α and β cyclo dextrins are used for

flavour and odour enhancement in foods

removal of undesired compounds like caffeine from foods

Virulence trait of Agrobacterium tumefaciens is borne on

both chromosomal and plasmid DNA

Somaclonal variation method refers to

small number of species having limited commercial potential

combines genetic material from different species

selectively blocks expression of a gene

combines genetic material from different species

Advantage of microprojectile method over microinjection method for gene transfer in plants include

method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall

gene can be transferred to many cells simultaneously

Resistance to L-phosphinothrium in tobacco plants is developed by the transfer of

gene for EPSPS (5-enol-pyruvyl shikimat 3 phosphate synthase)

gene for ALS (acetolactate synthase)

gene for GS (glutamine synthase)

Metabolic interference is a term used to describe a method to metabolize a compound and prevent the synthesis of something that is normally produced. What compound(s) have been targeted for metabolic interference in tomato?

ACC (1-aminocyclopropane-1-carboxylic acid)

are plants that differ in geographical locations

contain foreign genes in their cells

are used to produce human antibodies

are plants that differ in geographical locations

Which of the following statements is true regarding genomics?

Plant genomics lags behind similar efforts in animals and microorganisms

Researchers can make use of genomic information even if the entire genome of an organism is not known

Researchers are busy in trying to determine the genome of the potato plant

Low temperatures induce the expression of many cold-induced genes. Transgenic plants with improved cold tolerance have been produced by

cooling plants using the Peltier effect

expressing the protein that activates expression of these genes all the time in plants

cooling plants using the Peltier effect

expressing a gene for production of antifreeze (ethylene glycol) in plants

increasing evaporative cooling from leaf surfaces

Cytoplast (somatic cell hybridization) is the process in which

two enucleated protoplasts fuse

two protoplast along with their nuclei fuse

a normal protoplast along with an enucleated protoplast fuse

two enucleated protoplasts fuse

Which of the agricultural challenges below cannot be solved with transgenic techniques?

Public concern about safety of synthetic pesticides

Public preference for organic vegetables

Meristem culture of banana enables

elimination of bunch top virus disease

The controversy regarding the use of Bt corn is that it

is potentially harmful to monarch butterflies

is a potential allergen to humans

provides exceptionally low yields of protein products

targets genes in the chloroplast

provides exceptionally low yields of protein products

produces genes that are released in pollen

offers little opportunity for practical use

Transgenic plants with increased tolerance to aluminum have been produced by making plants that

secrete phytosiderophores into the soil

make more metal-binding peptides like phytochelatins

The first field tests were conducted with which of the following genetically altered organism?

Vaccinia virus containing a gene from the rabies virus

Strawberry seedlings sprayed with ice-minus bacteria

Somatic embryoids are

identical with zygotic embryos and with seed coats

identical with zygotic embryos and without seed coats

identical with zygotic embryos and with seed coats

non-identical with zygotic embryos and without seed coats

non-identical with zygotic embryos and with seed coats

In transgenic plants that have been manipulated to express patho-genesis-related (PR) proteins all the time it has been shown that

these provide no protection at all against pathogens

expression of one PR protein provides high levels of protection against many pathogens

expression of two PR proteins provides some protection against a few pathogens

these proteins trigger the production of other antimicrobial defenses

Protoplast fusion technique generally involves

hybridization between two species

isolation, fusion and culturing

hybridization between two species

Establishment of embryogenic cell suspensions with high regeneration potential is a pre-requisite for successful

detection of nucleic acid sequence

restriction of fragment length polymorphisms

Resistance to sulphonyl urea compounds in transgenic tobacco plant has been developed by the transfer of

gene for GS (glutamine synthase)

gene for EPSPS (5-enol-pyruvyl shikimat 3 phosphate synthase)

gene for ALS (acetolactate synthase)

gene for GS (glutamine synthase)

In the meiotic cell divison in daughter cells are produced by two successive division in which

first division is reductional and second is equational

first division is equational, second is reductional

first division is reductional and second is equational

Which of the following has been widely used to provide resistance against plant viruses?

