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This section includes 7 Mcqs, each offering curated multiple-choice questions to sharpen your Enzyme Technology knowledge and support exam preparation. Choose a topic below to get started.
1. |
Use of thermostable baths, buffers are necessary while performing assays. |
A. | True |
B. | False |
Answer» B. False | |
2. |
Which of the following is a precaution which must be followed while performing assays? |
A. | It should be ensured that once steady state is reached, the rate of reaction is constant over a period of interest and proportional to the amount of enzyme added |
B. | The response should be accurate, reproducible, precise, linear and free from electrical noise |
C. | The probe must be tiny and biocompatible |
D. | It should be cheap, small portable and capable of being used by semi-skilled operators |
Answer» B. The response should be accurate, reproducible, precise, linear and free from electrical noise | |
3. |
Which of the following precautions must not be followed while performing assays? |
A. | The substrates, buffers etc., should be of high purity |
B. | Enzyme preparation should as pure as possible |
C. | The probe must be tiny and biocompatible |
D. | Stability of the enzyme during the time taken by assay |
Answer» D. Stability of the enzyme during the time taken by assay | |
4. |
The reaction catalyzed by ornithine decarboxylase is an example of which assay? |
A. | Continuous assay |
B. | Coupled assay |
C. | Enzymatic assay technique |
D. | Discontinuous assay |
Answer» C. Enzymatic assay technique | |
5. |
Which of the following assays, there is a convenient change is absorbance? |
A. | Continuous assay |
B. | Coupled assay |
C. | Enzymatic assay technique |
D. | Discontinuous assay |
Answer» B. Coupled assay | |
6. |
Which of the following is not a step involved in discontinuous assay? |
A. | Taking sample from the reaction mixture at known time |
B. | Stopping the reaction by inactivating the enzyme |
C. | Measure the convenient change in absorbance |
D. | Measuring the amount of product formed |
Answer» D. Measuring the amount of product formed | |
7. |
In which of the following assays, there is a stop and sample procedure? |
A. | Continuous assay |
B. | Coupled assay |
C. | Enzymatic assay technique |
D. | Discontinuous assay |
Answer» E. | |