Explore topic-wise MCQs in Bioprocess Engineering.

This section includes 13 Mcqs, each offering curated multiple-choice questions to sharpen your Bioprocess Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

1.5 g of enzyme gives a vmax of 3 mol product produced per minute. What is the turnover number for this enzyme?

A. 100 sec<sup>-1</sup>
B. 200 sec<sup>-1</sup>
C. 1000 sec<sup>-1</sup>
D. 2000 sec<sup>-1</sup>
Answer» D. 2000 sec<sup>-1</sup>
Discussion
2.

The concept of induced fit refers to the fact that _______

A. When a substrate binds to an enzyme, the enzyme induces a loss of water (desolvation) from the substrate
B. Substrate binding may induce a conformational change in the enzyme, which then brings catalytic groups into proper orientation
C. Enzyme-substrate binding induces an increase in the reaction entropy, thereby catalyzing the reaction
D. Enzyme specificity is induced by enzyme-substrate binding
Answer» C. Enzyme-substrate binding induces an increase in the reaction entropy, thereby catalyzing the reaction
Discussion
3.

What competes with substrate for active site?

A. Competitive inhibition
B. Uncompetitive inhibition
C. Non-competitive inhibition
D. Pure Non-competitive inhibition
Answer» B. Uncompetitive inhibition
Discussion
4.

Which of the following state represents the condition Initial mixing of E+S, while [ES] builds up .

A. Enzyme is saturated with substrate
B. Pre- Steady state
C. Steady state
D. Steady-state kinetics
Answer» C. Steady state
Discussion
5.

What is the unit of catalytic efficiency kcat/Km?

A. conc<sup>-1</sup> time<sup>-1</sup>
B. conc time
C. conc<sup>-2</sup>time<sup>-2</sup>
D. conc time-1
Answer» B. conc time
Discussion
6.

Km is directly proportional to the rate of enzyme binding?

A. True
B. False
Answer» C.
Discussion
7.

Is vmax affected by enzyme concentration?

A. True
B. False
Answer» B. False
Discussion
8.

What is the unit of vmax?

A. mmol
B. mol/sec
C. mol
D. mol/hr
Answer» C. mol
Discussion
9.

What is the term Km ?

A. Concentration of the enzyme
B. Concentration of the catalyst
C. Concentration of the product
D. Concentration of the substrate
Answer» E.
Discussion
10.

Which plot represents the following equation?
( frac{1}{v} = frac{K_m}{v_{max^s}} + frac{1}{v_{max}} )

A. Line-weaver burk plot
B. Eadie-Hofstee plot
C. Michaelis-menten plot
D. Langmuir plot
Answer» D. Langmuir plot
Discussion
11.

Which plot represents the following equation?
( frac{s}{v} = frac{K_m}{v_{max}} + frac{s}{v_{max}} )

A. Line-weaver burk plot
B. Eadie-Hofstee plot
C. Michaelis-menten plot
D. Langmuir plot
Answer» E.
Discussion
12.

Which of the following plot has the difficulty of extrapolating vmax?

A. Line-weaver burk plot
B. Eadie-Hofstee plot
C. Michaelis-menten plot
D. Langmuir plot
Answer» D. Langmuir plot
Discussion
13.

Which stage is preferred for the estimation of the rate of enzymatic reaction?

A. Initial
B. Mid
C. Final
D. Stationary
Answer» B. Mid
Discussion