Which of the following have been used for cryo-preservation of germplasm with 90% tissue survival and 10%plant regeneration that are used in international exchange of germplasm?

Embryo cultures supplemented with GA3 and coconut milk

A plasmid used to introduce BT toxin into a plant cell

carries an antibiotic resistance gene that makes the plant resistant to antibiotics

causes the plant to produce opines

carries a bacterial gene with a plant promoter

Cross protection against viruses in transgenic plants can be obtained by

inoculating the susceptible strain with the coat proteins of virulent strain

inoculating the susceptible strain of a crop with a mild strain of a virus that helps in developing resistance against more virulent strain

inoculating the susceptible strain with the coat proteins of virulent strain

inoculating the susceptible strain with genes of nucleocapsid

Which of the following gene is responsible for resistance against chilling?

Glycerol 1 phosphate acyl transferase

Sucrose phosphate synthase gene

Two genes from a bacterium (Alcaligenes eutrophus) and a cotton gene produce

a vaccine against the bacterium

Antisense transgenic plants produced fruit that softened

more rapidly than the normal fruit

more slowly than the normal fruit

more rapidly than the normal fruit

seeds of plants modified genetically

somatic embryos encapsulated in suitable matrix

seeds of plants modified genetically

Which of the following are used as selection marker for the cells transformed with Agrobacterium?

Streptomycin phosphotransferase

Microinjection involves

injection of large amount of DNA

injection of DNA into bigger cells

injection with needle having diameter greater than cell diameter

Which of the following is true about Agrobacterium tumefaciens?

It causes crown gall disease of plants

It infects dicotyledonous angiosperms

In the liposome mediated gene transfer in plants, nucleic acids are

protected from nuclease digestion

shows tumorous properties only in presence of bacteria

can be cultivated in vitro in absence of bacteria

retains tumorous properties when cultivated

shows tumorous properties only in presence of bacteria

Microinjection technique of introducing NA is a

combination of physical and biological method

amino acid derivatives found in normal tissues

amino acid derivatives found in tumor tissues

amino acid derivatives found in normal tissues

amino acid derivatives found in both normal as well as tumor tissues

Co-integrating transformation vectors must include a region of homology in

between vector plasmid and Ti-plasmid

Liposomes mediated gene transfer in plants involves

plasmid DNA enclosed in a lipid bag

fusion of liposomes with protoplast

use of polyethylene glycol (PEG)

In a plant tumour cell

complete Ti plasmid is incorporated in plant nuclear DNA

different parts of the Ti-plasmid are incorporated

only a small specific segment of callus T DNA is incorporated

Which of the following is not true about the direct repeats flanking T-DNA?

These are important in integration mechanism

They are conserved between nopaline and octopine Ti-plasmids

These repeats are transferred intact to the plant genome

These are important in integration mechanism

The left segment of octopine T-DNA (TL) is necessary for

enzymes for agropine biosynthesis

Microprojectile method of gene transfer in plants involves delivery of DNA

with the help of micromanipulator

Which of the following is true about T DNA?

Integration of T DNA occurs only at one specific sites in the plant nuclear DNA

Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA

Integration of T DNA occurs only at one specific sites in the plant nuclear DNA

Integration of T DNA occurs at two specific sites in the plant nuclear DNA

Integration of T DNA occurs at one site that may be random in the plant nuclear DNA

Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast?

It is carried out with the help of micromanipulator

The recipient cells are immobilized on artificial support or artificially bound to substarate

It employs needle with diameter greater than cell diameter

The right segment of octopine T-DNA (TR) is necessary for

enzymes for agropine biosynthesis

Opine synthesis is the property

conferred to plant cells when it transformed by Agrobacterium tumefaciens

determined by the bacteria Agrobacterium tumefaciens

Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain

three sets of sequence necessary for gene transfer

one set of sequence necessary for gene transfer

two sets of sequence necessary for gene transfer

three sets of sequence necessary for gene transfer

four sets of sequence necessary for gene transfer

151 + Mcqs in Gene Transfer in Plants in Biotechnology Page 3 McqOptions

101.	Encapsulation is necessary to produce and protect synthetic seeds. The encapsulation is carried out by various types of hydrogels, which are
A.	soluble in water
B.	soluble in organic solvents
C.	insoluble in water
D.	insoluble in organic solvents
Answer» B. soluble in organic solvents

Discussion

102.	The preserved embryoids are termed as
A.	synthetic seeds
B.	semi-synthetic seeds
C.	natural seeds
D.	fermented seeds
Answer» B. semi-synthetic seeds

Discussion

103.	The hybrid embryo can be rescued from damage/collapse by growing it
A.	at low temperature
B.	at low pH
C.	at low water activity
D.	in artificial medium
Answer» E.

