MCQOPTIONS
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MCQOPTIONS
This section includes 242 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.
| 51. |
Which of the following temperature best suits for the growth and multiplication of transformed cells? |
| A. | 35°C |
| B. | 36°C |
| C. | 37°C |
| D. | 30°C |
| Answer» D. 30°C | |
| 52. |
cDNA, a term used in recombinant DNA technology means ____________ |
| A. | Competitive DNA |
| B. | Chemical DNA |
| C. | Complex DNA |
| D. | Complementary DNA |
| Answer» E. | |
| 53. |
Which of the following is used to transfer genes in plants? |
| A. | Ti plasmid |
| B. | pBR 322 |
| C. | EcorI |
| D. | pUC 18 |
| Answer» B. pBR 322 | |
| 54. |
An expression vector |
| A. | always contains an origin of replication |
| B. | usually contains a gene that confers antibiotic resistance to the bacterial host |
| C. | always contains DNA segments for the regulation of mRNA production |
| D. | all of the above |
| Answer» E. | |
| 55. |
What is the goal of structural proteomics project? |
| A. | To crystallize and determine the structure of as many proteins as possible, in many cases with little or no existing information about protein function |
| B. | To identify and sequence of all the genes present in the human body |
| C. | To introduce new genes to human beings |
| D. | To remove disease causing genes from humans |
| Answer» B. To identify and sequence of all the genes present in the human body | |
| 56. |
Where does a restriction endonuclease cuts DNA? |
| A. | Ligation site |
| B. | Recognition sequence |
| C. | Ori |
| D. | Replication site |
| Answer» C. Ori | |
| 57. |
RNAi stands for |
| A. | RNA inducer |
| B. | RNA interference |
| C. | RNA intron |
| D. | RNA insertion |
| Answer» C. RNA intron | |
| 58. |
Which of the following bacterial operon is not controlled by attenuation? |
| A. | Arabinose |
| B. | Tryptophan |
| C. | Leucine |
| D. | Histidine |
| Answer» B. Tryptophan | |
| 59. |
Attenuator sequences base-pair to form a stem-and-loop structure rich in |
| A. | A = T |
| B. | G ≡ C |
| C. | A = C |
| D. | G = C |
| Answer» C. A = C | |
| 60. |
How are expression vectors useful? |
| A. | To produce protein products |
| B. | Used for genomic libraries |
| C. | Used for chromosome synthesis |
| D. | Used for finger printing |
| Answer» B. Used for genomic libraries | |
| 61. |
Isoschizomers recognize |
| A. | same recognition sequence but different recognition site |
| B. | same recognition site and recognition sequence |
| C. | same recognition site and different recognition sequence |
| D. | different recognition site and different recognition sequence |
| Answer» C. same recognition site and different recognition sequence | |
| 62. |
Which of the following is true about plasmids? |
| A. | They are present in all organisms |
| B. | They are present in bacteria only |
| C. | They are present in bacteria and phages |
| D. | They are present in plants and animals |
| Answer» D. They are present in plants and animals | |
| 63. |
Site directed mutagenesis facilitated research on __________ |
| A. | Carbohydrates |
| B. | Proteins |
| C. | Lipids |
| D. | Fats |
| Answer» C. Lipids | |
| 64. |
Which of the following is the correct order of organization of genetic material from largest to smallest? |
| A. | Genome, chromosome, gene, nucleotide |
| B. | Nucleotide, gene, chromosome, genome |
| C. | Gene, nucleotide, chromosome, genome |
| D. | Chromosome, genome, nucleotide, gene |
| Answer» B. Nucleotide, gene, chromosome, genome | |
| 65. |
Which of the following can be diagnosed by amniocentesis? |
| A. | Down's syndrome |
| B. | Cystic Fibrosis |
| C. | Sickle cell anemia |
| D. | All of these |
| Answer» E. | |
| 66. |
In which stage of genetic engineering a probe is used? |
| A. | Cleaving DNA |
| B. | Recombining DNA |
| C. | Cloning |
| D. | Screening |
| Answer» E. | |
| 67. |
The extra chromosomal, self-replicating, closed, double stranded and circular DNA molecule is generally termed as __________ |
