MCQOPTIONS
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MCQOPTIONS
This section includes 13 Mcqs, each offering curated multiple-choice questions to sharpen your Protein Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
What are the different frequencies obtained as NMR peaks called? |
| A. | Chemical peaks |
| B. | Biological shifts |
| C. | Physical shifts |
| D. | Chemical shifts |
| Answer» E. | |
| 2. |
Protein samples are now routinely enriched with which of the following isotopes to simplify the NMR data? |
| A. | 1H and 31P |
| B. | 31P and 15N |
| C. | 1H and 15N |
| D. | 13C and 15N |
| Answer» E. | |
| 3. |
Which of the following isotopes does not have a magnetic spin? |
| A. | 1H and 15N |
| B. | 1H and 16O |
| C. | 12C and 31P |
| D. | 12C and 16O |
| Answer» E. | |
| 4. |
Which of the following isotopes have a magnetic spin? |
| A. | 2He and 13C |
| B. | 2He and 12C |
| C. | 1H and 12C |
| D. | 1H and 13C |
| Answer» E. | |
| 5. |
Which of the following is not a limitation of NMR spectroscopy?a) Mostly used for small proteins (< 30 kD |
| A. | Mostly used for small proteins (< 30 kDa) |
| B. | Can only be used for proteins that are stable in solution |
| C. | Problem arises with high frequencies (1000 MHz) |
| D. | Cannot detect dynamic motion within proteins |
| Answer» E. | |
| 6. |
NMR spectroscopy cannot be used to study which of the following? |
| A. | Interaction of protein with other molecules |
| B. | Dynamic motion within proteins |
| C. | Protein folding |
| D. | Protein sequence |
| Answer» E. | |
| 7. |
Three-dimensional structural information is obtained primarily from ‘through space’ NMR experiments involving the ‘nuclear Overhauser effect (NOE)’. |
| A. | False |
| B. | True |
| Answer» C. | |
| 8. |
The chemical shift of an atomic nucleus depends on its molecular environment and is different for each atom in a protein molecule. |
| A. | False |
| B. | True |
| Answer» C. | |
| 9. |
Which of the following isotopes has a magnetic spin? |
| A. | 16O |
| B. | 12C |
| C. | 14N |
| D. | 31P |
| Answer» E. | |
| 10. |
Which of the following isotope is NMR inactive? |
| A. | 1H |
| B. | 13C |
| C. | 15N |
| D. | 12C |
| Answer» E. | |
| 11. |
Which of the following is the definitive method to identify monomolecular organic compounds? |
| A. | Mass spectroscopy |
| B. | Infrared spectroscopy |
| C. | X – ray crystallography |
| D. | NMR spectroscopy |
| Answer» E. | |
| 12. |
Which of the following is a spectroscopic technique used to observe local magnetic fields around atomic nuclei? |
| A. | Optical spectroscopy |
| B. | Atomic spectroscopy |
| C. | Massspectroscopy |
| D. | NMR spectroscopy |
| Answer» E. | |
| 13. |
Which of the following is a technique for the determination of the three-dimensional structure of a protein? |
| A. | Gas chromatography |
| B. | Mass spectroscopy |
| C. | Radiotherapy |
| D. | NMR spectroscopy |
| Answer» E. | |