base pairs upstream to +1 site will also affect the transcription rate.
A.	True
B.	False
Answer» B. False

4.	In an experiment you mutate the C terminal domain of alpha subunit of the RNA polymerase. What will you expect to see?
A.	Transcription is absent
B.	Transcription is at random sites
C.	Transcription is less
D.	Transcription is more
Answer» D. Transcription is more

5.	The catalytic unit of RNA polymerases when placed properly during initiation is just over _________
A.	-1 site
B.	0 site
C.	+1 site
D.	– 10 site
Answer» D. – 10 site

7.	The catalytic activity resides in which subunit of RNA polymerase?a) β′ (156 kDa)b) β (151 kDa)c) α (37 kDa)d) σ70 (70 kD
A.	β′ (156 kDa)
B.	β (151 kDa)
C.	α (37 kDa)
D.	σ70 (70 kDa)
Answer» B. β (151 kDa)

8.	WHICH_OF_THESE_IS_THE_1^ST_EVENT_TO_TAKE_PLACE_DURING_TRANSCRIPTION_INITIATION??$
A.	Formation of a closed initiation complex
B.	Formation of open initiation complex
C.	Formation of absorptive transcript
D.	Promoter clearance
Answer» C. Formation of absorptive transcript

11.	Which cation is placed in the catalytic subunit of RNA polymerase?
A.	Zn<sup>2+</sup>
B.	Mn<sup>2+</sup>
C.	Mg<sup>2+</sup>
D.	Fe<sup>3+</sup>
Answer» D. Fe<sup>3+</sup>

12.	In an experiment, you wanted to see which mutation will have worse effect on transcription. In the 1^st set you deleted 94 amino acids from the C-terminus and in the 2^nd set you replace the 265th C-terminal arginine to cystine. Which in your opinion will have the worst effect?
A.	94 amino acid mutant
B.	265<sup>th</sup> amino acid mutant
C.	Both will have similar negative effect
D.	No visible effect on rate of transcription
Answer» D. No visible effect on rate of transcription

14.	Mutations_as_far_as_100_base_pairs_upstream_to_+1_site_will_also_affect_the_transcription_rate.
A.	True
B.	False
Answer» B. False

16.	Which part of sigma factor associates with -10box?
A.	1.2
B.	2.4
C.	4.4
D.	4.2
Answer» C. 4.4

17.	You wanted to isolate active RNA polymerase (with a part of the transcript associated with it ) from cellular mix. For this, you perform an immunoprecipitation with anti sigma antibody. Then you check the precipitate. What will you observe?
A.	RNA polymerase with transcript
B.	RNA polymerase without transcript
C.	Only antibody
D.	DNA with associated RNA polymerase and a transcript
Answer» C. Only antibody

22.	Which protein mentioned below can reverse central dogma?
A.	Ribosome
B.	Restriction Endonuclease
C.	Reverse Transcriptase
D.	RNA Polymerase
Answer» D. RNA Polymerase

18.	In an experiment you mutate the consensus sequence at the -10 box. You observe the rate of transcription reduces. Now you make complementary mutation to the sigma factor. What will you observe?
A.	Further decrease in transcription rate
B.	Increase in transcription rate
C.	Original transcription rate
D.	Increase in rate of random transcription
Answer» D. Increase in rate of random transcription

19.	In an experiment you use RNA polymerase without its sigma factor for transcription. What will be the result that you observe?
A.	More transcription
B.	Less transcription
C.	More specific transcription
D.	More random transcription
Answer» E.

Explore topic-wise MCQs in Cytogenetics.

In an experiment, you wanted to see which mutation will have worse effect on transcription. In the 1st set you deleted 94 amino acids from the C-terminus and in the 2nd set you replace the 265th C-terminal arginine to cystine. Which in your opinion will have the worst effect?

Mutations as far as 100 base pairs upstream to +1 site will also affect the transcription rate.

In an experiment you mutate the C terminal domain of alpha subunit of the RNA polymerase. What will you expect to see?

The catalytic unit of RNA polymerases when placed properly during initiation is just over _________

Which of these is the 1st event to take place during transcription initiation?

The catalytic activity resides in which subunit of RNA polymerase?a) β′ (156 kDa)b) β (151 kDa)c) α (37 kDa)d) σ70 (70 kD

WHICH_OF_THESE_IS_THE_1ST_EVENT_TO_TAKE_PLACE_DURING_TRANSCRIPTION_INITIATION??$

In an experiment you mutate the C terminal domain of alpha subunit of the RNA polymerase. What will you expect to see?$

The_catalytic_unit_of_RNA_polymerases_when_placed_properly_during_initiation_is_just_over__________$

Which cation is placed in the catalytic subunit of RNA polymerase?

In an experiment, you wanted to see which mutation will have worse effect on transcription. In the 1st set you deleted 94 amino acids from the C-terminus and in the 2nd set you replace the 265th C-terminal arginine to cystine. Which in your opinion will have the worst effect?

Mutations_as_far_as_100_base_pairs_upstream_to_+1_site_will_also_affect_the_transcription_rate.

You want to find out which of the consequent gene X or Y is being transcribed. For this you do a hybridization competition analysis and plot the radioactivity curve with increasing concentration of the competitor unlabelled mRNA. If the transcribed gene was X, which observation fits your result?

Which part of sigma factor associates with -10box?

You wanted to isolate active RNA polymerase (with a part of the transcript associated with it ) from cellular mix. For this, you perform an immunoprecipitation with anti sigma antibody. Then you check the precipitate. What will you observe?

In an experiment you mutate the consensus sequence at the -10 box. You observe the rate of transcription reduces. Now you make complementary mutation to the sigma factor. What will you observe?

In an experiment you use RNA polymerase without its sigma factor for transcription. What will be the result that you observe?

What is the direction in which the transcript produced by RNA polymerase grows?

The catalytic activity resides in which subunit of RNA polymerase?

Which protein mentioned below can reverse central dogma?

WHICH_OF_THESE_IS_THE_1^ST_EVENT_TO_TAKE_PLACE_DURING_TRANSCRIPTION_INITIATION??$

In an experiment, you wanted to see which mutation will have worse effect on transcription. In the 1^st set you deleted 94 amino acids from the C-terminus and in the 2^nd set you replace the 265th C-terminal arginine to cystine. Which in your opinion will have the worst effect?