Expression of anti-virus genes in vectors that transmit viruses

Virus resistance genes from bacteria

Expression of virus coat protein genes in transgenic plants

Expression of anti-virus genes in vectors that transmit viruses

Expression of ribonuclease (RNase) genes in host plants

For developing herbicide resistance in transgenic plants which of the following approach/(es) is/are used?

Target molecule is made insensitive to herbicide

A pathway should be introduced that detoxify the herbicide

151 + Mcqs in Gene Transfer in Plants in Biotechnology Page 2 McqOptions

51.	Which of the following constituent/(s) is/are added to help in speedy generation of meristem culture in case of Ipomoea batatas improvement?
A.	Auxins
B.	Cytokinins
C.	Both (a) and (b)
D.	Adenine
Answer» D. Adenine

Discussion

52.	α and β cyclo dextrins are used for
A.	pharmaceutical delivery system
B.	flavour and odour enhancement in foods
C.	removal of undesired compounds like caffeine from foods
D.	all of the above
Answer» E.

Discussion

53.	cDNA clone prepared to stigma mRNA of Brassica oleracea can be used as a probe on DNA digests of segregating populations to recognize
A.	Aegilops
B.	Triticum
C.	Both (a) and (b)
D.	S alleles
Answer» E.

Discussion

54.	Virulence trait of Agrobacterium tumefaciens is borne on
A.	chromosomal DNA
B.	tumour inducing plasmid DNA
C.	both chromosomal and plasmid DNA
D.	cryptic plasmid DNA
Answer» C. both chromosomal and plasmid DNA

Discussion

55.	Somaclonal variation method refers to
A.	heritable changes
B.	genetic isolation
C.	small number of species having limited commercial potential
D.	large number of plant cells
Answer» B. genetic isolation

Discussion

56.	On Ti-plasmid T-region or T-DNA is flanked by a direct repeat of
A.	12 bp
B.	20 bp
C.	25 bp
D.	30 bp
Answer» D. 30 bp

Discussion

57.	Which of the following is the class of hybridization?
A.	Intervarietal
B.	Interspecific
C.	Intergeneric
D.	all of these
Answer» E.

Discussion

58.	Which of the following is not a characteristic of a transgenic crop?
A.	herbicide resistance
B.	Bt insect resistance toxin
C.	increased methionine content
D.	none of these
Answer» E.

Discussion

59.	Antisense technology
A.	selectively blocks expression of a gene
B.	combines genetic material from different species
C.	combines organelles and cells
D.	alters or transfers cells
Answer» B. combines genetic material from different species

Discussion

60.	Cultivated tomato species (Lycopersicon esculentum) has a high degree of resistance towards
A.	Meloidogyne spp.nematodes
B.	L. peruviaanum
C.	both (a) and (b)
D.	Solarium melongena
Answer» C. both (a) and (b)

Discussion

61.	Advantage of microprojectile method over microinjection method for gene transfer in plants include
A.	intact cells are used
B.	method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall
C.	gene can be transferred to many cells simultaneously
D.	all of the above
Answer» E.

Discussion

62.	Resistance to L-phosphinothrium in tobacco plants is developed by the transfer of
A.	gene for EPSPS (5-enol-pyruvyl shikimat 3 phosphate synthase)
B.	gene for ALS (acetolactate synthase)
C.	gene for GS (glutamine synthase)
D.	any of the above
Answer» D. any of the above

Discussion

63.	Avermectins (AVM) are macrocyclic lactones which have potent antihelminthic and insecticidal action. They are generally derived from the mycelia of
A.	Streptomyces avermitilis
B.	B. thrunigiensis
C.	P. flourescens
D.	L. peruvianum
Answer» B. B. thrunigiensis

Discussion

64.	Metabolic interference is a term used to describe a method to metabolize a compound and prevent the synthesis of something that is normally produced. What compound(s) have been targeted for metabolic interference in tomato?
A.	ACC (1-aminocyclopropane-1-carboxylic acid)
B.	SAM (S-adenosylmethionine)
C.	Both (a) and (b)
D.	AOA (aminooxyacetic acid)
Answer» D. AOA (aminooxyacetic acid)

Discussion

65.	Plants containing genes encoding cytokines and blood clotting factors are used in
A.	nutrition improvement
B.	pharmaceutical production
C.	vaccine production
D.	textile production
Answer» C. vaccine production

Discussion

66.	Transgenic plants
A.	contain foreign genes in their cells
B.	are used to produce human antibodies
C.	both (a) and (b)
D.	are plants that differ in geographical locations
Answer» D. are plants that differ in geographical locations

Discussion

67.	Which of the following statements is true regarding genomics?
A.	Plant genomics lags behind similar efforts in animals and microorganisms
B.	Researchers can make use of genomic information even if the entire genome of an organism is not known
C.	Researchers are busy in trying to determine the genome of the potato plant
D.	All of the above
Answer» E.

