Explore topic-wise MCQs in Enzyme Technology.

This section includes 7 Mcqs, each offering curated multiple-choice questions to sharpen your Enzyme Technology knowledge and support exam preparation. Choose a topic below to get started.

1.

Use of thermostable baths, buffers are necessary while performing assays.

A. True
B. False
Answer» B. False
2.

Which of the following is a precaution which must be followed while performing assays?

A. It should be ensured that once steady state is reached, the rate of reaction is constant over a period of interest and proportional to the amount of enzyme added
B. The response should be accurate, reproducible, precise, linear and free from electrical noise
C. The probe must be tiny and biocompatible
D. It should be cheap, small portable and capable of being used by semi-skilled operators
Answer» B. The response should be accurate, reproducible, precise, linear and free from electrical noise
3.

Which of the following precautions must not be followed while performing assays?

A. The substrates, buffers etc., should be of high purity
B. Enzyme preparation should as pure as possible
C. The probe must be tiny and biocompatible
D. Stability of the enzyme during the time taken by assay
Answer» D. Stability of the enzyme during the time taken by assay
4.

The reaction catalyzed by ornithine decarboxylase is an example of which assay?

A. Continuous assay
B. Coupled assay
C. Enzymatic assay technique
D. Discontinuous assay
Answer» C. Enzymatic assay technique
5.

Which of the following assays, there is a convenient change is absorbance?

A. Continuous assay
B. Coupled assay
C. Enzymatic assay technique
D. Discontinuous assay
Answer» B. Coupled assay
6.

Which of the following is not a step involved in discontinuous assay?

A. Taking sample from the reaction mixture at known time
B. Stopping the reaction by inactivating the enzyme
C. Measure the convenient change in absorbance
D. Measuring the amount of product formed
Answer» D. Measuring the amount of product formed
7.

In which of the following assays, there is a stop and sample procedure?

A. Continuous assay
B. Coupled assay
C. Enzymatic assay technique
D. Discontinuous assay
Answer» E.