MCQOPTIONS
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This section includes 10 Mcqs, each offering curated multiple-choice questions to sharpen your Cytogenetics knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
While in vitro transcription your RNA pol is stalled at a position due to mistreatment. Which factor could you act to help the RNA pol overcome the stalled stage? |
| A. | TFIID |
| B. | TFIIF |
| C. | TFIIS |
| D. | TFIIH |
| Answer» D. TFIIH | |
| 2. |
Which of this is untrue about TBP? |
| A. | It is a part of TFIID |
| B. | It makes a kink in the DNA |
| C. | It is orientation independent |
| D. | It is sequence independent |
| Answer» E. | |
| 3. |
The absence of which factor will prevent TFIIH and TFIIE recruitment? |
| A. | F |
| B. | D |
| C. | B |
| D. | A |
| Answer» B. D | |
| 4. |
Which factor stimulated phosphorylation of CTD of RNA polymerase II? |
| A. | TFIIA |
| B. | TFIIF |
| C. | TFIIH |
| D. | TFIIE |
| Answer» E. | |
| 5. |
A mediator helps to connect ___________ |
| A. | Enhancer to DPE |
| B. | Silencer to Enhancer |
| C. | Enhancer to TAF |
| D. | Insulator to silencer |
| Answer» D. Insulator to silencer | |
| 6. |
Which of these is not true for TFIIH? |
| A. | It is a helicase |
| B. | On initiation the RNA pol starts transcribing leaving TFIIH at pre-IC |
| C. | It has kinase activity |
| D. | It is needed in Double Strand Break (DSB) repair |
| Answer» C. It has kinase activity | |
| 7. |
While performing EMSA, in which situation will you see the band highest? |
| A. | You add TFIID, TFIIA and polymerase |
| B. | You add TFIID, TFIIB, TFIIF and polymerase |
| C. | You add TFIID, TFIIA, TFIIF and polymerase |
| D. | You add TFIIA, TFIIB, TFIIF and polymerase |
| Answer» C. You add TFIID, TFIIA, TFIIF and polymerase | |
| 8. |
For performing EMSA what type of gel should be used? |
| A. | Native |
| B. | Denaturing SDS-PAGE |
| C. | Non-denaturing SDS PAGE |
| D. | Zymography |
| Answer» D. Zymography | |
| 9. |
You wanted to study the assembly of preinitiation complex in eukaryotes. This is by the means of protein-protein interaction. Which of these methods you can t absolutely put in use there? |
| A. | Footprinting |
| B. | Crystal structure |
| C. | EMSA |
| D. | Primer extension |
| Answer» E. | |
| 10. |
Which of this is a cis binding element? |
| A. | TAF |
| B. | TBP |
| C. | TFIIF |
| D. | TFIIH |
| Answer» C. TFIIF | |