Discussion

68.	Low temperatures induce the expression of many cold-induced genes. Transgenic plants with improved cold tolerance have been produced by
A.	expressing the protein that activates expression of these genes all the time in plants
B.	cooling plants using the Peltier effect
C.	expressing a gene for production of antifreeze (ethylene glycol) in plants
D.	increasing evaporative cooling from leaf surfaces
Answer» B. cooling plants using the Peltier effect

Discussion

69.	Which of the following self-pollinating plant/(s) tend to be homozygous?
A.	Peas
B.	Tomato
C.	Peach
D.	All of these
Answer» E.

Discussion

70.	Cytoplast (somatic cell hybridization) is the process in which
A.	two protoplast along with their nuclei fuse
B.	a normal protoplast along with an enucleated protoplast fuse
C.	both (a) and (b)
D.	two enucleated protoplasts fuse
Answer» D. two enucleated protoplasts fuse

Discussion

71.	The roots of a plant are converted into drug-producing structures in a process called
A.	microcosm establishment
B.	mibridization
C.	bioremediation
D.	rhizosecretion
Answer» E.

Discussion

72.	Involvement of sugarcane phytoalexins in red-hot resistance has been observed. The phytoalexin compound is reported to be
A.	3-deoxyanthocyanidin
B.	luteolinidin
C.	apigeninidin
D.	5-0 apigeninidin
Answer» B. luteolinidin

Discussion

73.	Agrobacterium tumefaciens is a
A.	gram (+) bacteria
B.	gram (-) bacteria
C.	a fungi
D.	a yeast
Answer» C. a fungi

Discussion

74.	Cultivated tomato species (Lycopersicon esculentum) is highly susceptible to
A.	Meloidogyne spp. nematodes
B.	L. peruviaanum
C.	both (a) and (b)
D.	Solarium melongena
Answer» B. L. peruviaanum

Discussion

75.	In transgenic plants of Arabidipsis thaliana which of the following gene is transferred for the synthesis of PHB (polyhdroxybutyrate)?
A.	Acetyl Co A reductase
B.	Polyhydroxybutyrate synthease
C.	Both (a) and (b)
D.	Pyrophosphorylase
Answer» D. Pyrophosphorylase

Discussion

76.	Which of the agricultural challenges below cannot be solved with transgenic techniques?
A.	Crops are damaged by frost
B.	Crops are killed by a virus
C.	Public concern about safety of synthetic pesticides
D.	Public preference for organic vegetables
Answer» E.

Discussion

77.	Which technique is used to introduce genes into dicots?
A.	electroporation
B.	particle acceleration
C.	microinjection
D.	Ti plasmid infection
Answer» E.

Discussion

78.	The homologous chromosomes follow the process of synopsis in the
A.	leptotene
B.	zygotene
C.	pachytene
D.	diplotene
Answer» C. pachytene

Discussion

79.	Meristem culture of banana enables
A.	rapid multiplication
B.	elimination of bunch top virus disease
C.	both (a) and (b)
D.	slow growth
Answer» D. slow growth

Discussion

80.	α and β cyclo dextrins are produced by
A.	transgenic tomato
B.	transgenic maize
C.	transgenic potato
D.	transgenic wheat
Answer» D. transgenic wheat

Discussion

81.	The controversy regarding the use of Bt corn is that it
A.	is potentially harmful to monarch butterflies
B.	is a potential allergen to humans
C.	both (a) and (b)
D.	can contaminate groundwater
Answer» D. can contaminate groundwater

Discussion

82.	Transplastomics
A.	targets genes in the chloroplast
B.	provides exceptionally low yields of protein products
C.	produces genes that are released in pollen
D.	offers little opportunity for practical use
Answer» B. provides exceptionally low yields of protein products

Discussion

83.	Transgenic plants with increased tolerance to aluminum have been produced by making plants that
A.	secrete phytosiderophores into the soil
B.	make more metal-binding peptides like phytochelatins
C.	bind aluminum to the cell wall
D.	secrete citrate into the soil
Answer» E.

