What is the temperature of the reaction mixture in the second (anneali – McqOptions

MCQOPTIONS

1.	What is the temperature of the reaction mixture in the second (annealing) step of PCR?
A.	100-110 C
B.	90-94 C
C.	70-72 C
D.	50-65 C
Answer» E.

Discussion

No Comment Found

Related MCQs

If we start with 10 double-stranded DNA molecules in PCR amplification, then how many molecules of dsDNA would be obtained after 10 cycles of PCR amplification?
What is the temperature of the reaction mixture in the extension/elongation step of PCR?
If we start with a single double-stranded DNA molecule in PCR amplification, then how many molecules of dsDNA would be obtained after 23 cycles of PCR amplification?
Which of the following cannot produce errors in error-prone PCR?
What is the temperature of the reaction mixture in the second (annealing) step of PCR?
In error-prone PCR the Taq polymerase anneals incompatible base-pairs to each other during amplification.
Which of the following technique allows, the initiation of DNA amplification, starting with tiny amounts of the parent molecule, and produces considerable amounts of mutated genes?
What is the temperature of the reaction mixture in the initiation step of PCR?
PCR machine is also known as thermo-cycler.
Which of the following is not supposed to be present in the PCR reaction mixture solution?