

MCQOPTIONS
This section includes 74 Mcqs, each offering curated multiple-choice questions to sharpen your Transcription knowledge and support exam preparation. Choose a topic below to get started.
1. |
Which of the following is not a type of RNA? |
A. | mRNA |
B. | cRNA |
C. | rRNA |
D. | tRNA |
Answer» C. rRNA | |
2. |
What are snRNPs (small nuclear ribonucleic particles)? |
A. | RNA molecules that convey genetic information from DNA to the ribosome |
B. | RNA molecules that serve as the link between the nucleotide sequence of nucleic acids and the amino acid sequence of proteins |
C. | RNA component of the ribosome, essential for protein synthesis |
D. | RNA protein complexes that combine with pre-mRNA and other proteins to form a spliceosome |
Answer» E. | |
3. |
What are exons? |
A. | Site where repressor proteins bind |
B. | Genetic information coding for an amino acid sequence that will form a functional protein |
C. | Set of genes that are adjacent to one another in the genome and are coordinately controlled |
D. | Intervening non-coding segments of DNA |
Answer» C. Set of genes that are adjacent to one another in the genome and are coordinately controlled | |
4. |
Which of the following processes is not a type of RNA processing carried out by eukaryotes? |
A. | Polyadenylation of the 3' end |
B. | Nucleotide excision repair |
C. | Capping of the 5' end |
D. | Splicing and removal of introns |
Answer» C. Capping of the 5' end | |
5. |
During transcription of a eukaryotic cell, what determines the template strand? |
A. | The base sequence of the operator |
B. | The base sequence of the promoter |
C. | The location of the MET start codon |
D. | The base sequence of the enhancer |
Answer» C. The location of the MET start codon | |
6. |
Which of the following best describes distant regulatory sequences that influence gene expression? |
A. | Transcriptional start sites |
B. | Introns |
C. | Promoters |
D. | Enhancers |
Answer» E. | |
7. |
What event is indicative of transcription initiation? |
A. | Telomerase lengthens the telomeres of the DNA |
B. | The RNA strand begins to be synthesized |
C. | RNA polymerase is removed from the DNA |
D. | RNA polymerase binds to the promoter |
Answer» E. | |
8. |
When does alternative splicing occur? |
A. | Following mRNA polyadenylation |
B. | Following mRNA loading into ribosome |
C. | Following pre-mRNA transcription in the nucleus |
D. | Following mRNA translocation to the cytosol |
Answer» D. Following mRNA translocation to the cytosol | |
9. |
Which of the following are methods for termination of transcription in prokaryotes? I. Release factor binding II. Rho-mediated termination III. Hairpin loop (stem loop) formation |
A. | III only |
B. | I, II, and III |
C. | II and III |
D. | I only |
Answer» D. I only | |
10. |
What element(s) do all eukaryotic promoter regions share? |
A. | All of these |
B. | A basal promoter (TATA box) |
C. | None of these |
D. | Enhancers that accept binding agents |
Answer» B. A basal promoter (TATA box) | |
11. |
How many base pairs of DNA is transcribed by RNA polymerase in one go? |
A. | 5-6 |
B. | 3 |
C. | 4 |
D. | 7-8 |
Answer» E. | |
12. |
Which of the following is used to describe the time taken by RNA polymerase to leave the promoter? |
A. | Promoter clearance time |
B. | Promoter clearance time |
C. | Elongation factor |
D. | Mean time |
Answer» B. Promoter clearance time | |
13. |
Which of the following ensure stable binding of RNA polymerase at the promoter site? |
A. | DNA photolyase |
B. | Sigma factor |
C. | DNA glycosylase |
D. | RecA |
Answer» C. DNA glycosylase | |
14. |
What is the work of the sigma factor in transcription? |
A. | Helicase action |
B. | Transcription initiation |
C. | Transcription elongation |
D. | Transcription termination |
Answer» C. Transcription elongation | |
15. |
Name the sigma factor which is used for promoter recognition? |
A. | Sigma 32 |
B. | Sigma 70 |
C. | Sigma 60 |
D. | Sigma 40 |
Answer» C. Sigma 60 | |
16. |
Who discovered RNA polymerase? |
A. | Samuel B. Weiss |
B. | Nirenberg |
C. | Watson and Crick |
D. | Darwin |
Answer» B. Nirenberg | |
17. |
Which of the following is TRUE for the RNA polymerase activity? |
A. | DNA dependent DNA synthesis |
B. | Direct repair |
C. | DNA dependent RNA synthesis |
D. | RNA dependent RNA synthesis |
Answer» D. RNA dependent RNA synthesis | |
18. |
Name the site where upstream sequences located? |
A. | Prior to start point |
B. | After the startpoint |
C. | Right border of DNA |
D. | In the middle of DNA |
Answer» B. After the startpoint | |
19. |
Mark the statement which is INCORRECT about the transcription unit? |
A. | It is a transcribed segment of DNA |
B. | Eukaryotes have monocistronic transcription unit |
C. | Prokaryotes also have a monocistronic transcription unit |
D. | Immediate product of transcription is primary transcript |
Answer» D. Immediate product of transcription is primary transcript | |
