MCQOPTIONS
Saved Bookmarks
MCQOPTIONS
This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Protein Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Sometimes intact charged protein generated by ESI is introduced into the mass analyzer and is subjected to gas-phase fragmentation. What is this type of approach called? |
| A. | Fragmentation strategy of protein analysis |
| B. | Left-to-right strategy of protein analysis |
| C. | Bottom-up strategy of protein analysis |
| D. | Top-down strategy of protein analysis |
| Answer» E. | |
| 2. |
ESI-MS can successfully detect post-translational modifications and mutations. |
| A. | False |
| B. | True |
| Answer» C. | |
| 3. |
Which of the following is not an application of ESI-MS? |
| A. | Protein identification and characterization |
| B. | In the clinical laboratory |
| C. | Studying non-covalent interactions |
| D. | Studying covalent interactions |
| Answer» E. | |
| 4. |
In which of the following techniques the gaseous ions are subjected to two or more sequential stages of mass analysis according to the m/z ratio? |
| A. | NMR spectroscopy |
| B. | X-ray crystallography |
| C. | Mass spectrometry |
| D. | Tandem mass spectrometry |
| Answer» E. | |
| 5. |
In ESI-MS vacuum should be maintained in which of the following components? |
| A. | Detector only |
| B. | Mass analyzer only |
| C. | Ion source and mass analyzer |
| D. | Mass analyzer and detector |
| Answer» E. | |
| 6. |
Which of the following is not a component of ESI-MS mass spectrometry? |
| A. | Electrospray ionizer |
| B. | Detector |
| C. | Mass analyzer |
| D. | MALDI |
| Answer» E. | |
| 7. |
Who is the inventor of ESI-MS? |
| A. | Benjamin Franklin |
| B. | Franz H. Kemp |
| C. | Michael Barber |
| D. | John B. Fenn |
| Answer» E. | |
| 8. |
Generally in ESI, very little residual energy is retained by the analyte, and no fragmentation occurs upon ionization. |
| A. | False |
| B. | True |
| Answer» C. | |
| 9. |
Which of the following ionization technique was able to overcome the propensity of the analyte fragmentation? |
| A. | Electron ionization |
| B. | FAB |
| C. | Chemical ionization |
| D. | ESI |
| Answer» E. | |
| 10. |
What is ESI is also known? |
| A. | Standard Pressure Ionization (SPI) |
| B. | High-Pressure Ionization (HPI) |
| C. | Low-Pressure Ionization (LPI) |
| D. | Atmospheric Pressure Ionization (API) |
| Answer» E. | |
| 11. |
The ions created in ESI are accelerated towards mass analyzer depending upon which criteria of ions? |
| A. | Mass of the ion |
| B. | Charge on the ion |
| C. | Weight of the ion |
| D. | Mass and charge of the ion |
| Answer» E. | |
| 12. |
What is the essential principle in ESI? |
| A. | Solubilization |
| B. | Nucleation |
| C. | Sublimation |
| D. | Nebulisation |
| Answer» E. | |
| 13. |
ESI technique does not allow the analysis of large (underivatized) biomolecules, such as proteins and DNA. |
| A. | True |
| B. | False |
| Answer» C. | |
| 14. |
ESI is not a soft ionization technique. |
| A. | True |
| B. | False |
| Answer» C. | |
| 15. |
Which of the following ionization techniques involves the production of ions by spraying a solution of the analyte into an electric field? |
| A. | CI |
| B. | MALDI |
| C. | FAB |
| D. | ESI |
| Answer» E. | |