Explore topic-wise MCQs in Protein Engineering.

This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Protein Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

Consider four ions q1, q2, q3 and q4. q1 has a charge +3 and mass of 290 Da, q2 has a charge +2, and mass of 290 Da, q3 has a charge +3 and mass of 390 Da, and q4 has a charge +2 and mass of 390 Da. Which one of these ions has a maximum m/z ratio?

A. q1
B. q2
C. q3
D. q4
Answer» E.
2.

Which of the following lasers can be used for laser radiation in MALDI?

A. Infrared lasers
B. Greenlight lasers
C. Red light lasers
D. Ultraviolet lasers
Answer» E.
3.

Which of the following provides higher resolution in MALDI-TOF?

A. Blocker
B. Channel
C. Transmitter
D. Reflector
Answer» E.
4.

Which of the following charge has the lowest m/z ratio?Given data: q1 has a charge +1 and mass of 800 Da,q2 has a charge +3 and mass of 3900 Da,q3 has a charge +2 and mass of 2970 Da,q4 has a charge +4 and a mass of 3480 Da.

A. q4
B. q3
C. q2
D. q1
Answer» E.
5.

Which of the following is the correct equation of the time of flight?

A. t=(\(\frac{m}{2q}\))0.5 x l
B. t=(\(\frac{m}{2q}\))2.0 x l
C. t=(\(\frac{m}{2v^{\circ}}\))2.0 x l
D. t=(\(\frac{m}{2v^{\circ}q}\))0.5 x l
Answer» E.
6.

Which of the following cannot be used as a MALDI matrix compound?

A. α-Cyano-4-hydroxycinnamic acid
B. Sinapinic acid
C. 3-Hydroxypicolinic acid
D. 2,3-Dihydroxybenzoic acid
Answer» E.
7.

If a charge has a mass of 3 kg and it is traveling at a speed of 10 m/s in-flight tube, then what will be it’s kinetic energy?

A. 300 J
B. 90 J
C. 100 J
D. 150 J
Answer» E.
8.

In TOF, ions of lower mass are accelerated to lower velocities and reach the detector last.

A. True
B. False
Answer» C.
9.

Which of the following type of mass analyzer uses a magnetic field and cyclotron motion to trap ions?

A. Quadrupole
B. Orbitrap
C. TOF
D. Fourier transform ion cyclotron resonance
Answer» E.
10.

What is the relation of time of flight with the molecular mass of the ion in the TOF mass analyzer?

A. Time of flight is inversely proportional to the square root of the molecular mass of the ion
B. Time of flight is inversely proportional to the molecular mass of the ion
C. Time of flight is directly proportional to the molecular mass of the ion
D. Time of flight is directly proportional to the square root of the molecular mass of the ion
Answer» E.
11.

In the TOF analyzer, all the ions are accelerated at the same velocity.

A. True
B. False
Answer» C.
12.

Which of the following ionization method uses aromatic compounds and organic solvents to achieve ionization?

A. ESI
B. Chemical ionization
C. FAB
D. MALDI
Answer» E.
13.

Sublimation occurs in which of the following ionization methods?

A. ESI
B. FAB
C. Chemical Ionization
D. MALDI
Answer» E.
14.

Which of the following ionization technique uses a short laser pulse to achieve ionization?

A. ESI
B. Chemical Ionization
C. FAB
D. MALDI
Answer» E.
15.

Which mass analyzer is most frequently used with MALDI?

A. Magnetic sector
B. Quadrupole
C. Ion trap
D. TOF
Answer» E.