| A. | Chromosome |
| B. | Plasmid |
| C. | Genomic DNA |
| D. | Bacteriophage |
| Answer» C. Genomic DNA | |
| 68. |
Sex determination in Drosophila involves |
| A. | alternate splicing |
| B. | methylation |
| C. | gene amplification |
| D. | none of these |
| Answer» B. methylation | |
| 69. |
The order for the construction of a cDNA fragment from mRNA is to |
| A. | bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase |
| B. | treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT |
| C. | digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase |
| D. | bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase |
| Answer» B. treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT | |
| 70. |
During DNA profiling, DNA nucleotides hybridized with the probe can be detected through ____________ |
| A. | Electrophoresis |
| B. | Polymerase chain reaction |
| C. | Autoradiography |
| D. | Hybridoma |
| Answer» D. Hybridoma | |
| 71. |
Which of these genes codes for a protein that plays a role in growth? |
| A. | DCP1 |
| B. | SCLC6A4 |
| C. | KRTHA1 |
| D. | GH1 |
| Answer» E. | |
| 72. |
A frameshift mutation occurs in the sigma subunit gene transcribed as part of SPOl early genes. What will be the immediate result? |
| A. | Early genes will not be translated |
| B. | Middle genes will not be transcribed |
| C. | The bacterial host will lyse immediately |
| D. | Infection will proceed as usual |
| Answer» D. Infection will proceed as usual | |
| 73. |
In the presence of high levels of tryptophan |
| A. | attenuator allows transcription of trp structural genes |
| B. | attenuator propogates transcription |
| C. | attenuator terminates transcription |
| D. | none of the above |
| Answer» D. none of the above | |
| 74. |
The TP53 gene of chromosome 17 codes for a protein __________ . |
| A. | that plays a role in the digestive process |
| B. | involved in glucose transport |
| C. | involved in the regulation of the cell cycle |
| D. | that is like a white blood cell protein |
| Answer» D. that is like a white blood cell protein | |
| 75. |
Which of the following is not denatured by heating steps during PCR? |
| A. | DNA pol |
| B. | RNA pol |
| C. | Restriction enzymes |
| D. | DNA ligase |
| Answer» B. RNA pol | |
| 76. |
Which of the following takes place when tryptophan levels are low? |
| A. | Ribosomes quickly translates sequence 1 |
| B. | Sequence 2 is blocked before sequence 3 is transcribed |
| C. | Ribosomes pauses at the Try codons in sequence 1 |
| D. | Attenuation at the attenuator structure formed by sequences of 3 and 4 |
| Answer» D. Attenuation at the attenuator structure formed by sequences of 3 and 4 | |
| 77. |
Which of the following is the most appropriate definition of an operator? |
| A. | A non-coding, regulatory DNA sequence that is bound by RNA polymerase |
| B. | A non-coding, regulatory DNA sequence that is bound by a repressor protein |
| C. | A DNA-binding protein that regulates gene expression |
| D. | A cluster of genes that are regulated by a single promoter |
| Answer» C. A DNA-binding protein that regulates gene expression | |
| 78. |
What are genes? |
| A. | The functional unit of inheritance |
| B. | A fragment of DNA |
| C. | A portion of a chromosome |
| D. | All of the above |
| Answer» E. | |
| 79. |
Which of the following genetic diseases would be amenable to genetic engineering? |
| A. | Down's syndrome |
| B. | Muscular dystrophy |
| C. | Cystic fibrosis |
| D. | Cri du Chat |
| Answer» D. Cri du Chat | |
| 80. |
The transfer of antibiotic-resistant genes from genetically engineered bacteria to disease-causing bacteria __________ . |
| A. | would be of no concern if it occurred. |
| B. | has occurred |
| C. | can never occur |
| D. | seems unlikely |
| Answer» E. | |
| 81. |
Which of the following is a DNA element that instructs RNA polymerase where to bind on DNA? |
| A. | Operator |
| B. | Promoter |
