Explore topic-wise MCQs in Biochemistry.

This section includes 14 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.

1.

Which of the following statements is true about two-dimensional electrophoresis?

A. Separates proteins of identical molecular weight, same pI but different charge
B. Separates proteins of different molecular weight and different pI
C. Separates proteins of identical molecular weight that differ in pI
D. Isoelectric focusing is also termed as two-dimensional electrophoresis
Answer» D. Isoelectric focusing is also termed as two-dimensional electrophoresis
2.

Which of the following statements is true about affinity chromatography?

A. During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted first
B. During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted last
C. During the separation of a mixture of proteins, the protein which binds to ligand is eluted first
D. Unwanted proteins are eluted by ligand solution
Answer» B. During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted last
3.

The pattern on paper in chromatography is called __________

A. Chroming
B. Chroma
C. Chromatograph
D. Chromatogram
Answer» E.
4.

Mobile phase can be ___________

A. Only solid
B. Only gas
C. Solid or liquid
D. Liquid or gas
Answer» E.
5.

The salt which produces salting out effect during extraction of proteins is?

A. NH4 SO4
B. (NH4)2 SO4
C. (NH4)3 SO4
D. NaCl
Answer» C. (NH4)3 SO4
6.

WHICH_OF_THE_FOLLOWING_STATEMENTS_IS_TRUE_ABOUT_TWO-DIMENSIONAL_ELECTROPHORESIS??$

A. Separates proteins of identical molecular weight, same pI but different charge
B. Separates proteins of different molecular weight and different pI
C. Separates proteins of identical molecular weight that differ in pI
D. Isoelectric focusing is also termed as two-dimensional electrophoresis
Answer» D. Isoelectric focusing is also termed as two-dimensional electrophoresis
7.

Which_of_the_following_statements_is_false?$

A. The term activity refers to the total units of enzyme in a solution
B. The specific activity is a measure of enzyme purity
C. Specific activity increases during purification of an enzyme and becomes maximal and constant when the enzyme is pure
D. Specific activity decreases during purification of an enzyme and becomes maximal and constant when the enzyme is pure
Answer» E.
8.

Which of the following statements is true about SDS polyacrylamide chromatography?

A. SDS polyacrylamide gel electrophoresis separates proteins on the basis of size
B. SDS polyacrylamide gel electrophoresis separates proteins on the basis of charge
C. SDS binds to proteins non-covalently with a stoichiometry of around one SDS molecule per three amino acids
D. SDS binds to proteins non-covalently with a stoichiometry of around one SDS molecule per one amino acid
Answer» C. SDS binds to proteins non-covalently with a stoichiometry of around one SDS molecule per three amino acids
9.

Which of the following statements is true about ion-exchange chromatography?

A. It separates proteins according to their size
B. The column matrix with bound anionic groups is called cationic exchanger
C. The column matrix with bound anionic groups is called anionic exchanger
D. The column matrix with bound cationic groups is called cationic exchanger
Answer» C. The column matrix with bound anionic groups is called anionic exchanger
10.

Which of the following statements is true about size-exclusion chromatography?

A. During the separation of a mixture of proteins, protein with smallest molecular weight is eluted first
B. During the separation of a mixture of proteins, protein with largest molecular weight is eluted first
C. During the separation of a mixture of proteins, protein with largest molecular weight is eluted last
D. During the separation of a mixture of proteins, protein with largest molecular weight flow around the beads
Answer» C. During the separation of a mixture of proteins, protein with largest molecular weight is eluted last
11.

Which of the following statements about column chromatography is correct?

A. Resolution increases as the length of the column increases
B. Mobile phase is a porous solid material with appropriate chemical properties held in the column
C. Stationary phase is a buffered solution that percolates through mobile phase
D. Large proteins emerge from the column sooner than small ones
Answer» B. Mobile phase is a porous solid material with appropriate chemical properties held in the column
12.

The pattern on paper in chromatography is called

A. Chroming
B. Chroma
C. Chromatograph
D. Chromatogram
Answer» E.
13.

Mobile phase can be

A. Only solid
B. Only gas
C. Solid or liquid
D. Liquid or gas
Answer» E.
14.

The salt which produces salting out effect during extraction of proteins is

A. NH<sub>4</sub> SO<sub>4</sub>
B. (NH<sub>4</sub>)<sub>2</sub> SO<sub>4</sub>
C. (NH<sub>4</sub>)<sub>3</sub> SO<sub>4</sub>
D. NaCl
Answer» C. (NH<sub>4</sub>)<sub>3</sub> SO<sub>4</sub>