You grow a bacterial culture in a media containing N15 and transfer it to a media with N14. After two rounds of replication you perform a CsCl density gradient centrifugation of the DNA. How many bands will you observe what will be their intensity?

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TuteeHUB · Accepted Answer

Three with middle one more intense than upper and lower

TuteeHUB · Answer

One, very intense

TuteeHUB · Answer

Two, equally intense

TuteeHUB · Answer

Three equally intense

1.	You grow a bacterial culture in a media containing N¹⁵ and transfer it to a media with N¹⁴. After two rounds of replication you perform a CsCl density gradient centrifugation of the DNA. How many bands will you observe what will be their intensity?
A.	One, very intense
B.	Two, equally intense
C.	Three with middle one more intense than upper and lower
D.	Three equally intense
Answer» C. Three with middle one more intense than upper and lower

You grow a bacterial culture in a media containing N¹⁵ and transfer it to a media with N¹⁴. After two rounds of replication you perform a CsCl density gradient centrifugation of the DNA. How many bands will you observe what will be their intensity?

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