Which of the following is important in estimating decimal reduction dose (D value)?

• A. Observations made in the food itself
• B. Desirable to experiment with the largest feasible initial number of cells and to follow their destruction to the lowest level which can be observed within the limits of statistical significance
• C. Observations made in the food itself and desirable to experiment with the largest feasible initial number of cells and to follow their destruction to the lowest level which can be observed within the limits of statistical significance
• D. Neither of the mentioned
• E. ?a) Observations made in the food itselfb) Desirable to experiment with the largest feasible initial number of cells and to follow their destruction to the lowest level which can be observed within the limits of statistical significancec) Observations made in the food itself and desirable to experiment with the largest feasible initial number of cells and to follow their destruction to the lowest level which can be observed within the limits of statistical significanced) Neither of the mentioned