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This section includes 5 Mcqs, each offering curated multiple-choice questions to sharpen your Cytogenetics knowledge and support exam preparation. Choose a topic below to get started.
1. |
Methylated DNA doesn t form kink as________________ |
A. | As they don t fit in the active site |
B. | The enzyme recognized methylated A as different base than non-methylated A |
C. | They are less flexible so no kink is formed |
D. | They don t interact with the side chains in an active site |
Answer» E. | |
2. |
The Mg2+ ion in restriction endonuclease is not bound to ________ |
A. | Water molecule |
B. | Carboxylates of the enzymes aspertate group |
C. | Oxygen |
D. | Histidine |
Answer» E. | |
3. |
Restriction enzymes cleave the bond by which type of reaction? |
A. | S<sub>N</sub>1 |
B. | S<sub>N</sub>2 |
C. | S<sub>E</sub>1 |
D. | S<sub>E</sub>2 |
Answer» C. S<sub>E</sub>1 | |
4. |
Which of these restriction enzymes generate 3 overhang? |
A. | Sph1 |
B. | EcoR1 |
C. | Sma1 |
D. | Xma1 |
Answer» B. EcoR1 | |
5. |
What does this sequence GC/GGCCGC recognized and cleaves by Not1 Doesn t agree to? |
A. | The cleavage is after the 2<sup>nd</sup> C from 5 end |
B. | The cleavage generates a 5 overhang |
C. | This sequence has a probability of occurrence in every 1024 bases |
D. | This cleavage is asymmetric |
Answer» D. This cleavage is asymmetric | |