MCQOPTIONS
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MCQOPTIONS
This section includes 4 Mcqs, each offering curated multiple-choice questions to sharpen your Vector Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which enzyme is used in homopolymer tailing for producing sticky ends? |
| A. | Terminal deoxynucleotidyl transferase |
| B. | Alkaline phosphatase |
| C. | Polynucleotide kinase |
| D. | DNA polymerase |
| Answer» B. Alkaline phosphatase | |
| 2. |
What is the function of a polynucleotide kinase? |
| A. | Removal of the phosphate group from 5 end |
| B. | Removal of the phosphate group from 3 end |
| C. | Addition of phosphate group on 5 end |
| D. | Addition of phosphate group on 3 end |
| Answer» D. Addition of phosphate group on 3 end | |
| 3. |
Ligation takes place between __________ |
| A. | Adaptor and linker |
| B. | Linker and vector |
| C. | 5 -P terminus and 3 -OH terminus |
| D. | Adaptor and vector |
| Answer» D. Adaptor and vector | |
| 4. |
What is the problem associated with adaptors? |
| A. | Shorter and hence improper ligation |
| B. | Less efficient than linkers |
| C. | May self-ligate and hence require restriction |
| D. | Cannot be made synthetically |
| Answer» D. Cannot be made synthetically | |