20. |
Like replication, transcription also occurs bidirectionally. |
A. | True |
B. | False |
Answer» C. | |
21. |
Which polymerase is involved in transcribing ribosomal RNA (except 5S rRNA)? |
A. | DNA polymerase V |
B. | RNA polymerase III |
C. | RNA polymerase I |
D. | DNA polymerase IV |
Answer» D. DNA polymerase IV | |
22. |
Which of the following is not dependent on the C-terminal domain (CTD) of RNA polymerase II? |
A. | None of the other answers |
B. | Polyadenylation |
C. | mRNA splicing |
D. | 5' capping |
Answer» B. Polyadenylation | |
23. |
What is the role of the promoter region in the regulation of gene expression? |
A. | Bind negative regulators to inhibit gene expression |
B. | Recruit RNA polymerase and transcription factors to DNA |
C. | Alter gene expression by binding transcription factors |
D. | Allow for alternative splicing and recombination of genetic components |
Answer» C. Alter gene expression by binding transcription factors | |
24. |
Before RNA polymerase can initiate transcription, this protein must bind to it, creating the RNA polymerase holoenzyme and allowing for the initiation of transcription. |
A. | |
B. | B. |
C. | C. |
D. | D. |
Answer» D. D. | |
25. |
Transcription factors that are the first to bind DNA in heterochromatin regions, often promote euchromatin formation, and recruit other transcriptional machinery to promote transcription are best known as which of the following? |
A. | Co-factors |
B. | Pioneer factors |
C. | DNA Polymerases |
D. | RNA holoenzyme |
Answer» C. DNA Polymerases | |
26. |
Which of the following best describes the key function of helicases during transcription? |
A. | Re-annealing of two DNA strands once transcription and translation processes are complete. |
B. | Relief of tension in the DNA strands to prevent breakage. |
C. | Cleaving methyl groups from an unwound DNA strand |
D. | Separation of two strands of DNA; "unwinding" gives polymerases access to the strand. |
Answer» E. | |
27. |
Which of the following subunits of RNA polymerase is solely required for initiation of transcription? |
A. | (alpha) |
B. | (sigma) |
C. | (omega) |
D. | (beta) |
Answer» C. (omega) | |
28. |
Which of the following codons is the mRNA start codon that initiates translation? |
A. | AUG |
B. | UAG |
C. | UGA |
D. | UAA |
Answer» B. UAG | |
29. |
In eukaryotes, which RNA polymerase makes rRNA? |
A. | RNA polymerase IV |
B. | RNA polymerase II |
C. | RNA polymerase I |
D. | RNA polymerase III |
Answer» D. RNA polymerase III | |
30. |
Transcription by E. coli polymerase occurs in |
A. | four phases known as initiation, propagation, elongation and termination |
B. | three phases known as initiation, elongation and termination |
C. | two phases known as initiation and termination |
D. | none of the above |
Answer» C. two phases known as initiation and termination | |
31. |
Rho-dependent termination of transcription in E. coli |
A. | requires ATP |
B. | requires about 50 nucleotides of uncomplexed mRNA |
C. | both (a) and (b) |
D. | removes mRNA and holoenzyme from the DNA |
Answer» D. removes mRNA and holoenzyme from the DNA | |
32. |
Mark the one, which is NOT the transcription inhibitor in eukaryotes. |
A. | Rifampicin |
B. | Acridine dye |
C. | Actinomycin D |
D. | Rho factor |
Answer» E. | |
33. |
Name the one intrinsic terminator of transcription. |
A. | Intercalating agents |
B. | Rho independent |
C. | Rho dependent |
D. | Acridine orange |
Answer» C. Rho dependent | |
34. |
Which of the following transcription termination technique has RNA dependent ATPase activity? |
A. | Intercalating agents |
B. | Rho dependent |
C. | Rho independent |
D. | Rifampcin |
Answer» C. Rho independent | |
35. |
Which of the level of protein structure is incorrectly matched to its description? |
A. | Primary structure is the linear amino acid sequence of a protein |
B. | Tertiary structure is the two-dimensional structure of a protein |
C. | Secondary structure is determined by hydrogen bonding of the amino acid backbone |
D. | Quaternary structure is the three-dimensional structure of a multi-subunit protein |
Answer» C. Secondary structure is determined by hydrogen bonding of the amino acid backbone | |
36. |
The enzyme required for transcription is |
A. | RNAase |
B. | DNA polymerase |
C. | RNA polymerase |
D. | Restriction enzymes |
Answer» D. Restriction enzymes | |
37. |
In prokaryotes what site on the mRNA does the ribosome bind to initiate translation? |
A. | The 3' untranslated region |
B. | The poly adenine tail |
C. | The Shine-Dalgarno sequence |
D. | The promoter |
Answer» D. The promoter | |
38. |
Most translation occurs by a mRNA cap-dependent mechanism, however, translation can occur by cap-independent initiation. One mechanism by which eukaryotic cells can initiate cap-independent translation is by which of the following approaches? |
A. | Poly(A)-binding protein |
B. | None of these |
C. | Internal ribosome entry site |
