MCQOPTIONS
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MCQOPTIONS
This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Protein Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Families of proteins with homologous tertiary structures that have diverged from a common ancestor show amino acid sequence differences. |
| A. | False |
| B. | True |
| Answer» C. | |
| 2. |
Which of the following is not a step of the protein engineering cycle? |
| A. | Gene cloning |
| B. | Protein assay and characterization |
| C. | 2D NMR |
| D. | Mass spectrometry |
| Answer» E. | |
| 3. |
Which of the following is not true for protein engineering? |
| A. | Protein assay is a step in protein engineering |
| B. | It requires a knowledge-based design |
| C. | Preparative biochemistry is performed before protein assay |
| D. | It does not require gene cloning |
| Answer» E. | |
| 4. |
Which of the following technique is of no use in protein engineering? |
| A. | X-ray crystallography |
| B. | Gene cloning |
| C. | Protein sequencing |
| D. | Lyophilization |
| Answer» E. | |
| 5. |
Which of the following step is performed before site-directed mutagenesis? |
| A. | Expression |
| B. | Protein assay |
| C. | Preparative biochemistry |
| D. | Knowledge-based design of novel protein |
| Answer» E. | |
| 6. |
Which step is performed after the protein assay and characterization in the protein engineering cycle? |
| A. | Gene cloning |
| B. | Site-directed mutagenesis |
| C. | Preparative biochemistry |
| D. | Protein-ligand complex analysis |
| Answer» E. | |
| 7. |
Which of the following is the process of developing proteins with the desired function by manipulating the stability and specificity of a protein? |
| A. | Protein amplification |
| B. | Protein assay |
| C. | Protein expression |
| D. | Protein engineering |
| Answer» E. | |
| 8. |
Which of the following step is followed by the 2D NMR step in the protein engineering cycle? |
| A. | Site-directed mutagenesis |
| B. | Gene cloning |
| C. | Protein assay |
| D. | Display of 3D structure on computer graphics |
| Answer» E. | |
| 9. |
Usually, only one cycle is sufficient to reach an optimally designed protein in sufficient quantities. |
| A. | True |
| B. | False |
| Answer» C. | |
| 10. |
Which step is performed after the directed mutagenesis of the gene? |
| A. | Gene recombination |
| B. | 2D NMR |
| C. | Gene editing |
| D. | Expression |
| Answer» E. | |
| 11. |
In the protein engineering cycle, the three-dimensional structure of the protein-ligand complex is defined by which of the following techniques? |
| A. | HPLC |
| B. | Mass spectrometry |
| C. | Optical spectroscopy |
| D. | High-resolution X-ray analysis |
| Answer» E. | |
| 12. |
Which of the following step is performed just after the protein from a natural organism is isolated? |
| A. | 2D NMR studies |
| B. | Gene cloning |
| C. | Site-directed mutagenesis |
| D. | Preparative biochemistry |
| Answer» E. | |
| 13. |
Random mutagenesis followed by selection at the level of the whole organism is a quick process. |
| A. | True |
| B. | False |
| Answer» C. | |
| 14. |
Which of the following is not an application of protein engineering? |
| A. | Synthesis of chimeric proteins |
| B. | Modification of natural proteins |
| C. | Construction of novel proteins |
| D. | Multiplication of natural proteins |
| Answer» E. | |
| 15. |
Which of the following technology offers new possibilities for modification of natural proteins for specific industrial, clinical, or agricultural purposes? |
| A. | Protein structural studies |
| B. | Protein sequencing |
| C. | Protein assay |
| D. | Protein engineering |
| Answer» E. | |