MCQOPTIONS
Saved Bookmarks
MCQOPTIONS
This section includes 56 Mcqs, each offering curated multiple-choice questions to sharpen your Genetics knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Peptidyl transferase activity resides in |
| A. | 16S rRNA |
| B. | 23 rRNA |
| C. | 5S rRNA |
| D. | 28 rRNA |
| Answer» C. 5S rRNA | |
| 2. |
During transcription |
| A. | DNA Gyrase introduces negative supercoils and DNA Topoisomerase I removes negative supercoils |
| B. | DNA Topoisomerase I introduces negative supercoils and DNA Gyrase removes negative supercoils |
| C. | both DNA Gyrase and DNA |
| D. | Topoisomearse I introduce negative supercoils |
| E. | both DNA Gyrase and DNA Topoisomearse I remove negative supercoils |
| Answer» B. DNA Topoisomerase I introduces negative supercoils and DNA Gyrase removes negative supercoils | |
| 3. |
How many 3-tuples are possible for the following amino acid sequence ? madcmwdisease |
| A. | 4 |
| B. | 5 |
| C. | 11 |
| D. | 12 |
| Answer» D. 12 | |
| 4. |
The double stranded DNA molecule of a virus was found by electron microscopy to have a length of 34 M. |
| A. | How many nucleotide pairs are there in one molecule ? |
| B. | How many complete turns of the two polynucleotide chains are present in such a double helix ? |
| C. | How many electron microscopy are there in one molecule ? |
| D. | How many DNA molecule are there in one molecule ? |
| E. | None Of These |
| Answer» F. | |
| 5. |
The term protein splicing refers to |
| A. | Removal of intervening sequences between the genes |
| B. | Splicing out of introns from RNA |
| C. | Removal of intervening protein sequences from the translated protein |
| D. | Joining (splicing) of two different gene products to generate a novel protein |
| Answer» D. Joining (splicing) of two different gene products to generate a novel protein | |
| 6. |
In eukaryotes the ribosomal RNA genes are transcribed by |
| A. | Reverse transcriptase |
| B. | RNA dependent RNA polymerase |
| C. | RNA polymerase I |
| D. | RNA polymerase III |
| Answer» D. RNA polymerase III | |
| 7. |
Rho-dependent and rho-independent transcription termination mechanisms operate in prokaryotes. Rho independent termination mechanism involves |
| A. | Binding of the rho protein upstream of the termination element |
| B. | No protein factors and only RNA secondary structure and run of U s |
| C. | Presence of UGA or UAA stop codons |
| D. | Binding of accessory factors at termination signal |
| Answer» C. Presence of UGA or UAA stop codons | |
| 8. |
The G + C content of bacteriophage 13 double standed DNA is 68%. What would you expect the G + C content of its mRNA ? |
| A. | About 68% |
| B. | About 34% |
| C. | About 32% |
| D. | About 86% |
| Answer» C. About 32% | |
| 9. |
Reverse genetics means |
| A. | Finding the function of a ORF |
| B. | Finding the gene responsible for a trait |
| C. | RNA dependent DNA synthesis |
| D. | Converting somatic cell to a germ cell |
| Answer» B. Finding the gene responsible for a trait | |
| 10. |
A heterologous protein for its expression in the milk of a transgenic animal should be under the control of the promoter of the gene coding for |
| A. | -Globin |
| B. | -Lactoglobulin |
| C. | Preproinsulin |
| D. | lac Z |
| Answer» C. Preproinsulin | |
| 11. |
The major groove of DNA is lined by |
| A. | N3 of purine and N1 of pyrimidine |
| B. | N7 of purine and O2 of pyrimidine |
| C. | N7 of purine and C4 of Pyrimidine |
| D. | None of these |
| Answer» D. None of these | |
| 12. |
The fundamental feature of the genetic code which allows the expression of a protein in any host is its |
| A. | Triplet nature |
| B. | Universality |
| C. | Degeneracy |
| D. | Redundancy |
| Answer» D. Redundancy | |
| 13. |
Efficient expression of a heterologous protein product is influenced by |
| A. | Transcriptional efficiency |
| B. | Copy number of the plasmid |
| C. | Codon bias |
| D. | All of these |
| Answer» E. | |
| 14. |
Which of the following processes require energy? |
| A. | ligation |
| B. | transformation |
| C. | restriction digestion |
| D. | hybridization |
| Answer» B. transformation | |
| 15. |
RNA is very much susceptible to hydrolysis in alkali because |
| A. | It contains uracil residues in its structure |
| B. | Its 2 -OH group participate in intramolecular cleavage of phosphodiester backbone |
| C. | Cleavage occurs in the glycosylic bonds of purine bases |
| D. | Cleavage occurs in the glycosylic bonds of pyrimidine bases |
| Answer» C. Cleavage occurs in the glycosylic bonds of purine bases | |
| 16. |