D. | elF4F initiation complex |
Answer» D. elF4F initiation complex | |
39. |
Which of the following ensures that viral gene translation occurs even when host translation is inhibited? |
A. | Internal Ribosomal Entry Sites (IRES) |
B. | Promoter |
C. | 3' poly-A tail |
D. | 5' guanine cap |
Answer» B. Promoter | |
40. |
Which amino acid is the "start" amino acid in a peptide chain? |
A. | Methionine |
B. | Arginine |
C. | Tyrosine |
D. | Threonine |
Answer» B. Arginine | |
41. |
Which of the following is not a type of modification that can occur after translation? |
A. | Trimming |
B. | Phosphorylation |
C. | Proteasomal degradation |
D. | 5' capping |
Answer» E. | |
42. |
Which of the following is not a phase in translation? |
A. | Initiation |
B. | Termination |
C. | Elongation |
D. | Modification |
Answer» E. | |
43. |
Which of the following most accurately describes the chronological order of ribosome biogenesis in eukaryotes? |
A. | Ribosomal proteins are translated in the cytoplasm and transported to the nucleolus. At the same time, rRNA genes are being rapidly transcribed in the nucleolus. rRNA and ribosomal proteins form the 60S and 40S subunits in the nucleolus and are then transported to the cytoplasm for functional ribosome assembly. |
B. | Ribosomal proteins are translated in the cytoplasm. At the same time, rRNA genes are being rapidly transcribed in the nucleolus. rRNA is transported to the cytoplasm where the rRNA and ribosomal proteins form the 60S and 40S ribosomal subunits. |
C. | Ribosomal proteins are translated in the cytoplasm and transported to the nucleolus. At the same time, rRNA genes are being rapidly transcribed in the cytoplasm. rRNA and ribosomal proteins form the 60S and 40S subunits in the cytoplasm. |
D. | Ribosomal proteins are translated in the nucleous and transported to the cytoplasm. At the same time, rRNA genes are being rapidly transcribed in the nucleolus. rRNA and ribosomal proteins form the 60S and 4 |
Answer» B. Ribosomal proteins are translated in the cytoplasm. At the same time, rRNA genes are being rapidly transcribed in the nucleolus. rRNA is transported to the cytoplasm where the rRNA and ribosomal proteins form the 60S and 40S ribosomal subunits. | |
44. |
How many ribosomal binding sites are there and what are their functions? |
A. | There are three sites. A site binds aminoacyl-tRNA, P site binds peptidyl-tRNA, E site binds free tRNA before ribosomal exit |
B. | There are three sites. A site binds peptidyl-tRNA, P site binds aminoacyl-tRNA, E site binds free tRNA before ribosomal exit |
C. | There are two sites. A site binds free tRNA before ribosomal exit, P site binds aminoacyl-tRNA |
D. | There are three sites. A site binds free tRNA before ribosomal exit, P site binds aminoacyl-tRNA, E site binds peptidyl-tRNA |
Answer» B. There are three sites. A site binds peptidyl-tRNA, P site binds aminoacyl-tRNA, E site binds free tRNA before ribosomal exit | |
45. |
On which of the following molecules could you find an anticodon? |
A. | rRNA |
B. | tRNA |
C. | mRNA |
D. | miRNA |
Answer» C. mRNA | |
46. |
In which of the following organelles does the initial linkage of a sugar for post-translation modification N-linked glycosylation most commonly occur? |
A. | Golgi apparatus |
B. | Endoplasmic reticulum |
C. | Nucleus |
D. | Mitochondria |
Answer» C. Nucleus | |
47. |
Small GTPases are important molecular switches and signaling pathways. What proteins are responsible for promoting the activation of these small GTPases? |
A. | Kinases |
B. | Nicotinamide adenine dinucleotide |
C. | Guanine nucleotide exchange factors |
D. | Phosphatases |
Answer» D. Phosphatases | |
48. |
Which of the following is not an example of post-translational modification? |
A. | Myristoylation |
B. | Polyadenylation |
C. | Alkylation |
D. | Ubiquitination |
Answer» C. Alkylation | |
49. |
The 5' cap on eukaryotic mRNA molecules is recognized by which of the following proteins? |
A. | 40s ribosomal subunit |
B. | eIF4e |
C. | PABP |
D. | RNA polymerase |
Answer» C. PABP | |
50. |
When conducting a stain of chromosomes, certain regions along the chromosome will stain more darkly than the rest. This is due to the fact that these regions are more tightly condensed. What is the functional outcome of having one region more condensed (heterochromatin) than the rest (euchromatin)? |
A. | Heterochromatin is typically not transcribed/transcribed at a lower rate because the tight packing limits accessibility to polymerases. |
B. | Heterochromatin is transcribed at a high frequency because there are a high number of genes in a small area. |
C. | Heterochromatin does not contain any genes and is a structural component of the chromosome. |
D. | Heterochromatin unpacking requires many ATP and is energetically expensive. |
Answer» B. Heterochromatin is transcribed at a high frequency because there are a high number of genes in a small area. | |