Discussion

84.	In case of incomplete dominance, the phenotypic ratio of a monohybrid cross will be
A.	1:2:1
B.	3:1:1
C.	1:3:1
D.	1:1:2
Answer» B. 3:1:1

Discussion

85.	The first field tests were conducted with which of the following genetically altered organism?
A.	bt Corn
B.	Vaccinia virus containing a gene from the rabies virus
C.	The flavrsavr tomato
D.	Strawberry seedlings sprayed with ice-minus bacteria
Answer» E.

Discussion

86.	Somatic embryoids are
A.	identical with zygotic embryos and without seed coats
B.	identical with zygotic embryos and with seed coats
C.	non-identical with zygotic embryos and without seed coats
D.	non-identical with zygotic embryos and with seed coats
Answer» B. identical with zygotic embryos and with seed coats

Discussion

87.	In transgenic plants that have been manipulated to express patho-genesis-related (PR) proteins all the time it has been shown that
A.	these provide no protection at all against pathogens
B.	expression of one PR protein provides high levels of protection against many pathogens
C.	expression of two PR proteins provides some protection against a few pathogens
D.	these proteins trigger the production of other antimicrobial defenses
Answer» E.

Discussion

88.	Protoplast fusion technique generally involves
A.	isolation only
B.	isolation and fusion
C.	isolation, fusion and culturing
D.	hybridization between two species
Answer» D. hybridization between two species

Discussion

89.	Establishment of embryogenic cell suspensions with high regeneration potential is a pre-requisite for successful
A.	protoplast culture
B.	detection of nucleic acid sequence
C.	restriction of fragment length polymorphisms
D.	microinjection
Answer» B. detection of nucleic acid sequence

Discussion

90.	Resistance to sulphonyl urea compounds in transgenic tobacco plant has been developed by the transfer of
A.	gene for EPSPS (5-enol-pyruvyl shikimat 3 phosphate synthase)
B.	gene for ALS (acetolactate synthase)
C.	gene for GS (glutamine synthase)
D.	any of the above
Answer» C. gene for GS (glutamine synthase)

Discussion

91.	Which cell-based technology endows a cell with increased ability to harness energy?
A.	Chlybridization
B.	Cybridization
C.	Mibridization
D.	Protoplast fusion
Answer» B. Cybridization

Discussion

92.	Which of the following transposable elements are not found in plants?
A.	Ac
B.	Tam3
C.	L1
D.	All are found
Answer» D. All are found

Discussion

93.	In the meiotic cell divison in daughter cells are produced by two successive division in which
A.	first division is equational, second is reductional
B.	first division is reductional and second is equational
C.	both divisions are reductional
D.	both divisions are equational
Answer» B. first division is reductional and second is equational

Discussion

94.	In a __________ protocol, bacteria with engineered abilities to detoxify pollutants are intentionally released in an area.
A.	microcosm establishment
B.	mibridization
C.	bioremediation
D.	rhizosecretion
Answer» D. rhizosecretion

Discussion

95.	If the goal were to create a plant resistant to an insecticide, which cell-based plant technology would be most effective?
A.	Clonal propagation
B.	Cybridization
C.	Protoplast fusion
D.	Mutant selection
Answer» E.

Discussion

96.	Which of the following has been widely used to provide resistance against plant viruses?
A.	Virus resistance genes from bacteria
B.	Expression of virus coat protein genes in transgenic plants
C.	Expression of anti-virus genes in vectors that transmit viruses
D.	Expression of ribonuclease (RNase) genes in host plants
Answer» C. Expression of anti-virus genes in vectors that transmit viruses

Discussion

97.	Which tropical fruit crop has been successfully engineered to be protected against a lethal virus?
A.	Passion fruit
B.	Papaya
C.	Mango
D.	Lychee
Answer» C. Mango

Discussion

98.	Which of the following dies from Ti plasmid infection?
A.	Rice
B.	Corn
C.	Sorghum
D.	All of these
Answer» E.

Discussion

99.	For developing herbicide resistance in transgenic plants which of the following approach/(es) is/are used?
A.	Target molecule is made insensitive to herbicide
B.	Target protein is overproduced
C.	A pathway should be introduced that detoxify the herbicide
D.	All of the above
Answer» E.

