MCQOPTIONS
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MCQOPTIONS
This section includes 14 Mcqs, each offering curated multiple-choice questions to sharpen your Vector Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
How can identification of recombinants of lambda-gt10 vector be done? |
| A. | tAmpicillin resistance |
| B. | tLac selection |
| C. | tcI gene insertional inactivation |
| D. | tAgar and X-gal plating |
| Answer» D. tAgar and X-gal plating | |
| 2. |
Which property is not associated with a lambda insertion vector? |
| A. | tNon-essential region removed |
| B. | tTwo vector arms ligated together |
| C. | tAt least one restriction site is present |
| D. | tExpression of the gene can be obtained |
| Answer» E. | |
| 3. |
What type of vector is the lambda-gt10? |
| A. | tInsertion vector |
| B. | tReplacement vector |
| C. | tHybrid vector |
| D. | tUnmodified lambda vector |
| Answer» B. tReplacement vector | |
| 4. |
Insertion and replacement vectors are modified vectors of which of the following? |
| A. | tPlasmid |
| B. | tLambda phage |
| C. | tM13 phage |
| D. | tYeast artificial chromosome |
| Answer» C. tM13 phage | |
| 5. |
Why is natural selection used to isolate modified lambda that lacks certain restriction sites? |
| A. | tStrains that lack sites are known |
| B. | tEasier than in vitro mutagenesis |
| C. | tThere are no restriction sites in lambda |
| D. | tNatural selection is less time consuming |
| Answer» B. tEasier than in vitro mutagenesis | |
| 6. |
What is the basic difference between a modified (non-essential regions removed) and an unmodified lambda vector? |
| A. | tGene expression increases |
| B. | tStable infection |
| C. | tNon- lysogenic cycle |
| D. | tStar activity |
| Answer» D. tStar activity | |
| 7. |
Which non-essential region of the lambda phage can be deleted without impairing viability? |
| A. | tProtein coding |
| B. | tPromoter region |
| C. | tIntegration and excision region |
| D. | tTerminator region |
| Answer» D. tTerminator region | |
| 8. |
What is the size limit for in vitro packaging of an unmodified lambda vector? |
| A. | t10 kb |
| B. | t52 kb |
| C. | t100 kb |
| D. | t10 bp |
| Answer» C. t100 kb | |
| 9. |
What is the size of fragments that can be obtained by using a phagemid vector? |
| A. | t1 kb |
| B. | t10 kb |
| C. | t1500 bp |
| D. | t50 kb |
| Answer» C. t1500 bp | |
| 10. |
How can the recombinants of phagemid vector Pembl8 be identified? |
| A. | tAgar plating |
| B. | tAgar + Antibiotic |
| C. | tAgar + X-gal |
| D. | tMinimal media plating |
| Answer» D. tMinimal media plating | |
| 11. |
Why is a helper phage needed when cloning experiments which a hybrid vector such as Pembl8 is done? |
| A. | tEfficient insertion |
| B. | tAttachment to host |
| C. | tTo provide replicative enzymes |
| D. | tStable transformation |
| Answer» D. tStable transformation | |
| 12. |
What does the M13 fragment in a phagemid contain? |
| A. | tBamHI restriction site |
| B. | tSignal sequences |
| C. | tOrigin of replication |
| D. | tPromoter sequence |
| Answer» C. tOrigin of replication | |
| 13. |
What is a phagemid? |
| A. | tA hybrid vector |
| B. | tPhage vector |
| C. | tPlasmid vector |
| D. | tViral vector |
| Answer» B. tPhage vector | |
| 14. |
What is an additional feature of M13mp7? |
| A. | t2 antibiotic resistance genes |
| B. | tBigger size |
| C. | tMultiple cloning sites |
| D. | tSmaller size |
| Answer» D. tSmaller size | |