MCQOPTIONS
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MCQOPTIONS
This section includes 19 Mcqs, each offering curated multiple-choice questions to sharpen your Gene Manipulation knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which DNA are repaired at the site of mismatch? |
| A. | Long |
| B. | Short |
| C. | Degraded |
| D. | Unmethylated |
| Answer» E. | |
| 2. |
The repair system of E.coli is ___________________ |
| A. | Lacking |
| B. | Cysteine-directed |
| C. | Methyl-directed |
| D. | Mutated |
| Answer» D. Mutated | |
| 3. |
Contamination in heteroduplex molecules can be removed by __________________ |
| A. | Gel electrophoresis |
| B. | PCR |
| C. | Chromatography |
| D. | Distillation |
| Answer» D. Distillation | |
| 4. |
The use of high-fidelity DNA polymerases has minimized the problem of ____________ mutations. |
| A. | Internal |
| B. | Site-directed |
| C. | Extraneous |
| D. | Point |
| Answer» D. Point | |
| 5. |
CONTAMINATION_IN_HETERODUPLEX_MOLECULES_CAN_BE_REMOVED_BY___________________?$ |
| A. | Gel electrophoresis |
| B. | PCR |
| C. | Chromatography |
| D. | Distillation |
| Answer» D. Distillation | |
| 6. |
Which DNA are repaired at the site of mismatch?$ |
| A. | Long |
| B. | Short |
| C. | Degraded |
| D. | Unmethylated |
| Answer» E. | |
| 7. |
The_repair_system_of_E.coli_is____________________$ |
| A. | Lacking |
| B. | Cysteine-directed |
| C. | Methyl-directed |
| D. | Mutated |
| Answer» D. Mutated | |
| 8. |
Which kind of DNA are easier to prepare for PCR mutagenesis? |
| A. | Linear |
| B. | Circular |
| C. | Single-stranded |
| D. | Double-stranded |
| Answer» E. | |
| 9. |
All the primer extension methods of mutagenesis require _____________ template. |
| A. | Double-stranded |
| B. | Degraded |
| C. | Single-stranded |
| D. | RNA |
| Answer» D. RNA | |
| 10. |
The mutated strains mutL, mutS, and mutH prevent the methyl-directed repair of mismatches. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 11. |
Which of the following mutations are not used to overcome problems associated with the mismatch repair system? |
| A. | MutL |
| B. | MutS |
| C. | MutH |
| D. | MutE |
| Answer» E. | |
| 12. |
The use of high-fidelity DNA polymerases has minimized the problem of ____________ mutations? |
| A. | Internal |
| B. | Site-directed |
| C. | Extraneous |
| D. | Point |
| Answer» D. Point | |
| 13. |
Clones can be screened using a _____________ |
| A. | PCR |
| B. | Suppressor |
| C. | Probe |
| D. | Promoter |
| Answer» D. Promoter | |
| 14. |
The synthetic oligonucleotide __________ the DNA synthesis. |
| A. | Primes |
| B. | Shortens |
| C. | Lengthens |
| D. | Degrades |
| Answer» B. Shortens | |
| 15. |
For single-primer method the DNA must be __________ |
| A. | Long |
| B. | Short |
| C. | Double-stranded |
| D. | Single-stranded |
| Answer» E. | |
| 16. |
When was the first method of site-directed mutagenesis developed? |
| A. | 1940 |
| B. | 1970 |
| C. | 1980 |
| D. | 1950 |
| Answer» D. 1950 | |
| 17. |
Creation of mutant proteins with novel properties is called ____________ |
| A. | Cloning |
| B. | Protein engineering |
| C. | Mutagenesis |
| D. | Sequencing |
| Answer» C. Mutagenesis | |
| 18. |
Site-directed mutagenesis is changing a given base in the cloned DNA. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 19. |
Mutagens are physical or _________ agents. |
| A. | Chemical |
| B. | Mechanical |
| C. | Hybrid |
| D. | Exogenous |
| Answer» B. Mechanical | |