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				This section includes 76 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.
| 1. | The dissociation constant of H2O at 25° C is | 
| A. | 10-14 M | 
| B. | 1014 M | 
| C. | 10-7 M | 
| D. | 107 M | 
| Answer» B. 1014 M | |
| 2. | Which among the following statements is true about the following chemical reaction?3Fe(s) + 4H₂O → Fe₃O₄ (s) + 4H₂ | 
| A. | Iron metal is getting reduced | 
| B. | Water is acting as a reducing agent | 
| C. | Water is acting as an oxidizing agent | 
| D. | Water is getting oxidized | 
| Answer» D. Water is getting oxidized | |
| 3. | If pH of solution of NaOH is 12.0 the pH of H₂SO₄ solution of same molarity will be? | 
| A. | 2.0 | 
| B. | 12.0 | 
| C. | 1.7 | 
| D. | 10.0387 | 
| Answer» D. 10.0387 | |
| 4. | Calculate the pKₐ of lactic acid, given that when the concentration of lactic acid is 0.010M and the concentration of lactate is 0.087M, the pH is 4.80. | 
| A. | 4.0 | 
| B. | 3.9 | 
| C. | 3.3 | 
| D. | 4.1 | 
| Answer» C. 3.3 | |
| 5. | Which of the following does not react with water? | 
| A. | Barium | 
| B. | Calcium | 
| C. | Beryllium | 
| D. | Strontium | 
| Answer» D. Strontium | |
| 6. | Identify the products formed in the following reaction.CH₃COOCH₃ + H₂O → A + B | 
| A. | CH₃COOH and CH₃OH | 
| B. | CH₃COOOH and CH₄ | 
| C. | CO₂ and C₂H₆ | 
| D. | No reaction | 
| Answer» B. CH₃COOOH and CH₄ | |
| 7. | Which can act as buffer? | 
| A. | NH₄Cl + HCl | 
| B. | CH₃COOH + H₂CO₃ | 
| C. | 40ml of 0.1M NaCN + 20ml of 0.1M HCN | 
| D. | NaCl + NaOH | 
| Answer» D. NaCl + NaOH | |
| 8. | Assertion A: pH of a buffer solution solution does not change on dilution.Reason R: On dilution the ration of concentration of salt and acid (or base) remains unchanged. | 
| A. | A and R are true, R is the correct explanation of A | 
| B. | A and R are true, R is not correct explanation of A | 
| C. | A is true but R is false | 
| D. | A is false but R is true | 
| Answer» B. A and R are true, R is not correct explanation of A | |
| 9. | Hydrolysis reactions are catalyzed by enzymes called ___________ | 
| A. | Oxidoreductase | 
| B. | Ligase | 
| C. | Hydrolase | 
| D. | Isomerase | 
| Answer» D. Isomerase | |
| 10. | Calculate the pH of a mixture of 0.10M acetic acid and 0.20M sodium acetate. The pKₐ of acetic acid is 4.76. | 
| A. | 5.1 | 
| B. | 4.1 | 
| C. | 6.1 | 
| D. | 7.1 | 
| Answer» B. 4.1 | |
| 11. | Which of the following is the correct equation? | 
| A. | CO₂ + H₂O → H₂CO₃ | 
| B. | CO₂ + H₂O → HCO₃ | 
| C. | CO₂ + H₂O → H₃CO₃ | 
| D. | CO₂ + H₂O → H₂CO₂ | 
| Answer» B. CO₂ + H₂O → HCO₃ | |
| 12. | What does it mean when we say that water acts as a “heat buffer”? | 
| A. | It keeps the temperature of an organism relatively constant as the temperature of the surroundings fluctuates | 
| B. | It increases the temperature of an organism as the temperature of the surroundings increases | 
| C. | It decreases the temperature of an organism as the temperature of the surroundings increases | 