The full-length coding sequence of an eukaryotic gene was expressed in bacteria and the protein was purified. However, in the functionalassay, no activity was detected for the purified protein. The reason could be |
| A. | the host bacteria produced an enzyme that inhibited the activity of the expressed eukaryotic protein |
| B. | the purified protein was contaminated with bacteria |
| C. | the host bacteria did not produce the essential co-factors |
| D. | no post-translational modification on the protein expressed in bacteria |
| Answer» B. the purified protein was contaminated with bacteria | |
| 17. |
Which of the following genes are involved in Immortalization of primary hepatocytes? |
| A. | Telomerase and Cyclin D |
| B. | NF B and Thymidine kinase |
| C. | Cyclin D and myc |
| D. | Telomerase and Ras |
| Answer» E. | |
| 18. |
Expression of which of the following reporter genes does not require addition of specific substrate for detection ? |
| A. | Luciferase |
| B. | -Glucuronidase |
| C. | -Glucosidase |
| D. | Green fluorescent protein |
| Answer» B. -Glucuronidase | |
| 19. |
The enzymes that can be used in 5 end labeling of DNA are |
| A. | P, S |
| B. | R, Q |
| C. | P, R |
| D. | R, S |
| Answer» B. R, Q | |
| 20. |
Mobile genetic elements present in human genome are |
| A. | Q, R |
| B. | P, Q |
| C. | P, R |
| D. | Q, S |
| Answer» C. P, R | |
| 21. |
Presence of CX |
| A. | a protein kinase |
| B. | GTP binding protein |
| C. | zinc finger protein |
| D. | lipase |
| Answer» D. lipase | |
| 22. |
Si RNA(s) interfere at |
| A. | transcriptional level |
| B. | post-transcriptional level |
| C. | DNA replication level |
| D. | translational level |
| Answer» C. DNA replication level | |
| 23. |
Zinc fingers are characteristics of |
| A. | blood clotting proteins |
| B. | RNA chaperones |
| C. | DNA binding proteins |
| D. | lysosomal hydrolases |
| Answer» D. lysosomal hydrolases | |
| 24. |
Determine the correctness or otherwise of the following Assertion (a) and Reason (r): |
| A. | Both (a) and (r) are true and (r) is the correct reason for (a) |
| B. | Both (a) and (r) are true but (r) is not the correct reason for (a) |
| C. | (a) is true but (r) is false |
| D. | (a) is false but (r) is true |
| Answer» C. (a) is true but (r) is false | |
| 25. |
Expression of antisense RNA of ACC synthase in transgenic tomato plants inhibited the synthesis of ethylene resulting in |
| A. | Change in colour from green to red |
| B. | Change in aroma |
| C. | Change in colour from red to green |
| D. | None of these |
| Answer» E. | |
| 26. |
What would happen to the mutant cells by ionizing radiation? |
| A. | Apoptosis |
| B. | Necrosis |
| C. | Cell growth arrest |
| D. | Cell proliferation |
| Answer» D. Cell proliferation | |
| 27. |
Which of the following are commonly used as reporter genes? |
| A. | Q, S |
| B. | R,S |
| C. | P, R |
| D. | P, Q |
| Answer» B. R,S | |
| 28. |
Match the following antibiotics in Group I with their mode of action in Group II |
| A. | P-1, Q-3, R-2, S-4 |
| B. | P-3, Q-1, R-2, S-4 |
| C. | P-3, Q-1, R-4, S-2 |
| D. | P-4, Q-2, R-3, S-1 |
| Answer» D. P-4, Q-2, R-3, S-1 | |
| 29. |
Which set of the following events occurs during the elongation step of translation? |
| A. | P, Q and R |
| B. | P, Q and T |
| C. | Q, R and T |
| D. | R, S and T |
| Answer» D. R, S and T | |
| 30. |
Determine the correctness of the following statements |
| A. | only I is true |
| B. | only II is true |
| C. | both I and II are true |
| D. | both I and II are false |
| Answer» C. both I and II are true | |
| 31. |
Topological winding number of a 2.0 kb covalently closed circular DNA was found to be 191 with a writhing number of 4. Hence, its LINKING NUMBER and the NUMBER OF BASE PAIR PER TURN when the molecule is laid flat on the surface is _______ and__________, respectively. |
| A. | 187, 10.69 |
| B. | 195, 10.25 |
| C. | 200, 10.00 |
| D. | 187, 10.50 |
| Answer» B. 195, 10.25 | |
| 32. |
The 5 ends of the mature forms of the prokaryotic mRNAs and tRNAs are |
| A. | a triphosphate group in mRNAs and a monophosphate group in tRNAs |
| B. | triphosphate groups in both mRNAs and tRNAs |
| C. | monophosphate groups in both mRNAs and tRNAs |
| D. | a monophosphate group in mRNAs and a triphosphate group in tRNAs |
| Answer» B. triphosphate groups in both mRNAs and tRNAs | |
| 33. |
An in vitor translation system can synthesize peptides in all three reading frames of the RNA template. When 5 -UCUCUCUC---(UC) |
| A. | UCU |
| B. | CUC |
| C. | CCU |
| D. | UCC |
| Answer» D. UCC | |
