MCQOPTIONS
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MCQOPTIONS
This section includes 14 Mcqs, each offering curated multiple-choice questions to sharpen your Protein Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Only one cycle of rational protein design is sufficient to get a protein with desired characteristics. |
| A. | True |
| B. | False |
| Answer» C. | |
| 2. |
X-ray crystallography is not required in each and every cycle of rational protein design. |
| A. | True |
| B. | False |
| Answer» C. | |
| 3. |
Rational protein design is a high throughput protein engineering. |
| A. | True |
| B. | False |
| Answer» C. | |
| 4. |
Which step is performed after the variant protein is expressed and purified? |
| A. | Protein manipulation |
| B. | Computer-aided modeling |
| C. | Site-directed mutagenesis |
| D. | Evaluation of desired property |
| Answer» E. | |
| 5. |
Cellulose is a hybrid enzyme. |
| A. | False |
| B. | True |
| Answer» C. | |
| 6. |
In the rational design of proteins, once variant proteins are made and purified the new protein is evaluated to see if the desired property is achieved. |
| A. | False |
| B. | True |
| Answer» C. | |
| 7. |
Which of the following is the final, but an essential component of rational design? |
| A. | X-ray crystallography |
| B. | Computer modeling |
| C. | Protein assay |
| D. | Site-directed mutagenesis |
| Answer» E. | |
| 8. |
Which of the following step in rational design allows predictions to be made particularly in the realm of the effect of mutations on structure-based properties? |
| A. | Site-directed mutagenesis |
| B. | X-ray crystallography |
| C. | Protein assay |
| D. | Computer modeling |
| Answer» E. | |
| 9. |
Which of the following process involves the determination of the three-dimensional structure of a protein and representing it graphically and mathematically on a computer? |
| A. | Irrational design |
| B. | Directed evolution |
| C. | Natural evolution |
| D. | Rational design |
| Answer» E. | |
| 10. |
Which of the following is a strategy in protein engineering attempts to create improved protein molecules based on the three-dimensional structure and the relationship between structure and function? |
| A. | Natural evolution |
| B. | Directed evolution |
| C. | Irrational design |
| D. | Rational design |
| Answer» E. | |
| 11. |
Which of the following mutation in subtilisin makes it resistant to inactivation by bleach? |
| A. | Serine 32 glycine |
| B. | Alanine 33 methionine |
| C. | Cysteine 23 valine |
| D. | Methionine 22 alanine |
| Answer» E. | |
| 12. |
Subtilisin (a protease) is inactivated by bleach due to the presence of which amino acid? |
| A. | Cysteine at 23 |
| B. | Serine at 32 |
| C. | Alanine 33 |
| D. | Methionine at 22 |
| Answer» E. | |
| 13. |
Which of the following is done for the directed evolution of the proteins with an unknown structure? |
| A. | Site-directed mutagenesis |
| B. | Specific mutations |
| C. | Non-random mutations |
| D. | Random changes (mutations) |
| Answer» E. | |
| 14. |
In rational design, knowledge of the structure and function of the protein is taken into consideration. |
| A. | False |
| B. | True |
| Answer» C. | |