| D. | It increases the temperature of an organism as the temperature of the surroundings decreases | 
| Answer» B. It increases the temperature of an organism as the temperature of the surroundings increases | |
| 13. | Consider the following reaction, in the presence of light2H₂O + 2X → O₂ + 2XH₂Which of the following statements is true? | 
| A. | X is electron-accepting specie | 
| B. | X is electron-donating specie | 
| C. | Water is an electron acceptor | 
| D. | Water neither donates nor accepts any electron | 
| Answer» B. X is electron-donating specie | |
| 14. | The buffer capacity is equal to __________ | 
| A. | Δn / ΔpH | 
| B. | pH / Δn | 
| C. | ± 1pKₐ | 
| D. | ± 2pKₐ | 
| Answer» B. pH / Δn | |
| 15. | Which of the following mixture in aqueous solution of equimolar concentration acts as a buffer solution? | 
| A. | HNO₃ + NaOH | 
| B. | H₂SO₄ + KOH | 
| C. | NH₄OH(excess) + HCl | 
| D. | CH₃COOH + NaOH(excess) | 
| Answer» D. CH₃COOH + NaOH(excess) | |
| 16. | Calculate the ratio of the concentrations of acetate and acetic acid required in a buffer system of pH 5.30. | 
| A. | 3.2 | 
| B. | 3.3 | 
| C. | 3.4 | 
| D. | 3.5 | 
| Answer» E. | |
| 17. | Which of the following is not a condensation reaction? | 
| A. | ADP + inorganic phosphate → ATP + H₂O | 
| B. | R-OH + inorganic phosphate → phosphate ester + H₂O | 
| C. | R-COOH + inorganic phosphate → Acyl phosphate + H₂O | 
| D. | 6CO₂ + 6H₂O → C₆H₁₂O₆ + 6CO₂ | 
| Answer» E. | |
| 18. | Tertiary conformation of proteins is maintained by 3 types of bonds namely ionic, hydrogen and ____________ | 
| A. | Sulfide | 
| B. | Disulfide | 
| C. | Covalent | 
| D. | Peptide | 
| Answer» C. Covalent | |
| 19. | What trend does the reactivity of group II elements follow with water? | 
| A. | Increases down the group | 
| B. | Decreases down the group | 
| C. | Does not follow any particular trend | 
| D. | No reaction | 
| Answer» B. Decreases down the group | |
| 20. | Buffer capacity is maximum when __________ | 
| A. | One mole of NH₄Cl is added to two moles of NH₄OH | 
| B. | One mole of NH₄Cl is added to one moles of NH₄OH | 
| C. | One mole of NH₄Cl is added to one mole of NaOH | 
| D. | One mole of NaCl is added to one mole of NaOH | 
| Answer» C. One mole of NH₄Cl is added to one mole of NaOH | |
| 21. | 1M NaCl and 1M HCl are present in an aqueous solution. What is the solution? | 
| A. | Not a buffer solution with pH < 7 | 
| B. | Not a buffer solution with pH > 7 | 
| C. | A buffer solution with pH < 7 | 
| D. | A buffer solution with pH > 7 | 
| Answer» B. Not a buffer solution with pH > 7 | |
| 22. | Which of the following is a hypotonic solution? | 
| A. | 10% dextrose in water | 
| B. | 0.45% sodium chloride | 
| C. | 5% dextrose in 0.9% sodium chloride | 
| D. | Oceans | 
| Answer» C. 5% dextrose in 0.9% sodium chloride | |
| 23. | 1M NaCl and 1M HCl are present in an aqueous solution. The solution is ____________ | 
| A. | Not a buffer solution with pH < 7 | 
| B. | Not a buffer solution with pH > 7 | 
| C. | A buffer solution with pH < 7 | 
