MCQOPTIONS
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MCQOPTIONS
This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Vector Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The derivatives of M13 vector are exact counterparts of _________ |
| A. | tPbr322 |
| B. | tPUC |
| C. | tLambda |
| D. | tAdenovirus |
| Answer» C. tLambda | |
| 2. |
The M13 mp2 contained ________ |
| A. | tCloning site |
| B. | tNuclease |
| C. | tCodon |
| D. | tEcoR1 site |
| Answer» E. | |
| 3. |
Which process was used to create an EcoR1 site in the lac region of coliphage vectors? |
| A. | tHybridization |
| B. | tMutagenesis |
| C. | tReplication |
| D. | tRestriction |
| Answer» C. tReplication | |
| 4. |
The lac region does not contain any restriction site for which of the following? |
| A. | tAvaII |
| B. | tPvuII |
| C. | tEcoR1 |
| D. | tBglII |
| Answer» D. tBglII | |
| 5. |
Insertion of fragments into lac region destroys __________ |
| A. | tBlue color |
| B. | tWhite color |
| C. | tTransparency |
| D. | tOpaqueness |
| Answer» B. tWhite color | |
| 6. |
The first use of M13 as a cloning vector made use of _______ sites. |
| A. | t4 |
| B. | t8 |
| C. | t10 |
| D. | t12 |
| Answer» D. t12 | |
| 7. |
The M13 intergenic sequence contains ________ |
| A. | tOrigins |
| B. | tTermination sequence |
| C. | tReplication site |
| D. | tNucleosome |
| Answer» B. tTermination sequence | |
| 8. |
Unlike lambda, filamentous phages do not have ____________ |
| A. | tCodons |
| B. | tNucleases |
| C. | tCloning sites |
| D. | tTranscription factors |
| Answer» D. tTranscription factors | |
| 9. |
Filamentous phages do not possess the advantages of plasmids. |
| A. | tTrue |
| B. | tFalse |
| Answer» C. | |
| 10. |
Agarose gel electrophoresis is used to determine the _________ of the insert. |
| A. | tActivity |
| B. | tSize |
| C. | tOrientation |
| D. | tReplication |
| Answer» D. tReplication | |
| 11. |
There are no packaging constraints in single-stranded vectors. |
| A. | tTrue |
| B. | tFalse |
| Answer» B. tFalse | |
| 12. |
RF and single-stranded DNA __________ the competent E.coli cells. |
| A. | tInfect |
| B. | tTransfect |
| C. | tKill |
| D. | tTranslate |
| Answer» C. tKill | |
| 13. |
RF acts just like a ___________ |
| A. | tPhage |
| B. | tHost |
| C. | tPlasmid |
| D. | tHybrid |
| Answer» D. tHybrid | |
| 14. |
Single stranded form combines cloning, amplification and __________________ of and originally double-stranded DNA fragment. |
| A. | tStrand separation |
| B. | tReplication |
| C. | tTranscription |
| D. | tTranslation |
| Answer» B. tReplication | |
| 15. |
Single-stranded DNA was used in __________ |
| A. | tDideoxy method |
| B. | tReplication |
| C. | tTranslational |
| D. | tHybridization |
| Answer» B. tReplication | |