We know that Beta clamp in bacteria has two subunits and takes the shape of a ring. Which of this will be characteristic of PCNA which is a beta clamp homologue for eukaryotes?

Four subunits forming figure 8 like structure

Four subunits forming a larger ring

You design a plasmid with a gene for Histidine synthesis and a fragment containing ARS. You introduce it yeast culture and try to grow then in Histidine deficit media. What will be your observation?

Normal growth with countable colonies

Rapid growth with many colonies

Normal growth with countable colonies

You design a circular ssDNA with a labeled RNA primer (alpha-P32 labeling). You add polymerase epsilon and required enzymes for replication to the fragment. What will you expect to see on autoradiography after gel electrophoresis?

Single labeled band with some labeling at the bottom region

Two labeled band one much lower than the other with labeling at the bottom region

No band but labeling at the bottom

Two labeled band one much lower than the other with no labeling at the bottom

Explore topic-wise MCQs in Cytogenetics.

This section includes 10 Mcqs, each offering curated multiple-choice questions to sharpen your Cytogenetics knowledge and support exam preparation. Choose a topic below to get started.

1.	What is the function of RFC in eukaryotic replication?
A.	Catalytic subunit
B.	SSB
C.	Clamp loader
D.	Primase
Answer» D. Primase

Discussion

2.	We know that Beta clamp in bacteria has two subunits and takes the shape of a ring. Which of this will be characteristic of PCNA which is a beta clamp homologue for eukaryotes?
A.	Two subunits forming ring
B.	Three subunits forming ring
C.	Four subunits forming figure 8 like structure
D.	Four subunits forming a larger ring
Answer» C. Four subunits forming figure 8 like structure

Discussion

3.	You are given a DNA fragment with a CBD lesion. Which polymerase(s) will you use to replicate this fragment without taking care of the error?a) ζ (zeta), η (eta)b) η (eta), ) θ (theta)c) θ (theta), κ (kappa)d) κ (kappa),θ (thet
A.	ζ (zeta), η (eta)
B.	η (eta), ) θ (theta)
C.	θ (theta), κ (kappa)
D.	κ (kappa),θ (theta)
Answer» C. θ (theta), κ (kappa)

Discussion

4.	Which subunit in MCM is not phosphorylated in MCM after initiation is triggered?
A.	1
B.	2
C.	3
D.	7
Answer» C. 3

Discussion

5.	Deletion of which domain of ARS would give the least replication rate in eukaryotes?
A.	A domain
B.	B1 domain
C.	B2 domain
D.	B3 domain
Answer» B. B1 domain

Discussion

6.	You design a plasmid with a gene for Histidine synthesis and a fragment containing ARS. You introduce it yeast culture and try to grow then in Histidine deficit media. What will be your observation?
A.	No growth
B.	One or two colonies
C.	Rapid growth with many colonies
D.	Normal growth with countable colonies
Answer» D. Normal growth with countable colonies

Discussion

7.	You design a circular ssDNA with a labeled RNA primer (alpha-P32 labeling). You add polymerase epsilon and required enzymes for replication to the fragment. What will you expect to see on autoradiography after gel electrophoresis?
A.	Single labeled band with some labeling at the bottom region
B.	Two labeled band one much lower than the other with labeling at the bottom region
C.	No band but labeling at the bottom
D.	Two labeled band one much lower than the other with no labeling at the bottom
Answer» E.

Discussion

8.	You wanted to find out the importance of the DNA polymerases. So, you mutated the polymerases one by one and checked for survival. In which of these mutant cases will you see least survival rate?
A.	Alpha
B.	Beta
C.	Delta
D.	Eta
Answer» B. Beta

Discussion

9.	Here are names of some factors necessary for prokaryotic replication. Which of these or their homologue is unnecessary for eukaryotes?
A.	Dna G
B.	Dna b
C.	Beta clamp
D.	SSB
Answer» B. Dna b

Discussion

10.	In case of eukaryotes replication initiates at ________
A.	TATA
B.	CpG islets
C.	AUG
D.	ARS
Answer» E.

Discussion

Explore topic-wise MCQs in Cytogenetics.

What is the function of RFC in eukaryotic replication?

We know that Beta clamp in bacteria has two subunits and takes the shape of a ring. Which of this will be characteristic of PCNA which is a beta clamp homologue for eukaryotes?

You are given a DNA fragment with a CBD lesion. Which polymerase(s) will you use to replicate this fragment without taking care of the error?a) ζ (zeta), η (eta)b) η (eta), ) θ (theta)c) θ (theta), κ (kappa)d) κ (kappa),θ (thet

Which subunit in MCM is not phosphorylated in MCM after initiation is triggered?

Deletion of which domain of ARS would give the least replication rate in eukaryotes?

You design a plasmid with a gene for Histidine synthesis and a fragment containing ARS. You introduce it yeast culture and try to grow then in Histidine deficit media. What will be your observation?

You design a circular ssDNA with a labeled RNA primer (alpha-P32 labeling). You add polymerase epsilon and required enzymes for replication to the fragment. What will you expect to see on autoradiography after gel electrophoresis?

You wanted to find out the importance of the DNA polymerases. So, you mutated the polymerases one by one and checked for survival. In which of these mutant cases will you see least survival rate?

Here are names of some factors necessary for prokaryotic replication. Which of these or their homologue is unnecessary for eukaryotes?

In case of eukaryotes replication initiates at ________