| D. | A buffer solution with pH > 7 | 
| Answer» B. Not a buffer solution with pH > 7 | |
| 24. | The bond dissociation energy of hydrogen bonds in a water molecule is ______________ | 
| A. | 470 kJ/mole | 
| B. | 470 J/mole | 
| C. | 23 kJ/mole | 
| D. | 23 J/mole | 
| Answer» D. 23 J/mole | |
| 25. | Which of the following is an isotonic solution? | 
| A. | 0.9% sodium chloride | 
| B. | 0.45% sodium chloride | 
| C. | Oceans | 
| D. | 10% dextrose in water | 
| Answer» B. 0.45% sodium chloride | |
| 26. | The bond dissociation energy of O-H bond in a water molecule is _____________ | 
| A. | 23 kJ/mole | 
| B. | 470 kJ/mole | 
| C. | 470 J/mole | 
| D. | 23 J/mole | 
| Answer» C. 470 J/mole | |
| 27. | Which of the following acids has the lowest pKₐ value? | 
| A. | Acetic acid | 
| B. | Sulphuric acid | 
| C. | Dil.HCl | 
| D. | Oxalic acid | 
| Answer» C. Dil.HCl | |
| 28. | What is the concentration of OH⁻ in a solution with a H⁺ concentration of 1.3×10⁻⁴ M? | 
| A. | 7.7×10⁻¹⁰ M | 
| B. | 7.7×10⁻⁹ M | 
| C. | 7.7×10⁻¹¹ M | 
| D. | 7.7×10⁻¹² M | 
| Answer» D. 7.7×10⁻¹² M | |
| 29. | Which of the following bases has the highest pKₐ value? | 
| A. | NaOH | 
| B. | NaNO₃ | 
| C. | KNO₃ | 
| D. | KCl | 
| Answer» B. NaNO₃ | |
| 30. | K1 and K2 for oxalic acid are 6.5×10⁻² and 6.1×10⁻⁵ respectively. What will be the [OH⁻] in a 0.01M solution of sodium oxalate? | 
| A. | 9.6×10⁻⁶ | 
| B. | 1.4×10⁻¹ | 
| C. | 1.2×10⁻⁶ | 
| D. | 1.3×10⁻⁸ | 
| Answer» D. 1.3×10⁻⁸ | |
| 31. | If pKb for fluoride at 25°c is 10.83, the ionization constant of hydrofluoric acid in water at this temperature is? | 
| A. | 3.52×10⁻³ | 
| B. | 6.75×10⁻⁴ | 
| C. | 5.38×10⁻² | 
| D. | 1.74×10⁻⁵ | 
| Answer» C. 5.38×10⁻² | |
| 32. | The hydrolysis constant of CH₃COONa is given by | 
| A. | Kh = Kw/Ka | 
| B. | Kh = Kw/Kb | 
| C. | Kh = Kw/Ka×Kb | 
| D. | Kh = Ka + Kb | 
| Answer» B. Kh = Kw/Kb | |
| 33. | The degree of ionization does not depend on? | 
| A. | Temperature | 
| B. | Current | 
| C. | Nature of solvent | 
| D. | Concentration | 
| Answer» C. Nature of solvent | |
| 34. | Which of the following acid/base pairs act as natural buffers in living systems? | 
| A. | H₂CO₃/HCO₃¯ | 
| B. | H₂PO₄-/HPO₄²¯ | 
| C. | Histidine+/histidine | 
| D. | All of these | 
| Answer» E. | |
| 35. | What is the concentration, in moles/liter, of the hydrogen ion, if pH of a solution is 7? | 
| A. | 7 | 
| B. | 7 x 10^-7 | 
| C. | 5 x 10^-7 | 
| D. | 1 x 10^-7 | 
| Answer» E. | |
| 36. | Which of the following alcohols would be most soluble in water? | 
| A. | methanol | 
| B. | ethanol | 
| C. | butanol | 
| D. | octanol | 
| Answer» B. ethanol | |
| 37. | Which of the following is a hypertonic solution? | 
| A. | Oceans | 
| B. | Freshwater habitats | 
| C. | 0.45% sodium chloride | 
| D. | Red blood cells placed in fresh water | 
| Answer» B. Freshwater habitats | |
| 38. | The processes i and ii are respectively.i) CuSO₄ + 5H₂O → CuSO₄.5H₂Oii) PCl₃ + 3H₂O → H₃PO₃ + 3HCl | 
| A. | Hydration and dehydration | 
| B. | Hydration and hydrolysis | 
