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This section includes 20 Mcqs, each offering curated multiple-choice questions to sharpen your Gene Manipulation knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The ‘Charon series’ belongs to series of ____________ |
| A. | Genes |
| B. | Vectors |
| C. | Hosts |
| D. | Enzymes |
| Answer» C. Hosts | |
| 2. |
Linkers are required when using enzymes HaeIII and AluI. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 3. |
HaeIII and AluI have ___________ recognition sites. |
| A. | Different |
| B. | Similar |
| C. | Short |
| D. | Unrecognizable |
| Answer» B. Similar | |
| 4. |
Which kind of packing is done for the fragmented genes? |
| A. | In vivo |
| B. | Population |
| C. | Group |
| D. | In vitro |
| Answer» E. | |
| 5. |
The restriction enzymes used by Maniatis had recognition site of ____ nucleotides. |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» E. | |
| 6. |
THE_RESTRICTION_ENZYMES_USED_BY_MANIATIS_HAD_RECOGNITION_SITE_OF______NUCLEOTIDES.?$ |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» E. | |
| 7. |
HaeIII and AluI have ___________ recognition sites.$ |
| A. | Different |
| B. | Similar |
| C. | Short |
| D. | Unrecognizable |
| Answer» B. Similar | |
| 8. |
Which_kind_of_packing_is_done_for_the_fragmented_genes?$ |
| A. | In vivo |
| B. | Population |
| C. | Group |
| D. | In vitro |
| Answer» E. | |
| 9. |
Lambda EMBL3 is a ____________ |
| A. | Gene |
| B. | Phage |
| C. | Phage Vector |
| D. | Protein |
| Answer» D. Protein | |
| 10. |
Sau3AI is a _______ restriction endonuclease. |
| A. | Single |
| B. | Double |
| C. | Hybrid |
| D. | Weak |
| Answer» B. Double | |
| 11. |
The ‘Charon series’ belongs to series of ____________$ |
| A. | Genes |
| B. | Vectors |
| C. | Hosts |
| D. | Enzymes |
| Answer» C. Hosts | |
| 12. |
Linkers_are_required_when_using_enzymes_HaeIII_and_AluI. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 13. |
In the strategy devised by Maniatis, how many restriction enzymes were used? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 14. |
The Clarke and Carbon formula relates the ____________ of including a DNA fragment in a random library. |
| A. | Probability |
| B. | Effects |
| C. | Temperature change |
| D. | Vector requirement |
| Answer» B. Effects | |
| 15. |
If a gene is large, it may be cut more than once by an endonuclease. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 16. |
A genomic library is a collection of _____________ |
| A. | Genes |
| B. | Proteins |
| C. | Vectors |
| D. | Recombinants |
| Answer» E. | |
| 17. |
What is the approximate size of fragments given off by EcoR1? |
| A. | 1 kb |
| B. | 2 kb |
| C. | 3 kb |
| D. | 4 kb |
| Answer» E. | |
| 18. |
Which DNA is restricted to making a genomic library? |
| A. | Genomic |
| B. | Plasmid |
| C. | Phage |
| D. | Plant |
| Answer» B. Plasmid | |
| 19. |
Genomic library construction is concerned with ___________ |
| A. | Gene isolation |
| B. | Protein production |
| C. | Antibiotics |
| D. | Regeneration |
| Answer» B. Protein production | |
| 20. |
The first genomic libraries were cloned in _______________ |
| A. | Plasmid |
| B. | Bacteria |
| C. | Human |
| D. | Plants |
| Answer» B. Bacteria | |