Discussion

104.	Which of the following have been used for cryo-preservation of germplasm with 90% tissue survival and 10%plant regeneration that are used in international exchange of germplasm?
A.	Meristem tip cultures
B.	Embryo cultures
C.	Both (a) and (b)
D.	Embryo cultures supplemented with GA3 and coconut milk
Answer» B. Embryo cultures

Discussion

105.	The size of the virulent plasmid of Agrobacterium tumefaciens is
A.	40-80 kb
B.	80-120 kb
C.	140-235 kb
D.	>235 kb
Answer» D. >235 kb

Discussion

106.	Plant tissue culture technique is used to select somaclones that are
A.	resistant to herbicide
B.	not resistant to herbicide
C.	resistant to insecticides
D.	not resistant to insecticides
Answer» B. not resistant to herbicide

Discussion

107.	Which of the following mutant/(s) has/have increased amount of lysine and/or methionine in corn seeds?
A.	Opaque-2
B.	Sugary-1
C.	Flory-2
D.	All of these
Answer» E.

Discussion

108.	A plasmid used to introduce BT toxin into a plant cell
A.	carries an antibiotic resistance gene that makes the plant resistant to antibiotics
B.	causes the plant to produce opines
C.	is toxic for the plant cell
D.	carries a bacterial gene with a plant promoter
Answer» E.

Discussion

109.	Human serum albumin in case of transgenic plant is secreted in
A.	transgenic leaf tissues
B.	transgenic root tissues
C.	is not secreted out of plant
D.	transgenic stem tissues
Answer» B. transgenic root tissues

Discussion

110.	Cross protection against viruses in transgenic plants can be obtained by
A.	inoculating the susceptible strain of a crop with a mild strain of a virus that helps in developing resistance against more virulent strain
B.	inoculating the susceptible strain with the coat proteins of virulent strain
C.	inoculating the susceptible strain with genes of nucleocapsid
D.	any of the above
Answer» B. inoculating the susceptible strain with the coat proteins of virulent strain

Discussion

111.	Which of the following gene is responsible for resistance against chilling?
A.	Glycerol 1 phosphate acyl transferase
B.	Polygalactouranase
C.	ACC deaminase
D.	Sucrose phosphate synthase gene
Answer» B. Polygalactouranase

Discussion

112.	Two genes from a bacterium (Alcaligenes eutrophus) and a cotton gene produce
A.	a plastic
B.	a vaccine against the bacterium
C.	a pharmaceutical product
D.	herbicide resistance
Answer» B. a vaccine against the bacterium

Discussion

113.	Antisense transgenic plants produced fruit that softened
A.	more slowly than the normal fruit
B.	more rapidly than the normal fruit
C.	as the normal fruits
D.	none of these
Answer» B. more rapidly than the normal fruit

Discussion

114.	Given the DNA sequence ATTAAGGC, which sequence would be used by researchers as an antisense molecule?
A.	AUUAAGGC
B.	UAAUUCCG
C.	CGGAATTA
D.	TAATTCCG
Answer» B. UAAUUCCG

Discussion

115.	Synthetic seeds are
A.	artificially synthesized seeds
B.	somatic embryos encapsulated in suitable matrix
C.	seeds of plants modified genetically
D.	none of these
Answer» C. seeds of plants modified genetically

Discussion

116.	Which cell-based plant technology involves the combining of two cells without cell walls from different species?
A.	Clonal propagation
B.	Cybridization
C.	Protoplast fusion
D.	Mutant selection
Answer» D. Mutant selection

Discussion

117.	Opines that are present in crown gall tumour include
A.	octopine
B.	nopaline
C.	agropine
D.	all of these
Answer» E.

Discussion

118.	Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?
A.	pRK 2013
B.	pRN 3
C.	either (a) or (b)
D.	none of these
Answer» D. none of these

Discussion

119.	Which of the following are used as selection marker for the cells transformed with Agrobacterium?
A.	Neomycin phosphotransferase
B.	Streptomycin phosphotransferase
C.	Hygromycin phosphotransferase
D.	any of the above
Answer» E.

Discussion

120.	Plant transformation vectors based on Agrobacterium can generally be divided into
A.	two vectors
B.	four vectors
C.	six vectors
D.	eight vectors
Answer» B. four vectors

Discussion

121.	Vir genes required for the T-DNA transfer and processing are located
A.	on the T-DNA
B.	outside the T-DNA region
C.	on the plant genome
D.	none of these
Answer» C. on the plant genome

Discussion

122.	Microinjection involves
A.	injection of large amount of DNA
B.	injection of DNA into bigger cells
C.	injection with needle having diameter greater than cell diameter
D.	all of the above
Answer» D. all of the above

Discussion

123.	In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by
A.	vir A
B.	vir B
C.	vir C
D.	vir D
Answer» E.

Discussion

124.	Which of the following is true about Agrobacterium tumefaciens?
A.	It causes crown gall disease of plants
B.	It infects gymosperms
C.	It infects dicotyledonous angiosperms
D.	All of the above
Answer» E.