| C. | Hydrolysis and hydrolysis | 
| D. | Hydration and hydration | 
| Answer» C. Hydrolysis and hydrolysis | |
| 39. | Aqueous solution of the detergents are _________ | 
| A. | Neutral | 
| B. | Acidic | 
| C. | Basic | 
| D. | Amphoteric | 
| Answer» D. Amphoteric | |
| 40. | A compound that is capable of forming hydrogen bonds with water | 
| A. | is probably held together by noncovalent bonds | 
| B. | contains at least some polar covalent bonds | 
| C. | should act as a good buffer for acids and bases | 
| D. | does not dissolve well in water | 
| Answer» C. should act as a good buffer for acids and bases | |
| 41. | For an acid buffer solution the pH is 3. The pH can be increased by ____________ | 
| A. | Increasing the concentration of salt | 
| B. | Increasing the concentration of acid | 
| C. | Decreasing the concentration of salt | 
| D. | Independent of concentration of acid & salt | 
| Answer» B. Increasing the concentration of acid | |
| 42. | What is the heat of vaporization of water? | 
| A. | 2260 J/g | 
| B. | 2260 kJ/g | 
| C. | 1260 J/g | 
| D. | 1260 kJ/g | 
| Answer» B. 2260 kJ/g | |
| 43. | If pH of solution of NaOH is 12.0 the pH of H₂SO₄ solution of same molarity will be ____________ | 
| A. | 2.0 | 
| B. | 12.0 | 
| C. | 1.7 | 
| D. | 10.0387 | 
| Answer» D. 10.0387 | |
| 44. | Most of the important functional groups in biological molecules contain | 
| A. | oxygen and/or nitrogen and are acidic | 
| B. | oxygen and a phosphate | 
| C. | nitrogen and a phosphate | 
| D. | oxygen and/or nitrogen and are polar | 
| Answer» E. | |
| 45. | If protein is made exclusively from nonpolar amino acids, then it will this protein interact with water? | 
| A. | be repelled by water | 
| B. | It will be attracted to water | 
| C. | It will form a membrane in water | 
| D. | It will act as a buffer and stabilize the pH | 
| Answer» B. It will be attracted to water | |
| 46. | Identify the triprotic acid from the following. | 
| A. | Carbonic acid | 
| B. | Bicarbonate | 
| C. | Glycine | 
| D. | Phosphoric acid | 
| Answer» E. | |
| 47. | What is the factor that is responsible for salts like NaCl to dissolve in water easily? | 
| A. | Decrease in entropy | 
| B. | Increase in entropy | 
| C. | Increase in enthalpy | 
| D. | Decrease in enthalpy | 
| Answer» C. Increase in enthalpy | |
| 48. | ΔG is negative for which of the following processes? | 
| A. | Melting of ice and evaporation of water | 
| B. | Oxidation of nitrogen | 
| C. | Oxidation of gold | 
| D. | Burning of chlorine | 
| Answer» B. Oxidation of nitrogen | |
| 49. | What makes water a liquid at room temperature? | 
| A. | Hydrogen bonds between water molecules | 
| B. | Covalent bonding | 
| C. | Noncovalent interactions | 
| D. | Van der Waals forces of attraction | 
| Answer» B. Covalent bonding | |
| 50. | ΔH is positive for which of the following processes? | 
| A. | Oxidation of nitrogen | 
| B. | Melting of ice and evaporation of water | 
| C. | Oxidation of gold | 
| D. | Burning of chlorine | 
| Answer» C. Oxidation of gold | |