Explore topic-wise MCQs in Protein Engineering.

This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Protein Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

The rate of enzymatic reactions is studied by which of the following curves?

A. Reactivity curve
B. Monod curve
C. Stability curve
D. Michaelis-Menten curve
Answer» E.
Discussion
2.

The substrates are attracted to the active site by which of the following interactions?

A. Covalent and Vander Waal s interactions
B. Hydrophobic and covalent interactions
C. Vander Waal s interactions only
D. Electrostatic and hydrophobic forces.
Answer» E.
Discussion
3.

Which among the following is highly specific?

A. Pepsin
B. DNase
C. Chymotrypsin
D. Thrombin
Answer» E.
Discussion
4.

Which of the following represents a ternary complex?

A. A complex of three enzymes
B. A complex of three substrates
C. A complex of two enzymes and one substrate
D. A complex of one enzyme and two substrates
Answer» E.
Discussion
5.

Which of the following represents the correct schematic of enzymatic reactions?

A. Enzyme-Product release enzyme-substrate binding catalysis
B. Enzyme-Product release catalysis enzyme-substrate binding
C. Catalysis enzyme-substrate binding enzyme-Product release
D. Enzyme-substrate binding catalysis enzyme-Product release
Answer» E.
Discussion
6.

All enzymes isolated thus far are specific for the type of chemical reaction they catalyze.

A. False
B. True
Answer» C.
Discussion
7.

All enzymes are highly specific.

A. True
B. False
Answer» C.
Discussion
8.

Which of the following is essential to keep separate the many pathways involving hundreds of enzymes, that function during metabolism?

A. Enzyme quantity
B. Enzyme concentration
C. Enzyme purity
D. Enzyme specificity
Answer» E.
Discussion
9.

Only specific molecules can serve as substrates for a specific enzyme.

A. False
B. True
Answer» C.
Discussion
10.

Alignment of sequences of homologous enzymes with different specificities as well as structural information has proven useful as a guide to identifying residues possibly involved in the enzyme-substrate specificity.

A. False
B. True
Answer» C.
Discussion
11.

Which of the following aids in the purification of recombinant proteins?

A. Introduction of gaps
B. Introduction of random cuts
C. Introduction of mutations
D. Introduction of Tags
Answer» E.
Discussion
12.

Cofactor requirements cannot be altered by mutagenesis.

A. True
B. False
Answer» C.
Discussion
13.

Which of the following is an important issue for the production of industrial recombinant proteins?

A. Obtain fragments of proteins
B. Obtain the protein in the desired location
C. Obtain the whole protein molecule
D. Obtain proteins in pure and active form
Answer» E.
Discussion
14.

Binding sites for metal ions often prove useful to regulate enzyme activity.

A. False
B. True
Answer» C.
Discussion
15.

Protein engineering cannot be used to do which of the following changes?

A. Tuning of enzyme specificity
B. Introduction of novel properties
C. Introduction of regulatory sites or switches
D. Completely change the specificity of an enzyme
Answer» E.
Discussion