MCQOPTIONS
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This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Vector Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
WHICH_SELECTION_SYSTEM_IS_USED_IN_THE_PUC8_PLASMID??$ |
| A. | Antibiotic selection |
| B. | Lactose selection |
| C. | Auxotrophic mutant selection |
| D. | Plaque morphology selection |
| Answer» C. Auxotrophic mutant selection | |
| 2. |
What additional feature does Pgem3Z has which makes it a suitable vector for in vitro transcription of cloned genes?$ |
| A. | Unique Ori |
| B. | Promoters |
| C. | Clustered cloning sites |
| D. | LacZ’ gene |
| Answer» C. Clustered cloning sites | |
| 3. |
What is the copy number of the pUC8 plasmid vector?$ |
| A. | 5-10 |
| B. | 50-100 |
| C. | 100-200 |
| D. | 500-700 |
| Answer» E. | |
| 4. |
The M13mp2 consists ________ restriction site. |
| A. | BamHI |
| B. | AluI |
| C. | PvuII |
| D. | EcoRI |
| Answer» E. | |
| 5. |
The M13mp1 contains __________ |
| A. | LacZ’ gene |
| B. | Ampicillin Resistance gene |
| C. | Restriction sites |
| D. | Tetracycline resistance gene |
| Answer» B. Ampicillin Resistance gene | |
| 6. |
There is a sequence region in M13 where the foreign DNA can be inserted. What is this sequence called? |
| A. | Inverted repeat |
| B. | Palindromic |
| C. | Intergenic |
| D. | Interstitial |
| Answer» D. Interstitial | |
| 7. |
The size of M13 phage vector is _________ |
| A. | 3 kb |
| B. | 6.4 kb |
| C. | 15.4 kb |
| D. | 20.4 kb |
| Answer» C. 15.4 kb | |
| 8. |
pBR327 is a conjugative plasmid? |
| A. | True |
| B. | False |
| Answer» C. | |
| 9. |
How many sets of antibiotic resistance does the plasmid Pbr322 carry? |
| A. | 1 |
| B. | 3 |
| C. | 2 |
| D. | 4 |
| Answer» D. 4 | |
| 10. |
Size of Pbr322 is _______ |
| A. | 100 kb |
| B. | 10 kb |
| C. | 4.3 kb |
| D. | 1 kb |
| Answer» C. 4.3 kb | |
| 11. |
Which antibiotic resistance is present in pBR322? |
| A. | Ampicillin |
| B. | Kanamycin |
| C. | Lactase |
| D. | Gentamycin |
| Answer» B. Kanamycin | |
| 12. |
Which of the following properties is not taken into account while looking for a suitable vector? |
| A. | Size |
| B. | Parent organism |
| C. | Restriction site |
| D. | Origin of replication |
| Answer» C. Restriction site | |
| 13. |
Bolivar and Rodriguez constructed which vector? |
| A. | Yip7 |
| B. | R6-5 |
| C. | pUC8 |
| D. | Pbr322 |
| Answer» E. | |
| 14. |
Which is an example of a simplest vector (in terms of size)? |
| A. | 2 micron circle |
| B. | Bacteriophage |
| C. | Plasmid |
| D. | YAC |
| Answer» D. YAC | |
| 15. |
Which organism has the highest number of vectors? |
| A. | Yeast |
| B. | Mammalian cells |
| C. | E.coli |
| D. | Fungi |
| Answer» D. Fungi | |