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This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Protein Engineering knowledge and support exam preparation. Choose a topic below to get started.
1. |
Consider four ions q1, q2, q3 and q4. q1 has a charge +3 and mass of 290 Da, q2 has a charge +2, and mass of 290 Da, q3 has a charge +3 and mass of 390 Da, and q4 has a charge +2 and mass of 390 Da. Which one of these ions has a maximum m/z ratio? |
A. | q1 |
B. | q2 |
C. | q3 |
D. | q4 |
Answer» E. | |
2. |
Which of the following lasers can be used for laser radiation in MALDI? |
A. | Infrared lasers |
B. | Greenlight lasers |
C. | Red light lasers |
D. | Ultraviolet lasers |
Answer» E. | |
3. |
Which of the following provides higher resolution in MALDI-TOF? |
A. | Blocker |
B. | Channel |
C. | Transmitter |
D. | Reflector |
Answer» E. | |
4. |
Which of the following charge has the lowest m/z ratio?Given data: q1 has a charge +1 and mass of 800 Da,q2 has a charge +3 and mass of 3900 Da,q3 has a charge +2 and mass of 2970 Da,q4 has a charge +4 and a mass of 3480 Da. |
A. | q4 |
B. | q3 |
C. | q2 |
D. | q1 |
Answer» E. | |
5. |
Which of the following is the correct equation of the time of flight? |
A. | t=(\(\frac{m}{2q}\))0.5 x l |
B. | t=(\(\frac{m}{2q}\))2.0 x l |
C. | t=(\(\frac{m}{2v^{\circ}}\))2.0 x l |
D. | t=(\(\frac{m}{2v^{\circ}q}\))0.5 x l |
Answer» E. | |
6. |
Which of the following cannot be used as a MALDI matrix compound? |
A. | α-Cyano-4-hydroxycinnamic acid |
B. | Sinapinic acid |
C. | 3-Hydroxypicolinic acid |
D. | 2,3-Dihydroxybenzoic acid |
Answer» E. | |
7. |
If a charge has a mass of 3 kg and it is traveling at a speed of 10 m/s in-flight tube, then what will be it’s kinetic energy? |
A. | 300 J |
B. | 90 J |
C. | 100 J |
D. | 150 J |
Answer» E. | |
8. |
In TOF, ions of lower mass are accelerated to lower velocities and reach the detector last. |
A. | True |
B. | False |
Answer» C. | |
9. |
Which of the following type of mass analyzer uses a magnetic field and cyclotron motion to trap ions? |
A. | Quadrupole |
B. | Orbitrap |
C. | TOF |
D. | Fourier transform ion cyclotron resonance |
Answer» E. | |
10. |
What is the relation of time of flight with the molecular mass of the ion in the TOF mass analyzer? |
A. | Time of flight is inversely proportional to the square root of the molecular mass of the ion |
B. | Time of flight is inversely proportional to the molecular mass of the ion |
C. | Time of flight is directly proportional to the molecular mass of the ion |
D. | Time of flight is directly proportional to the square root of the molecular mass of the ion |
Answer» E. | |
11. |
In the TOF analyzer, all the ions are accelerated at the same velocity. |
A. | True |
B. | False |
Answer» C. | |
12. |
Which of the following ionization method uses aromatic compounds and organic solvents to achieve ionization? |
A. | ESI |
B. | Chemical ionization |
C. | FAB |
D. | MALDI |
Answer» E. | |
13. |
Sublimation occurs in which of the following ionization methods? |
A. | ESI |
B. | FAB |
C. | Chemical Ionization |
D. | MALDI |
Answer» E. | |
14. |
Which of the following ionization technique uses a short laser pulse to achieve ionization? |
A. | ESI |
B. | Chemical Ionization |
C. | FAB |
D. | MALDI |
Answer» E. | |
15. |
Which mass analyzer is most frequently used with MALDI? |
A. | Magnetic sector |
B. | Quadrupole |
C. | Ion trap |
D. | TOF |
Answer» E. | |