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This section includes 14 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.
1. |
Which of the following statements is true about two-dimensional electrophoresis? |
A. | Separates proteins of identical molecular weight, same pI but different charge |
B. | Separates proteins of different molecular weight and different pI |
C. | Separates proteins of identical molecular weight that differ in pI |
D. | Isoelectric focusing is also termed as two-dimensional electrophoresis |
Answer» D. Isoelectric focusing is also termed as two-dimensional electrophoresis | |
2. |
Which of the following statements is true about affinity chromatography? |
A. | During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted first |
B. | During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted last |
C. | During the separation of a mixture of proteins, the protein which binds to ligand is eluted first |
D. | Unwanted proteins are eluted by ligand solution |
Answer» B. During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted last | |
3. |
The pattern on paper in chromatography is called __________ |
A. | Chroming |
B. | Chroma |
C. | Chromatograph |
D. | Chromatogram |
Answer» E. | |
4. |
Mobile phase can be ___________ |
A. | Only solid |
B. | Only gas |
C. | Solid or liquid |
D. | Liquid or gas |
Answer» E. | |
5. |
The salt which produces salting out effect during extraction of proteins is? |
A. | NH4 SO4 |
B. | (NH4)2 SO4 |
C. | (NH4)3 SO4 |
D. | NaCl |
Answer» C. (NH4)3 SO4 | |
6. |
WHICH_OF_THE_FOLLOWING_STATEMENTS_IS_TRUE_ABOUT_TWO-DIMENSIONAL_ELECTROPHORESIS??$ |
A. | Separates proteins of identical molecular weight, same pI but different charge |
B. | Separates proteins of different molecular weight and different pI |
C. | Separates proteins of identical molecular weight that differ in pI |
D. | Isoelectric focusing is also termed as two-dimensional electrophoresis |
Answer» D. Isoelectric focusing is also termed as two-dimensional electrophoresis | |
7. |
Which_of_the_following_statements_is_false?$ |
A. | The term activity refers to the total units of enzyme in a solution |
B. | The specific activity is a measure of enzyme purity |
C. | Specific activity increases during purification of an enzyme and becomes maximal and constant when the enzyme is pure |
D. | Specific activity decreases during purification of an enzyme and becomes maximal and constant when the enzyme is pure |
Answer» E. | |
8. |
Which of the following statements is true about SDS polyacrylamide chromatography? |
A. | SDS polyacrylamide gel electrophoresis separates proteins on the basis of size |
B. | SDS polyacrylamide gel electrophoresis separates proteins on the basis of charge |
C. | SDS binds to proteins non-covalently with a stoichiometry of around one SDS molecule per three amino acids |
D. | SDS binds to proteins non-covalently with a stoichiometry of around one SDS molecule per one amino acid |
Answer» C. SDS binds to proteins non-covalently with a stoichiometry of around one SDS molecule per three amino acids | |
9. |
Which of the following statements is true about ion-exchange chromatography? |
A. | It separates proteins according to their size |
B. | The column matrix with bound anionic groups is called cationic exchanger |
C. | The column matrix with bound anionic groups is called anionic exchanger |
D. | The column matrix with bound cationic groups is called cationic exchanger |
Answer» C. The column matrix with bound anionic groups is called anionic exchanger | |
10. |
Which of the following statements is true about size-exclusion chromatography? |
A. | During the separation of a mixture of proteins, protein with smallest molecular weight is eluted first |
B. | During the separation of a mixture of proteins, protein with largest molecular weight is eluted first |
C. | During the separation of a mixture of proteins, protein with largest molecular weight is eluted last |
D. | During the separation of a mixture of proteins, protein with largest molecular weight flow around the beads |
Answer» C. During the separation of a mixture of proteins, protein with largest molecular weight is eluted last | |
11. |
Which of the following statements about column chromatography is correct? |
A. | Resolution increases as the length of the column increases |
B. | Mobile phase is a porous solid material with appropriate chemical properties held in the column |
C. | Stationary phase is a buffered solution that percolates through mobile phase |
D. | Large proteins emerge from the column sooner than small ones |
Answer» B. Mobile phase is a porous solid material with appropriate chemical properties held in the column | |
12. |
The pattern on paper in chromatography is called |
A. | Chroming |
B. | Chroma |
C. | Chromatograph |
D. | Chromatogram |
Answer» E. | |
13. |
Mobile phase can be |
A. | Only solid |
B. | Only gas |
C. | Solid or liquid |
D. | Liquid or gas |
Answer» E. | |
14. |
The salt which produces salting out effect during extraction of proteins is |
A. | NH<sub>4</sub> SO<sub>4</sub> |
B. | (NH<sub>4</sub>)<sub>2</sub> SO<sub>4</sub> |
C. | (NH<sub>4</sub>)<sub>3</sub> SO<sub>4</sub> |
D. | NaCl |
Answer» C. (NH<sub>4</sub>)<sub>3</sub> SO<sub>4</sub> | |