Discussion

125.	In the liposome mediated gene transfer in plants, nucleic acids are
A.	protected from nuclease digestion
B.	stable in liposomes
C.	both (a) and (b)
D.	not stable in liposomes
Answer» D. not stable in liposomes

Discussion

126.	The transfer of intermediate vectors into Agrobacterium are brought about by
A.	transformation
B.	biparental mating
C.	triparental mating
D.	transduction
Answer» D. transduction

Discussion

127.	Commercial sugarcane is heterozygons with germplasm from
A.	1-3 Saccharum species
B.	3-5 Saccharum species
C.	5-7 Saccharum species
D.	7-9 Saccharum species
Answer» C. 5-7 Saccharum species

Discussion

128.	Crown gall tissue
A.	can be cultivated in vitro in absence of bacteria
B.	retains tumorous properties when cultivated
C.	both (a) and (b)
D.	shows tumorous properties only in presence of bacteria
Answer» D. shows tumorous properties only in presence of bacteria

Discussion

129.	The intervention in the communication system of nematodes offer several possibilities of bio control. Which of the following(s) play(s) a very important role in the host recognition?
A.	Protein
B.	Lipid
C.	Carbohydrate moiety
D.	Minerals
Answer» D. Minerals

Discussion

130.	MS medium, is found essential for initial morphogenesis of the immature embryos and to enhance callus development, when supplemented with
A.	GA3
B.	GA3 and coconut milk
C.	adenine
D.	adenine and kinetin
Answer» C. adenine

Discussion

131.	Microinjection technique of introducing NA is a
A.	physical method
B.	biological method
C.	combination of physical and biological method
D.	chemical method
Answer» B. biological method

Discussion

132.	Which of the following govern sporophytic self-incompatibility in Brassica?
A.	Aegilops
B.	Triticum
C.	Both (a) and (b)
D.	S alleles
Answer» E.

Discussion

133.	T-DNA transfer and processing into plant genome requires products of which of the following genes?
A.	vir A,B
B.	vir G,C
C.	vir D,E
D.	All of these
Answer» E.

Discussion

134.	Opines are
A.	amino acid derivatives found in tumor tissues
B.	amino acid derivatives found in normal tissues
C.	amino acid derivatives found in both normal as well as tumor tissues
D.	none of the above
Answer» B. amino acid derivatives found in normal tissues

Discussion

135.	Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into
A.	pBR 322 based plasmid vector
B.	pRK 2013
C.	pRN 3
D.	all of these
Answer» B. pRK 2013

Discussion

136.	Integrated nopaline T-DNA occurs as
A.	single segment
B.	two segments
C.	three segments
D.	four segments
Answer» B. two segments

Discussion

137.	Co-integrating transformation vectors must include a region of homology in
A.	the vector plasmid
B.	the Ti-plasmid
C.	between vector plasmid and Ti-plasmid
D.	none of these
Answer» D. none of these

Discussion

138.	Integrated octopine T DNA occurs as
A.	single segment
B.	two segments
C.	three segments
D.	four segments
Answer» C. three segments

Discussion

139.	Liposomes mediated gene transfer in plants involves
A.	plasmid DNA enclosed in a lipid bag
B.	fusion of liposomes with protoplast
C.	use of polyethylene glycol (PEG)
D.	all of the above
Answer» E.

Discussion

140.	Which of the following plant signal molecules regulate the expression of vir B, C, D and E in case of tobacco?
A.	Acetosyringone
B.	α-hydroxy syringone
C.	Both (a) and (b)
D.	None of these
Answer» D. None of these

Discussion

141.	In a plant tumour cell
A.	complete Ti plasmid is incorporated in plant nuclear DNA
B.	different parts of the Ti-plasmid are incorporated
C.	only a small specific segment of callus T DNA is incorporated
D.	may vary from plant to plant
Answer» D. may vary from plant to plant

Discussion

142.	Which of the following is not true about the direct repeats flanking T-DNA?
A.	They are conserved between nopaline and octopine Ti-plasmids
B.	These repeats are transferred intact to the plant genome
C.	These are important in integration mechanism
D.	all of the above
Answer» C. These are important in integration mechanism

Discussion

143.	The left segment of octopine T-DNA (TL) is necessary for
A.	enzymes for agropine biosynthesis
B.	tumour formation
C.	conjugative transfer
D.	all of these
Answer» C. conjugative transfer