| 34. |
Match the drugs in Group I with their mechanism of action in Group II. |
| A. | P-1, Q-6, R-3, S-4 |
| B. | P-2, Q-6, R-3, S-5 |
| C. | P-1, Q-3, R-6, S-5 |
| D. | P-2, Q-3, R-6, S-4 |
| Answer» C. P-1, Q-3, R-6, S-5 | |
| 35. |
Under alkaline conditions, DNA is more stable than RNA because |
| A. | RNA forms secondary structures |
| B. | RNA is a single stranded molecule |
| C. | RNA has uracil in place of thymidine |
| D. | RNA is susceptible to hydrolysis |
| Answer» E. | |
| 36. |
The active site in the alpha/beta barrel structures is usually located |
| A. | inside the barrel |
| B. | at the amino side of the strands |
| C. | at the carboxy side of the strands |
| D. | at any arbitrary site |
| Answer» D. at any arbitrary site | |
| 37. |
For their efficient translation, eubacterial mRNAs possess a Shine-Dalgarno sequence for its recognition by an anti-Shine-Dalgarno sequence (ASD) in the ribosomes. The correct statement is |
| A. | ASD is present in 5S rRNA |
| B. | ASD is present in 23S rRNA |
| C. | ASD is present in 16S rRNA |
| D. | ASD is formed by the interaction of the 16S rRNA with the 23S rRNA upon docking of the 50S subunit on the 30S subunit of the ribosomes |
| Answer» D. ASD is formed by the interaction of the 16S rRNA with the 23S rRNA upon docking of the 50S subunit on the 30S subunit of the ribosomes | |
| 38. |
The percentage SIMILARITIES and IDENTITIES, respectively, between the two peptide sequences given below will be ______% and _____%. |
| A. | 80, 80 |
| B. | 80, 60 |
| C. | 60, 60 |
| D. | 90, 60 |
| Answer» C. 60, 60 | |
| 39. |
The total number of fragments generated by the complete and sequential cleavage of the polypeptide given below by Trypsin followed by CNBr is _______ |
| A. | 1 |
| B. | 3 |
| C. | 5 |
| D. | 7 |
| Answer» D. 7 | |
| 40. |
Diphtheria toxin, tetracycline and streptomycin inhibit |
| A. | DNA repair |
| B. | DNA replication |
| C. | transcription |
| D. | translation |
| Answer» E. | |
| 41. |
Molecular chaperones are class of proteins that facilitate |
| A. | the proper folding of newly synthesized proteins |
| B. | unfolding of newly synthesized proteins |
| C. | degradation of newly synthesized proteins |
| D. | targeting of newly synthesized proteins |
| Answer» B. unfolding of newly synthesized proteins | |
| 42. |
In zinc finger proteins, the amino acid residues that coordinate zinc are |
| A. | Cys and His |
| B. | Asp and Glu |
| C. | Arg and Lys |
| D. | Asp and Arg |
| Answer» B. Asp and Glu | |
| 43. |
Determine the correctness or otherwise of the following Asertion (a) and the Reason (r) |
| A. | both (a) and (r) are true and (r) is the correct reason for (a) |
| B. | both (a) and (r) are true and (r) is not the correct reason for (a) |
| C. | (a) is true but (r) is false |
| D. | (a) is false but (r) is true |
| Answer» E. | |
| 44. |
Nuclease-hypersensitive sites in the chromosomes are sites that appear to be |
| A. | H2 and H4 histone free |
| B. | H1 and H2 histone free |
| C. | H3 and H4 histone free |
| D. | Nucleosome free |
| Answer» E. | |
| 45. |
Which of the following characteristics with respect to bacterial DNA polymerase III are TRUE? |
| A. | P and Q only |
| B. | Q and R only |
| C. | Q and S only |
| D. | P and S only |
| Answer» C. Q and S only | |
| 46. |
The direction of shell coiling in the snail Limnaea peregra is a classic example of |
| A. | chromosomal inheritance |
| B. | extra-chromosomal inheritance |
| C. | chromosomal translocation |
| D. | homologous recombination |
| Answer» C. chromosomal translocation | |
| 47. |
A truncated polypeptide is synthesized due to a nonsense mutation. Where would you introduce another mutation to obtain a full-length polypeptide? |
| A. | Ribosomal protein gene |
| B. | Transfer RNA gene |
| C. | DNA repair gene |
| D. | Ribosomal RNA gene |
| Answer» C. DNA repair gene | |
| 48. |
A protein is phosphorylated at a serine residue. A phosphomimic mutant of the protein can be generated by substituting that serine with |
| A. | glycine |
| B. | alanine |
| C. | aspartate |
| D. | threonine |
| Answer» D. threonine | |
| 49. |
In mismatch correction repair, the parental DNA strand is distinguished from the daughter strand by _____________ |
| A. | acetylation |
| B. | phosphorylation |
| C. | methylation |
| D. | glycosylation |
| Answer» D. glycosylation | |
| 50. |
Match the entries in Group Iwith the entries in Group II. |
| A. | P-3, Q-4, R-2, S-1 |
| B. | P-4, Q-3, R-2, S-1 |
| C. | P-3, Q-2, R-1, S-4 |
| D. | P-2, Q-4, R-1, S-3 |
| Answer» B. P-4, Q-3, R-2, S-1 | |