MCQOPTIONS
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MCQOPTIONS
This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Vector Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
FIGE is the modification of which technique? |
| A. | OFAGE |
| B. | Agarose gel electrophoresis |
| C. | Electrophoresis |
| D. | Acrylamide gel electrophoresis |
| Answer» B. Agarose gel electrophoresis | |
| 2. |
Which of the following techniques cannot be used to separate the genome of lower eukaryote yeast? |
| A. | CHEF |
| B. | OGAGE |
| C. | FIGE |
| D. | Gel electrophoresis |
| Answer» E. | |
| 3. |
What is the full form of OFAGE? |
| A. | Orthogonal field alternation gel electrophoresis |
| B. | Orthogonal field attraction gel electrophoresis |
| C. | Oligonucleotide field alternation gel electrophoresis |
| D. | Oligonucleotide field attraction gel electrophoresis |
| Answer» B. Orthogonal field attraction gel electrophoresis | |
| 4. |
What does a complex pattern of bands in a gel electrophoresis signify? |
| A. | Partial digestion |
| B. | Double digestion |
| C. | Complete digestion |
| D. | Contaminated sample |
| Answer» B. Double digestion | |
| 5. |
What will happen if the incubation period, post restriction digestion is shortened? |
| A. | Indigestion |
| B. | Partial digestion |
| C. | Double digestion |
| D. | Complete digestion |
| Answer» C. Double digestion | |
| 6. |
What are double digestions? |
| A. | Cutting with an enzyme twice on the same site |
| B. | Cutting with two different enzymes on the same site |
| C. | Cutting with an enzyme twice on different sites |
| D. | Cutting with two different enzymes on the different sites |
| Answer» E. | |
| 7. |
How many basepairs long DNA molecules can be distinguished by the polyacrylamide (40%) gel electrophoresis? |
| A. | 1-100 kbp |
| B. | 1-300 bp |
| C. | 100-200 kbp |
| D. | 300-600 bp |
| Answer» C. 100-200 kbp | |
| 8. |
The rate of migration of a DNA molecule in an electrophoresis depends on which two factors? |
| A. | Temperature, length |
| B. | Length, source |
| C. | Shape, charge-to-mass ratio |
| D. | Shape, viscosity |
| Answer» D. Shape, viscosity | |
| 9. |
How can the sizes of individual cleavage products be determined? |
| A. | Temperature check |
| B. | Molecular weight check |
| C. | Chromatography |
| D. | Gel electrophoresis |
| Answer» E. | |
| 10. |
How can it be determined that a DNA molecule is cleaved by the action of endonuclease? |
| A. | Checking the viscosity of the solution |
| B. | Temperature check |
| C. | Color indication |
| D. | pH check |
| Answer» B. Temperature check | |
| 11. |
Restriction digests with which enzyme needs to be kept at a higher temperature than the usual 37 degree temperature? |
| A. | TaqI |
| B. | EcoRI |
| C. | BamHI |
| D. | HindIII |
| Answer» B. EcoRI | |
| 12. |
What is dithiothreitol? |
| A. | Reducing agent |
| B. | Restriction endonuclease |
| C. | Ligase |
| D. | Exonuclease |
| Answer» B. Restriction endonuclease | |
| 13. |
Which of the following does not produce cohesive ends upon cleavage on their recognition sequences? |
| A. | EcoRI |
| B. | PvuII |
| C. | BamHI |
| D. | HindIII |
| Answer» C. BamHI | |
| 14. |
Which of the following is an example of flush end cutter? |
| A. | PvuI |
| B. | PvuII |
| C. | EcoR1 |
| D. | SfiI |
| Answer» C. EcoR1 | |
| 15. |
EcoR1 has which of the following hexanucleotide recognition sequence? |
| A. | GAATTC |
| B. | GGATCC |
| C. | AGATCT |
| D. | GCAGCA |
| Answer» B. GGATCC | |