| C. | Enhancer |
| D. | Hormone response element |
| Answer» C. Enhancer | |
| 82. |
CAP is |
| A. | Catabolite gene activator protein |
| B. | Catabolite gene repressor protein |
| C. | Anabolite gene activator protein |
| D. | Anabolite gene repressor protein |
| Answer» B. Catabolite gene repressor protein | |
| 83. |
Lac repressor dissociates from its operator when the repressor binds to |
| A. | Heat shock module |
| B. | Promoter |
| C. | Inducer |
| D. | Catabolite activator site |
| Answer» D. Catabolite activator site | |
| 84. |
Dolly, the first animal produced by cloning is a __________ |
| A. | Cow |
| B. | Sheep |
| C. | Rat |
| D. | Dog |
| Answer» C. Rat | |
| 85. |
The process of introduction of foreign DNA into an animal cell is called ____________ |
| A. | Transversion |
| B. | Conversion |
| C. | Inversion |
| D. | Transfection |
| Answer» E. | |
| 86. |
Sequencing of genomic DNA is included in __________ |
| A. | Phenotypic function |
| B. | Cellular function |
| C. | Molecular function |
| D. | Structural genomics |
| Answer» E. | |
| 87. |
DNA is methylated at |
| A. | AC sequences |
| B. | TATA sequences |
| C. | CAAT sequences |
| D. | CG sequences |
| Answer» E. | |
| 88. |
A set of overlapping DNA segments that together represent a consensus region of DNA is ____________ |
| A. | Expressed sequence tag |
| B. | Sequence tagged site |
| C. | Contig |
| D. | YAC |
| Answer» D. YAC | |
| 89. |
Production of a large number of genetically similar plants through plant tissue culture is called __________ |
| A. | Recombinant DNA technology |
| B. | Gene therapy |
| C. | Micro propagation |
| D. | Hybridoma technology |
| Answer» D. Hybridoma technology | |
| 90. |
Short DNA sequence having single occurrence in genome is ____________ |
| A. | Expressed sequence tag |
| B. | Sequence tagged site |
| C. | Contig |
| D. | YAC |
| Answer» C. Contig | |
| 91. |
Alternate splicing of RNA transcripts is a mechanism to regulate |
| A. | tubulin |
| B. | tachykinins |
| C. | DNA methylation |
| D. | leucine zippers |
| Answer» C. DNA methylation | |
| 92. |
E.cor1 is a __________ |
| A. | DNA ligase enzyme |
| B. | Restriction endonuclease |
| C. | A vector used for insulin synthesis |
| D. | A plasmid used as a vector |
| Answer» C. A vector used for insulin synthesis | |
| 93. |
The network of interactions engaged in by protein at cellular level is described in __________ |
| A. | Phenotypic function |
| B. | Cellular function |
| C. | Molecular function |
| D. | Structural genomics |
| Answer» C. Molecular function | |
| 94. |
Which of the following is not a transcription factor? |
| A. | Helix-turn-helix proteins |
| B. | Zinc finger proteins |
| C. | Leucine zipper proteins |
| D. | Steroid hormones |
| Answer» E. | |
| 95. |
Who was the first person to develop DNA finger printing? |
| A. | David Suzuki |
| B. | Khorana |
| C. | Alec Jaffreys |
| D. | Gilbert |
| Answer» D. Gilbert | |
| 96. |
Why is golden rice pale yellow in color? |
| A. | It is rich in chlorophyll |
| B. | It is rich in beta-carotene. |
| C. | It is rich in chlorophyll b. |
| D. | It is rich in phycobilins. |
| Answer» C. It is rich in chlorophyll b. | |
| 97. |
Conserved gene order can be termed as __________ |
| A. | Ortholog |
| B. | Synteny |
| C. | Paralog |
| D. | Microarray |
| Answer» C. Paralog | |
| 98. |
Collection of microscopic DNA spots attached to the solid surface are __________ |
| A. | Ortholog |
| B. | Synteny |
| C. | Paralog |
| D. | Microarray |
| Answer» E. | |
| 99. |
Which of the following statements is true about a vector? |
| A. | Plasmids, phages can be used as vectors |
| B. | All vectors are plasmids |
| C. | Cyanobacteria can also be used as vectors |
| D. | Fungi can also be used as vectors |
| Answer» B. All vectors are plasmids | |
| 100. |
Repressor molecules bind to __________ |
| A. | Operator |
| B. | Promoter |
| C. | Enhancer |
| D. | Hormone response element |
| Answer» B. Promoter | |