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This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Cell Biology knowledge and support exam preparation. Choose a topic below to get started.
1. |
The process of dedifferentiation in cell culture can give rise to ________________________ |
A. | induced-pluripotent stem cells |
B. | carcinoma cells |
C. | single protoplasts |
D. | fused protoplasts |
Answer» B. carcinoma cells | |
2. |
Under favorable conditions, the protoplasts can grow into a ____________________ |
A. | callus culture |
B. | organ culture |
C. | root culture |
D. | shoot culture |
Answer» B. organ culture | |
3. |
HeLa cells are a cell line. |
A. | True |
B. | False |
Answer» B. False | |
4. |
EDTA binds the ______________ ions. |
A. | magnesium |
B. | iron |
C. | carbon |
D. | calcium |
Answer» E. | |
5. |
In the secondary culture, cells are obtained from _______________________ |
A. | primary culture |
B. | the organism |
C. | organ culture |
D. | phenotypic culture |
Answer» B. the organism | |
6. |
Trypsin is used for dissociating the tissue into single cells. |
A. | True |
B. | False |
Answer» B. False | |
7. |
Silver thiosulphate is added to culture medium as it helps to |
A. | maintain the pH |
B. | remove toxic phenolics from plant cells |
C. | prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition. |
D. | all of the above |
Answer» D. all of the above | |
8. |
What is 'nurse' or conditioned medium? |
A. | It is the media full of growth factors used for the growth of cells |
B. | It is the medium added to nurse the callus culture |
C. | Both (a) and (b) |
D. | It is the liquid medium removed from the suspension of fast growing cells |
Answer» E. | |
9. |
Which of the following is not true about nurse or conditioned medium? |
A. | It is liquid removed from the suspension of fast growing cells |
B. | It contains uncharacterized growth factor released by growing cells |
C. | It is used in the culture of regenerating protoplast |
D. | it is removed aseptically from the culture and is autoclaved before use |
Answer» E. | |
10. |
To maintain the pH of the culture |
A. | organic acid such as citric, fumaric, malic and succinic acid is used |
B. | synthetic buffers such as Tris, MES or HEPS are used |
C. | both (a) and (b) |
D. | ammonium salts are used |
Answer» D. ammonium salts are used | |
11. |
Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media |
A. | utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise |
B. | utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise |
C. | utilization of both ammonium and nitrate result in rise in pH |
D. | utilization of both ammonium and nitrate result in drop in pH |
Answer» B. utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise | |
12. |
Which of the following growth regulator is used to stimulate embryo or shoot development? |
A. | Auxins |
B. | Cytokinins |
C. | Gibberellins |
D. | Brassinosteroids |
Answer» D. Brassinosteroids | |
13. |
Which of the following growth regulator is added for short initiation during plant regeneration from callus? |
A. | Auxins |
B. | Cytokinins |
C. | Gibberellins |
D. | Brassinosteroids |
Answer» C. Gibberellins | |
14. |
Which of the following growth regulator promote cell division? |
A. | Auxins |
B. | Cytokinins |
C. | Gibberellins |
D. | Brassinosteroids |
Answer» C. Gibberellins | |
15. |
Which is the most common carbon source used in the plant cell culture media? |
A. | Sucrose |
B. | Glucose |
C. | Fructose |
D. | Maltose |
Answer» B. Glucose | |