Discussion

144.	Microprojectile method of gene transfer in plants involves delivery of DNA
A.	with the help of micromanipulator
B.	with the help of bolistics
C.	with the help of needles
D.	any of the above
Answer» C. with the help of needles

Discussion

145.	Which of the following genes are constitutively expressed and control the plant induced activation of other vir genes?
A.	vir A and vir G
B.	vir C and vir D
C.	vir B and vir E
D.	vir A and vir B
Answer» B. vir C and vir D

Discussion

146.	Which of the following is true about T DNA?
A.	Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA
B.	Integration of T DNA occurs only at one specific sites in the plant nuclear DNA
C.	Integration of T DNA occurs at two specific sites in the plant nuclear DNA
D.	Integration of T DNA occurs at one site that may be random in the plant nuclear DNA
Answer» B. Integration of T DNA occurs only at one specific sites in the plant nuclear DNA

Discussion

147.	Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast?
A.	It is carried out with the help of micromanipulator
B.	The recipient cells are immobilized on artificial support or artificially bound to substarate
C.	It employs needle with diameter greater than cell diameter
D.	all of the above
Answer» D. all of the above

Discussion

148.	The right segment of octopine T-DNA (TR) is necessary for
A.	enzymes for agropine biosynthesis
B.	tumour formation
C.	conjugative transfer
D.	all of these
Answer» B. tumour formation

Discussion

149.	Opine synthesis is the property
A.	conferred to plant cells when it transformed by Agrobacterium tumefaciens
B.	determined by the bacteria Agrobacterium tumefaciens
C.	both (a) and (b)
D.	of normal plant cells
Answer» D. of normal plant cells

Discussion

150.	Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain
A.	one set of sequence necessary for gene transfer
B.	two sets of sequence necessary for gene transfer
C.	three sets of sequence necessary for gene transfer
D.	four sets of sequence necessary for gene transfer
Answer» C. three sets of sequence necessary for gene transfer

Discussion

Explore topic-wise MCQs in Biotechnology.

Encapsulation is necessary to produce and protect synthetic seeds. The encapsulation is carried out by various types of hydrogels, which are

The preserved embryoids are termed as

The hybrid embryo can be rescued from damage/collapse by growing it

Which of the following have been used for cryo-preservation of germplasm with 90% tissue survival and 10%plant regeneration that are used in international exchange of germplasm?

The size of the virulent plasmid of Agrobacterium tumefaciens is

Plant tissue culture technique is used to select somaclones that are

Which of the following mutant/(s) has/have increased amount of lysine and/or methionine in corn seeds?

A plasmid used to introduce BT toxin into a plant cell

Human serum albumin in case of transgenic plant is secreted in

Cross protection against viruses in transgenic plants can be obtained by

Which of the following gene is responsible for resistance against chilling?

Two genes from a bacterium (Alcaligenes eutrophus) and a cotton gene produce

Antisense transgenic plants produced fruit that softened

Given the DNA sequence ATTAAGGC, which sequence would be used by researchers as an antisense molecule?

Synthetic seeds are

Which cell-based plant technology involves the combining of two cells without cell walls from different species?

Opines that are present in crown gall tumour include

Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?

Which of the following are used as selection marker for the cells transformed with Agrobacterium?

Plant transformation vectors based on Agrobacterium can generally be divided into

Vir genes required for the T-DNA transfer and processing are located

Microinjection involves

In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by

Which of the following is true about Agrobacterium tumefaciens?

In the liposome mediated gene transfer in plants, nucleic acids are

The transfer of intermediate vectors into Agrobacterium are brought about by

Commercial sugarcane is heterozygons with germplasm from

Crown gall tissue

The intervention in the communication system of nematodes offer several possibilities of bio control. Which of the following(s) play(s) a very important role in the host recognition?

MS medium, is found essential for initial morphogenesis of the immature embryos and to enhance callus development, when supplemented with

Microinjection technique of introducing NA is a

Which of the following govern sporophytic self-incompatibility in Brassica?

T-DNA transfer and processing into plant genome requires products of which of the following genes?

Opines are

Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

Integrated nopaline T-DNA occurs as

Co-integrating transformation vectors must include a region of homology in

Integrated octopine T DNA occurs as

Liposomes mediated gene transfer in plants involves

Which of the following plant signal molecules regulate the expression of vir B, C, D and E in case of tobacco?

In a plant tumour cell

Which of the following is not true about the direct repeats flanking T-DNA?

The left segment of octopine T-DNA (TL) is necessary for

Microprojectile method of gene transfer in plants involves delivery of DNA

Which of the following genes are constitutively expressed and control the plant induced activation of other vir genes?

Which of the following is true about T DNA?

Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast?

The right segment of octopine T-DNA (TR) is necessary for

Opine synthesis is the property

Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain