MCQOPTIONS
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MCQOPTIONS
This section includes 10 Mcqs, each offering curated multiple-choice questions to sharpen your Vector Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
What is the basis of cosmid construction? |
| A. | Origin of replication is vital |
| B. | Only cos sites are required for packaging |
| C. | Promoter sequences for expression |
| D. | Different restriction sites |
| Answer» C. Promoter sequences for expression | |
| 2. |
What are cosmid vectors? |
| A. | Hybrid between phage and plasmid |
| B. | Hybrid between M13 and lambda phage |
| C. | Modified lambda vector |
| D. | Modified M13 vector |
| Answer» B. Hybrid between M13 and lambda phage | |
| 3. |
What is a pre-requisite for cloning experiments with lambda insertion or replacement vectors? |
| A. | Circular form of the vector |
| B. | Small size |
| C. | Helper phage integration |
| D. | Two-phage system |
| Answer» B. Small size | |
| 4. |
Recombinant selection of lambda-EMBL4 can be done on the basis of Spi phenotype. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 5. |
Lambda-EMBL4 is an example of _________ |
| A. | Insertion vector |
| B. | Replacement vector |
| C. | Hybrid vector |
| D. | Mammalian vector |
| Answer» C. Hybrid vector | |
| 6. |
How can recombinant selection for lambda replacement vectors be done? |
| A. | In vitro packaging |
| B. | cI gene inactivation |
| C. | Lac selection |
| D. | Antibiotic resistance |
| Answer» B. cI gene inactivation | |
| 7. |
What is not true in regard to a lambda replacement vector? |
| A. | It contains two/more restriction sites |
| B. | Contains stuffer fragment |
| C. | Recombinant selection by size |
| D. | Carries smaller DNA than insertion vectors |
| Answer» E. | |
| 8. |
What is a “stuffer fragment” in cloning jargon? |
| A. | A replaceable fragment |
| B. | Promoter region |
| C. | Ribosomal binding site |
| D. | Clustered with restriction sites |
| Answer» B. Promoter region | |
| 9. |
How can lambda-ZAPII recombinants be distinguished? |
| A. | Agar + ampicillin plating |
| B. | Agar + tetracycline plating |
| C. | Plaque morphology analysis |
| D. | Agar + X-gal plating |
| Answer» E. | |
| 10. |
Lambda-ZAPII can carry what size of the foreign DNA? |
| A. | 1 kb |
| B. | 5 kb |
| C. | 10 kb |
| D. | 15 kb |
| Answer» D. 15 kb | |