MCQOPTIONS
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MCQOPTIONS
This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Vector Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
WHAT_IS_THE_PROBLEM_ASSOCIATED_WITH_ADAPTORS??$ |
| A. | Shorter and hence improper ligation |
| B. | Less efficient than linkers |
| C. | May self-ligate and hence require restriction |
| D. | Cannot be made synthetically |
| Answer» D. Cannot be made synthetically | |
| 2. |
What is the function of a polynucleotide kinase?$ |
| A. | Removal of the phosphate group from 5’ end |
| B. | Removal of the phosphate group from 3’ end |
| C. | Addition of phosphate group on 5’ end |
| D. | Addition of phosphate group on 3’ end |
| Answer» D. Addition of phosphate group on 3‚Äö√Ñ√∂‚àö√ë‚àö¬• end | |
| 3. |
Ligation_takes_place_between___________$ |
| A. | Adaptor and linker |
| B. | Linker and vector |
| C. | 5’-P terminus and 3’-OH terminus |
| D. | Adaptor and vector |
| Answer» D. Adaptor and vector | |
| 4. |
Which of the following is not a function of DNA topoisomerase? |
| A. | Removing turns of the double helix DNA |
| B. | Adding turns to the double helix DNA |
| C. | Have nuclease and ligase activity |
| D. | Have polymerase activity |
| Answer» E. | |
| 5. |
A recombinant DNA molecule held together by base-pairing although not completely ligated can be introduced into the host cell. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 6. |
Which of the following statements is true in case of homopolymer tailing for producing sticky ends? |
| A. | Poly(dC) tails to vector, poly(dG) to DNA |
| B. | Poly(dG) tails to vector, poly(dC) to DNA |
| C. | Poly(dC) tails to vector and DNA |
| D. | Poly(dC) tails to vector and DNA |
| Answer» B. Poly(dG) tails to vector, poly(dC) to DNA | |
| 7. |
Which_enzyme_is_used_in_homopolymer_tailing_for_producing_sticky_ends? |
| A. | Terminal deoxynucleotidyl transferase |
| B. | Alkaline phosphatase |
| C. | Polynucleotide kinase |
| D. | DNA polymerase |
| Answer» B. Alkaline phosphatase | |
| 8. |
What is the problem associated with the use of linkers for putting sticky ends to a blunt ended molecule? |
| A. | High ambient temperature requirements |
| B. | Possible cleavage of the DNA molecule itself |
| C. | High cost of synthetic linkers |
| D. | Low compatibility of linkers |
| Answer» C. High cost of synthetic linkers | |
| 9. |
What are Linkers? |
| A. | Short synthetic double stranded DNA sequence |
| B. | Short oligonucleotide sequence of host |
| C. | Short oligonucleotide sequence of vector |
| D. | Short synthetic single stranded DNA sequence |
| Answer» B. Short oligonucleotide sequence of host | |
| 10. |
Which of the following is not a method for putting sticky ends to a blunt ended DNA fragment to be cloned? |
| A. | Homopolymer tailing |
| B. | Adaptors |
| C. | Restriction digestion |
| D. | Linkers |
| Answer» D. Linkers | |
| 11. |
Which of the following will have more efficient ligation? |
| A. | Sticky ends |
| B. | Blunt ends |
| C. | Blunt ends and high concentration of DNA |
| D. | Blunt ends and low concentration of DNA |
| Answer» B. Blunt ends | |
| 12. |
The chemical reaction involved in ligation of two molecules is same as repairing discontinuities. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 13. |
Within the cell, ligase enzyme has the function of repairing any discontinuities that may arise in the double stranded DNA molecule. What are these discontinuities? |
| A. | Absence of nucleotides in 1 strand |
| B. | Missing phosphodiester bond in 1 strand |
| C. | Missing phosphodiester bond in 2 both strands |
| D. | Absence of nucleotides in both strands |
| Answer» C. Missing phosphodiester bond in 2 both strands | |
| 14. |
Which DNA ligase enzyme is used in genetic engineering? |
| A. | Bacterial ligase |
| B. | T4 ligase |
| C. | Yeast ligase |
| D. | Pseudomonas ligase |
| Answer» C. Yeast ligase | |
| 15. |
Which is the final step in the construction of a recombinant molecule? |
| A. | Plasmid isolation |
| B. | Gene amplification |
| C. | Restriction digestion |
| D. | Ligation |
| Answer» E. | |