Discussion

100.	The production of high quality and uniform embryos has been limited to only
A.	carrot
B.	alfalfa
C.	both (a) and (b)
D.	sandalwood
Answer» D. sandalwood

Discussion

Explore topic-wise MCQs in Biotechnology.

Which of the following constituent/(s) is/are added to help in speedy generation of meristem culture in case of Ipomoea batatas improvement?

α and β cyclo dextrins are used for

cDNA clone prepared to stigma mRNA of Brassica oleracea can be used as a probe on DNA digests of segregating populations to recognize

Virulence trait of Agrobacterium tumefaciens is borne on

Somaclonal variation method refers to

On Ti-plasmid T-region or T-DNA is flanked by a direct repeat of

Which of the following is the class of hybridization?

Which of the following is not a characteristic of a transgenic crop?

Antisense technology

Cultivated tomato species (Lycopersicon esculentum) has a high degree of resistance towards

Advantage of microprojectile method over microinjection method for gene transfer in plants include

Resistance to L-phosphinothrium in tobacco plants is developed by the transfer of

Avermectins (AVM) are macrocyclic lactones which have potent antihelminthic and insecticidal action. They are generally derived from the mycelia of

Metabolic interference is a term used to describe a method to metabolize a compound and prevent the synthesis of something that is normally produced. What compound(s) have been targeted for metabolic interference in tomato?

Plants containing genes encoding cytokines and blood clotting factors are used in

Transgenic plants

Which of the following statements is true regarding genomics?

Low temperatures induce the expression of many cold-induced genes. Transgenic plants with improved cold tolerance have been produced by

Which of the following self-pollinating plant/(s) tend to be homozygous?

Cytoplast (somatic cell hybridization) is the process in which

The roots of a plant are converted into drug-producing structures in a process called

Involvement of sugarcane phytoalexins in red-hot resistance has been observed. The phytoalexin compound is reported to be

Agrobacterium tumefaciens is a

Cultivated tomato species (Lycopersicon esculentum) is highly susceptible to

In transgenic plants of Arabidipsis thaliana which of the following gene is transferred for the synthesis of PHB (polyhdroxybutyrate)?

Which of the agricultural challenges below cannot be solved with transgenic techniques?

Which technique is used to introduce genes into dicots?

The homologous chromosomes follow the process of synopsis in the

Meristem culture of banana enables

α and β cyclo dextrins are produced by

The controversy regarding the use of Bt corn is that it

Transplastomics

Transgenic plants with increased tolerance to aluminum have been produced by making plants that

In case of incomplete dominance, the phenotypic ratio of a monohybrid cross will be

The first field tests were conducted with which of the following genetically altered organism?

Somatic embryoids are

In transgenic plants that have been manipulated to express patho-genesis-related (PR) proteins all the time it has been shown that

Protoplast fusion technique generally involves

Establishment of embryogenic cell suspensions with high regeneration potential is a pre-requisite for successful

Resistance to sulphonyl urea compounds in transgenic tobacco plant has been developed by the transfer of

Which cell-based technology endows a cell with increased ability to harness energy?

Which of the following transposable elements are not found in plants?

In the meiotic cell divison in daughter cells are produced by two successive division in which

In a __________ protocol, bacteria with engineered abilities to detoxify pollutants are intentionally released in an area.

If the goal were to create a plant resistant to an insecticide, which cell-based plant technology would be most effective?

Which of the following has been widely used to provide resistance against plant viruses?

Which tropical fruit crop has been successfully engineered to be protected against a lethal virus?

Which of the following dies from Ti plasmid infection?

For developing herbicide resistance in transgenic plants which of the following approach/(es) is/are used?

The production of high quality and uniform embryos has been limited to only